Cover image for Forensic and clinical applications of solid phase extraction
Forensic and clinical applications of solid phase extraction
Forensic science and medicine
Publication Information:
Totowa, NJ: Humana Press, 2004


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30000004735613 HV8073 F67 2004 Open Access Book

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This complete laboratory reference manual explains the principles behind solid phase extraction (SPE) and provides readily reproducible protocols for solving extraction problems in forensic and clinical chemistry. Numerous actual chromatograms, based on original research and diverse applications, demonstrate the technique and the results that can be achieved. Extensive appendices allow fast access to frequently needed information on reagents, the preparation of solutions and buffers, milliequivalent and millimode calculations, buffers and pKa for SPE, and a complete RapidTrace¬ģ technical manual. Each proven protocol is described in step-by-step detail and contains an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and on avoiding known pitfalls.

Table of Contents

Prefacep. v
Dedicationp. vii
Acknowledgmentsp. xi
Chapter 1 Introduction to Solid Phase Extractionp. 1
Chapter 2 Silica-Based Solid Phase Extractionp. 41
Chapter 3 The Chemistry of Bonded Silicasp. 55
Chapter 4 Bonded Phase Sorbent Synthesisp. 63
Chapter 5 Strategies for the Use of Solid Phase Extractionp. 91
Chapter 6 Optimizing Solid Phase Extraction Methodsp. 109
Chapter 7 Separation of Analytes from Their Matricesp. 129
Chapter 8 Solid Phase Extraction Troubleshootingp. 147
Chapter 9 Drug Methods for the Toxicology Labp. 169
Chapter 10 Selected Solid Phase Extraction Applications in Drug Analysisp. 245
Chapter 11 General Methods for Drug Isolationp. 281
Chapter 12 Chemical Derivatizationp. 297
Appendix A Glossaryp. 311
Appendix B Reagentsp. 315
Appendix C Preparation of Solutions and Buffersp. 317
Appendix D Milliequivalent and Millimole Calculationsp. 321
Appendix E Selected Solvent Polarity and Property Scalep. 323
Appendix F Buffers for Solid Phase Extractionp. 325
Appendix G pK[subscript a] Reference for Solid Phase Extractionp. 327
Appendix H RapidTrace Operating Guidep. 335
Appendix I Further Readingp. 343
Indexp. 351