Available:*
Library | Item Barcode | Call Number | Material Type | Item Category 1 | Status |
---|---|---|---|---|---|
Searching... | 30000010150556 | QP552.M44 S774 2006 | Open Access Book | Book | Searching... |
On Order
Summary
Summary
While the genomic revolution has quickly led to the deposit of more than 30,000 structures in the protein data bank (PDB), less than one percent of those contributions represent membrane proteins despite the fact that membrane proteins constitute some 20 percent of all proteins. This discrepancy becomes significantly troublesome when it is coupled with the fact that 60 percent of current drugs are based on targeting this group of proteins, a trend that does not seem likely to reverse.
Structural Genomics on Membrane Proteins provides an excellent overview on novel research in bioinformatics and modeling on membranes, as well as the latest technological developments being employed in expression, purification, and crystallography to obtain high-resolution structures on membrane proteins. This cutting-edge work also explains the difficulties facing researchers--both technical and ethical--that have slowed the process.
Structural Genomics on Membrane Proteins provides researchers with an unprecedented look at the novel technologies that will ultimately allow them to conquer the last frontier in structural biology, leading to accelerated breakthroughs in drug discovery.
Author Notes
Kenneth H. Lundstrom
Table of Contents
Chapter 1 Introduction | p. 1 |
Chapter 2 Bioinformatics in Membrane Protein Analysis | p. 5 |
Chapter 3 Prokaryotic Membrane Transport Proteins: Amplified Expression and Purification | p. 21 |
Chapter 4 Membrane Protein Production Strategies for Structural Genomics | p. 43 |
Chapter 5 Refolding of Membrane Proteins for Large-Scale Production | p. 57 |
Chapter 6 Crystallization of Membrane Proteins | p. 73 |
Chapter 7 Signaling through Membrane Proteins | p. 89 |
Chapter 8 Expression of Membrane Proteins in Yeasts | p. 115 |
Chapter 9 Expression of Functional Membrane Proteins in the Baculovirus-Insect Cell System: Challenges and Developments | p. 153 |
Chapter 10 Expression of Membrane Proteins in Mammalian Cells | p. 169 |
Chapter 11 Solubilization and Purification of Membrane Proteins | p. 179 |
Chapter 12 Fluorescent Labelling of Membrane Proteins in Living Cells | p. 199 |
Chapter 13 Membrane Protein NMR | p. 211 |
Chapter 14 Miniaturization of Structural Biology Technologies - From Expression to Biophysical Analyses | p. 261 |
Chapter 15 Electron Microscopy and Atomic Force Microscopy of Reconstituted Membrane Proteins | p. 299 |
Chapter 16 Structural Genomics Networks for Membrane Proteins | p. 321 |
Chapter 17 Molecular Modelling of Membrane Proteins | p. 331 |
Chapter 18 Toward Structural Bases for GPCR Ligand Binding: A Path for Drug Discovery | p. 349 |
Index | p. 379 |