Title:
Guide to yeast genetics and molecular biology
Series:
Methods in enzymology; 194
Publication Information:
San Diego, Calif : Academic Pr., 1991
ISBN:
9780121820954
Available:*
Library | Item Barcode | Call Number | Material Type | Item Category 1 | Status |
---|---|---|---|---|---|
Searching... | 30000001435787 | QK617.5 .G94 1991 | Open Access Book | Book | Searching... |
On Order
Summary
Summary
This guide presents a compilation of the protocols and procedures that have made saccharomyce cerevisiae such a facile system for all researchers in yeast molecular and cell biology. It is designed to meet the needs of the newcomer, experienced researcher, and graduate student. Alternative procedures in genetics, cloning, cell biology, microscopy, and organelle separation (ie nuclei) are compared and their relative merits and disadvantages clearly described.
Table of Contents
Basic Methods of Yeast GeneticsF. Sherman |
Getting Started with YeastF. Sherman and J. Hicks |
Micromanipulation and Dissection of AsciF. Sherman and P. Wakem |
Mapping Yeast GenesS.L. Gerring and C. Connelly and P. Hieter |
Positional Mapping of Genes by Chromosome Blotting and Chromosome FragmentationG.F. Sprague |
Assay of Yeast Mating ReactionY. Kassir and G. Simchen |
Monitoring Meiosis and Sporulation in Saccharomyces cerevisiaeR.E. Esposito and M. Dresser and M. Breitenbach |
Identifying Sporulation Genes |
Visualizing Synaptonemal Complexes |
and Large-Scale Spore and Spore Wall PurificationI. Herskowitz and R.E. Jensen |
Putting the HO Gene to Work: Practical Uses for Mating-Type SwitchingB. Rockmill and E.J. Lambie and G.S. Roeder |
Spore EnrichmentT.D. Fox and L.S. Folley and J.J. Mulero and T.W. McMullin and P.E. Thorsness and L.O. Hedin and M.C. Costanzo |
Analysis and Manipulation of Yeast Mitochondrial Genes |
Cloning and Recombinant DNAP. Philippsen and A. Stotz and C. Scherf |
DNA of Saccharomyces cerevisiaeD.M. Becker and L. Guarente |
High-Efficiency Transformation of Yeast by ElectroporationJ.A. Heinemann and G.F. Sprague |
Transmission of Plasmid DNA to Yeast by Conjugation with BacteriaM.D. Rose and J.R. Broach |
Cloning Genes by Complementation in YeastR.A. Young and R.W. Davis |
Gene Isolation with |