Cell imaging techniques : methods and protocols

A diverse collection of state-of-the-art methods for the microscopic imaging of cells and molecules. The authors cover a wide spectrum of complimentary techniques, including such methods as fluorescence microscopy, electron microscopy, atomic force microscopy, and laser scanning cytometry. Additional readily reproducible protocols on confocal scanning laser microscopy, quantitative computer-assisted image analysis, laser-capture microdissection, microarray image scanning, near-field scanning optical microscopy, and reflection contrast microscopy round out this eclectic collection of cutting-edge imaging techniques now available. The authors also discuss preparative methods for particles and cells by transmission electron microscopy.

Diana P. BratuGuy Cox and Eleanor KableMichael Wm. Roe and Jerome F. Fiekers and Louis H. Philipson and Vytautas P. BindokasMaria Stern and Douglas J. Taatjes and Brooke T. MossmanRobert M. ZuckerMarilyn P. Wadsworth and Burton E. Sobel and David J. Schneider and Wendy Tra and Hans van Hirtum and Douglas J. TaatjesThomas O. Moninger and Randy A. Nessler and Kenneth C. MoorePiotr Pozarowski and Elena Holden and Zbigniew DarzynkiewiczDavid A. Rew and Gerrit Woltmann and Davinder KaurVirginia Espina and John Milia and Glendon Wu and Stacy Cowherd and Lance A. LiottaChristopher B. Manning and Brooke T. Mossman and Douglas J. TaatjesSabita K. Murthy and Douglas J. DemetrickLatha Ramdas and Wei ZhangMichael Hausmann and Birgit Perner and Alexander Rapp and Leo Wollweber and Harry Scherthan and Karl-Otto GreulichBhanu P. JenaSang-Joon Cho and Bhanu P. JenaKevin D. CostaF. A. Prins and I. Cornelese-ten Velde and E. de HeerTeresa Ruiz and Michael RadermacherMichael Radermacher and Teresa RuizDimitri Vanhecke and Peter Eggli and Werner Graber and Daniel Studer

Preface	p. v
Contributors	p. ix
Color Plates	p. xiii
1 Molecular Beacons: Fluorescent Probes for Detection of Endogenous mRNAs in Living Cells	p. 1
2 Second-Harmonic Imaging of Collagen	p. 15
3 Visualizing Calcium Signaling in Cells by Digitized Wide-Field and Confocal Fluorescent Microscopy	p. 37
4 Multifluorescence Labeling Techniques and Confocal Laser Scanning Microscopy on Lung Tissue	p. 67
5 Evaluation of Confocal Microscopy System Performance	p. 77
6 Quantitative Analysis of Atherosclerotic Lesion Composition in Mice	p. 137
7 Applications of Microscopy to Genetic Therapy of Cystic Fibrosis and Other Human Diseases	p. 153
8 Laser Scanning Cytometry: Principles and Applications	p. 165
9 Near-Clinical Applications of Laser Scanning Cytometry	p. 193
10 Laser Capture Microdissection	p. 213
11 Analysis of Asbestos-Induced Gene Expression Changes in Bronchiolar Epithelial Cells Using Laser Capture Microdissection and Quantitative Reverse Transcriptase-Polymerase Chain Reaction	p. 231
12 New Approaches to Fluorescence In Situ Hybridization	p. 237
13 Microarray Image Scannin	p. 261
14 Near-Field Scanning Optical Microscopy in Cell Biology and Cytogenetics	p. 275
15 Porosome: The Fusion Pore Revealed by Multiple Imaging Modalities	p. 295
16 Secretory Vesicle Swelling by Atomic Force Microscopy	p. 317
17 Imaging and Probing Cell Mechanical Properties With the Atomic Force Microscope	p. 331
18 Reflection Contrast Microscopy: The Bridge Between Light and Electron Microscopy	p. 363
19 Three-Dimensional Analysis of Single Particles by Electron Microscopy: Sample Preparation and Data Acquisition	p. 403
20 Three-Dimensional Reconstruction of Single Particles in Electron Microscopy: Image Processing	p. 427
21 A New Microbiopsy System Enables Rapid Preparation of Tissue for High-Pressure Freezing	p. 463
Index	p. 479

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