Cover image for Biopolymer methods in tissue engineering
Biopolymer methods in tissue engineering
Methods in molecular biology ; 238
Publication Information:
Totowa, New Jersey: Humana Press, 2004
Physical Description:
xiii, 257 p. : ill. (some col.) ; 23 cm.


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Material Type
30000010167487 R857.T55 B564 2004 Open Access Book

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There is an urgent need to develop new approaches to treat conditions as- ciated with the aging global population. The surgeon's approach to many of these problems could be described as having evolved through three stages: Removal: Traditionally, diseased or badly damaged tissues and structures might simply be removed. This was appropriate for limbs and non-essential organs, but could not be applied to structures that were critical to sustain life. An additional problem was the creation of disability or physical deformity that in turn could lead to further complications. Replacement: In an effort to treat wider clinical problems, or to overcome the limitations of amputation, surgeons turned to the use of implanted materials and medical devices that could replace the functions of biological structures. This field developed rapidly in the 1960s and 1970s, with heart valve and total joint replacement becoming common. The term "biomaterial" was used increasingly to describe the materials used in these operations, and the study of biomaterials became one of the first truly interdisciplinary research fields. Today, biomaterials are employed in many millions of clinical procedures each year and they have become the mainstay of a very successful industry.

Table of Contents

Minna Kellomaki and Pertti TormalaRaphael Gorodetsky and Akiva Vexler and Lilia Levdansky and Gerard MarxCarlo Soranzo and Davide Renier and Alessandra PavesioSteven H. Elder and Dana L. Nettles and Joel D. BumgardnerHyun D. Kim and John M. Wozney and Rebecca H. LiManuela E. Gomes and Patricia B. Malafaya and Rui L. ReisMarcy WongYang Cao and Tristan I. Croll and Justin J. Cooper-White and Andrea J. O'Connor and Geoffrey W. StevensJennifer L. WestAntonio J. Salgado and Manuela E. Gomes and Olga P. Coutinho and Rui L. ReisGordana Vunjak-Novakovic and Milica RadisicAileen Crawford and Sally C. DickinsonDavid A. Lee and Ivan MartinSally Roberts and Janis MenagePaul V. HattonJohn Hunt and Deborah HeggartyWa'el Kafienah and Trevor J. SimsIvan Martin and Oliver FrankJohn Kisiday and Alex Kerin and Alan Grodzinsky
Prefacep. v
Contributorsp. xi
List of Color Platesp. xv
1 Processing of Resorbable Poly-[alpha]-Hydroxy Acids for Use as Tissue-Engineering Scaffoldsp. 1
2 Fibrin Microbeads (FMB) As Biodegradable Carriers for Culturing Cells and for Accelerating Wound Healingp. 11
3 Synthesis and Characterization of Hyaluronan-Based Polymers for Tissue Engineeringp. 25
4 Synthesis and Characterization of Chitosan Scaffolds for Cartilage-Tissue Engineeringp. 41
5 Characterization of a Calcium Phosphate-Based Matrix for rhBMP-2p. 49
6 Methodologies for Processing Biodegradable and Natural Origin Scaffolds for Bone and Cartilage Tissue-Engineering Applicationsp. 65
7 Alginates in Tissue Engineeringp. 77
8 Production and Surface Modification of Polylactide-Based Polymeric Scaffolds for Soft-Tissue Engineeringp. 87
9 Modification of Materials With Bioactive Peptidesp. 113
10 Isolation and Osteogenic Differentiation of Bone-Marrow Progenitor Cells for Application in Tissue Engineeringp. 123
11 Cell Seeding of Polymer Scaffoldsp. 131
12 Chondrocyte Isolation, Expansion, and Culture on Polymer Scaffoldsp. 147
13 Bioreactor Culture Techniques for Cartilage-Tissue Engineeringp. 159
14 Microscopic Methods for the Analysis of Engineered Tissuesp. 171
15 Transmission Electron Microscopy of Tissue-Polymer Constructsp. 197
16 Application of Microscopic Methods for the Detection of Cell Attachment to Polymersp. 207
17 Biochemical Methods for the Analysis of Tissue-Engineered Cartilagep. 217
18 Real-Time Quantitative RT-PCR Assaysp. 231
19 Mechanical Testing of Cell-Material Constructs: A Reviewp. 239
Indexp. 255