Electron microscopy : methods and protocols

Title:

Series:

Methods in molecular biology ; 369

Edition:

2nd ed.

Publication Information:

Totowa, NJ : Humana Press, 2007

Physical Description:

xv, 608 p. : ill. ; 24 cm.

ISBN:

9781588295736

Subject Term:

Electron microscopy -- Laboratory manuals

Histology -- Technique

Added Author:

Kuo, John

Available:*

Library	Item Barcode	Call Number	Material Type	Item Category 1	Status
Searching... PSZ JB	30000010179520	QH212.E4 E434 2007	Open Access Book	Book	Searching... Unknown

Hands-on experts describe in detail the key electron microscopy techniques used for examining cells, tissue, biological macromolecules, molecular structure, and their interactions. Electron Microscopy Methods and Protocols, Second Edition offers both newcomers and established researchers across experimental biology and medicine wanting to expand their repertoire a gold-standard laboratory manual of cutting-edge electron microscopy techniques - each optimized for reproducibility and robust results - today's gold-standard laboratory manual. New to this edition are sections covering Transmission Electron Microscopy (TEM), and Scanning Electron Microscopy (SEM).

John J. BozzolaJosé A. Mascorro and John J. BozzolaJohn KuoPaul WebsterHerbert K. HaglerE. Ann EllisJ. Robin HarrisKent L. McDonald and Mary Morphew and Paul Verkade and Thomas Muller-ReichertDimitri Vanhecke and Luca Studer and Daniel StuderMargaret Coughlin and William M. Brieher and Ryoma OhiDuÜan Cmarko and Karel KobernaHeinz Schwarz and Bruno M. HumbelPaul Webster and Alexandre WebsterPaul A. Sims and Jeff D. HardinTerry M. MayhewHui-Ting Chou and James E. Evans and Henning StahlbergWen Jiang and Wah ChiuPeter EngelhardtPeter Engelhardt and Jari Meriläinen and Fang Zhao and Susumu Uchiyama and Kiichi Fukui and Veli-Pekka LehtoMichael Marko and Chyong-Ere HsiehNadine Kapp and Daniel Studer and Peter Gehr and Marianne GeiserJohn J. BozzolaBrendan J. GriffinYasuhiro Utsumi and Yuzou SanoGodfried M. Roomans and Anca DragomirBeat FreyNicholas P. LockyerNicole Grignon

Preface	p. v
Acknowledgments	p. vii
1 Conventional Specimen Preparation Techniques for Transmission Electron Microscopy of Cultured Cells	p. 1
2 Processing Biological Tissues for Ultrastructural Study	p. 19
3 Processing Plant Tissues for Ultrastructural Study	p. 35
4 Microwave-Assisted Processing and Embedding for Transmission Electron Microscopy	p. 47
5 Ultramicrotomy for Biological Electron Microscopy	p. 67
6 Poststaining Grids for Transmission Electron Microscopy: Conventional and Alternative Protocols	p. 97
7 Negative Staining of Thinly Spread Biological Samples	p. 107
8 Recent Advances in High-Pressure Freezing: Equipment- and Specimen-Loading Methods	p. 143
9 Cryoultramicrotomy: Cryoelectron Microscopy of Vitreous Sections	p. 175
10 Cell-Free Extract Systems and the Cytoskeleton: Preparation of Biochemical Experiments for Transmission Electron Microscopy	p. 199
11 Electron Microscopy In Situ Hybridization: Tracking of DNA and RNA Sequences at High Resolution	p. 213
12 Correlative Light and Electron Microscopy Using Immunolabeled Resin Sections	p. 229
13 Cryosectioning Fixed and Cryoprotected Biological Material for Immunocytochemistry	p. 257
14 Fluorescence-Integrated Transmission Electron Microscopy Images: Integrating Fluorescence Microscopy With Transmission Electron Microscopy	p. 291
15 Quantitative Immunoelectron Microscopy: Alternative Ways of Assessing Subcellular Patterns of Gold Labeling	p. 309
16 Electron Crystallography of Membrane Proteins	p. 331
17 Cryoelectron Microscopy of Icosahedral Virus Particles	p. 345
18 Three-Dimensional Reconstruction of Chromosomes Using Electron Tomography	p. 365
19 Whole-Mount Immunoelectron Tomography of Chromosomes and Cells	p. 387
20 Three-Dimensional Cryotransmission Electron Microscopy of Cells and Organelles	p. 407
21 Electron Energy-Loss Spectroscopy as a Tool for Elemental Analysis in Biological Specimens	p. 431
22 Conventional Specimen Preparation Techniques for Scanning Electron Microscopy of Biological Specimens	p. 449
23 Variable Pressure and Environmental Scanning Electron Microscopy: Imaging of Biological Samples	p. 467
24 Cryoplaning Technique for Visualizing the Distribution of Water in Woody Tissues by Cryoscanning Electron Microscopy	p. 497
25 X-Ray Microanalysis in the Scanning Electron Microscope	p. 507
26 Botanical X-Ray Microanalysis in Cryoscanning Electron Microscopy	p. 529
27 Static Secondary Ion Mass Spectrometry for Biological and Biomedical Research	p. 543
28 Using SIMS and MIMS in Biological Materials: Application to Higher Plants	p. 569
Index	p. 593

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