Title:
Biotechnology : DNA to protein : a laboratory project in molecular biology
Personal Author:
Publication Information:
New York : McGraw-Hill, 2002
ISBN:
9780072416640
General Note:
Available in folio size (QH506 T54 2002 f c.2)
Available:*
Library | Item Barcode | Call Number | Material Type | Item Category 1 | Status |
|---|---|---|---|---|---|
Searching... | 30000005207992 | QH506 T54 2002 | Open Access Book | Book | Searching... |
Searching... | 30000010052358 | QH506 T54 2002 | Open Access Book | Book | Searching... |
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Summary
Summary
This one-semester, project-based laboratory manual gives junior/senior level students the opportunity to characterize the enzyme alpha-amylase. As students proceed through the sequenced experiments, they will learn the principles of DNA, RNA, and protein structure by using modern-day laboratory techniques. Genetics, cell biology and organic chemistry are prerequisites.
Table of Contents
| Examine Enzyme Activity of alpha-Amylase |
| 1 Starch Plate Assay |
| 2 Quantitative Enzyme Assay |
| 3 Factors Affecting Enzyme Function Examine alpha-Amylase Proteins |
| 4 Analysis of Protein Structure Using RasMol |
| 5 Analysis of alpha-Amylase Proteins |
| 5A SDS-PAGE |
| 5B Western Blotting Examine DNA Structure |
| 6 Analysis of DNA Structure Using RasMol |
| 7 Isolation of Chromosomal DNA from Bacillus licheniformis Find the alpha-Amylase Gene |
| 8 PCR Amplification and Labeling of Probe DNA |
| 9 Southern Hybridization |
| 9A Restriction Enzyme Cleavage of Chromosomal DNA |
| 9B Denaturation and Transfer of DNA to a Membrane |
| 9C Southern Hybridization and Detection Clone the alpha-Amylase Gene |
| 10 Cloning the alpha-Amylase Gene |
| 10A Cleavage of Chromosomal DNA |
| 10B Cleavage of Plasmid DNA |
| 10C Ligation of Chromosomal and Plasmid DNA |
| 10D Transformation |
| 10E Identification of alpha-Amylase Clones Analyze alpha-Amylase Clones |
| 11 Verification and Mapping of alpha-Amylase Clones |
| 11A Verification of alpha-Amylase Clones Using PCR |
| 11B Isolation of Plasmid DNA from alpha-Amylase Clones |
| 11C Restriction Cleavage and Mapping of alpha-Amylase Plasmid DNA |
| 11D Preservation of Recombinant Strains |
| 11E Southern Analysis of alpha-Amylase Plasmid DNA |
| 12 Enzyme Activity of alpha-Amylase Clones |
