Available:*
Library | Item Barcode | Call Number | Material Type | Item Category 1 | Status |
|---|---|---|---|---|---|
Searching... | 30000004583849 | TP248.65.P76 B82 2006 | Open Access Book | Book | Searching... |
On Order
Summary
Summary
The ability to introduce non-canonical amino acids in vivo has greatly expanded the repertoire of accessible proteins for basic research and biotechnological application.
Here, the different methods and strategies to incorporate new or modified amino acids are explained in detail, including a lot of practical advice for first-time users of this powerful technique.
Novel applications in protein biochemistry, genomics, biotechnology and biomedicine made possible by the expansion of the genetic code are discussed and numerous examples are given.
Essential reading for all molecular life scientists who want to stay ahead in their research.
Author Notes
Nediljko Budisa is a group leader at the Max-Planck-Institute of Biochemistry in Martinsried near Munich (Germany). He studied Chemistry and Biology at the University of Zagreb (Croatia) before joining the group of Nobel Prize Winner Robert Huber at Martinsried to obtain his PhD degree. During postdoctoral work with R. Huber and L. Moroder he led an independent research team in protein engineering. In 2004, Dr. Budisa received the BioFuture Award of the German Federal Ministry for Research and Education.
Table of Contents
| Introduction |
| Classical Approaches for Protein ModificationProtein Total Synthesis |
| Basic Definitions and Taxonomy |
| History Of An Expanded Amino Acid Repertoiren |
| oncanonical Amino Acids as Tools for Studying Metabolism |
| Basic Features Of The Cellular Translation Apparatus |
| Ribosome-mediated Protein Synthesist |
| RNAs and tRNA SynthetasesTranslational Proofreading |
| Ribosomal Decoding, Codon Bias and Translational Fidelity |
| Context-dependent RecodingPost-translational Modifications |
| Genetic Code Organisation And Protein Structure |
| The Universal Genetic Code |
| Natural Variations in Codon Assignment |
| Codon Reassignment and Codon Ambiguity |
| Reprogramming The Cellular Translation Machinery |
| Enzyme Specificity and Code Interpretation |
| Reassigning Coding and Non-Coding UnitsIn vitro Chemical and Enzymatic tRNA Aminoacylation |
| Novel Codon-Anticodon Base Pairs |
| Stop Codon Takover |
| Suppression-Based Methods |
| Future Approaches |
| An Expanded Genetic Codeh |
| ybrid Translation Systems |
| Metabolic Engineering for Novel Amino Acids |
| Evolutionary Considerations |
| Applications For Reprogrammed Cellular Translation |
| Structural BiologyAtomic Mutations |
| Protein Folding and StabilityFluorine and NMRDe novo Design of Protein ScaffoldsProtein-based Sensors |
| Analogues, Isosteres and Surrogates for Biomedicine |
