Reporter genes : a practical guide

Reporter genes have played, and continue to play, a vital role in many areas of biological research by providing a ready means for qualitative and quantitative assessment of the activity of genes and location of gene products in different environments. This book describes practical protocols for experimentation with the most useful reporter genes for mammalian systems that are available and will concentrate on those marker genes that are currently most commonly used.

Siobhan Loughna and Deborah HendersonPhilip A. Seymour and Maike SanderMaria Limberis and Peter Bell and James M. WilsonWolfgang J. Weninger and Timothy J. MohunGeorge H. PattersonKarin Edoff and James S. Dods and Andrea H. BrandCharles G. Bailey and John E. J. RaskoSteven K. Dower and Eva E. Qwarnstrom and Endre Kiss-TothRobert M. HoffmanPritha Ray and Sanjiv Sam GambhirHarald Jockusch and Daniel Eberhard

Preface	p. v
Contributors	p. ix
1 Methodologies for Staining and Visualisation of [beta]-Galactosidase in Mouse Embryos and Tissues	p. 1
2 Immunohistochemical Detection of [beta]-Galactosidase or Green Fluorescent Protein on Tissue Sections	p. 13
3 Detection of Reporter Gene Expression in Murine Airways	p. 25
4 Three-Dimensional Analysis of Molecular Signals with Episcopic Imaging Techniques	p. 35
5 Fluorescent Proteins for Cell Biology	p. 47
6 Detection of GFP During Nervous System Development in Drosophila melanogaster	p. 81
7 Autofluorescent Proteins for Flow Cytometry	p. 99
8 Fluorescent Protein Reporter Systems for Single-Cell Measurements	p. 111
9 Subcellular Imaging of Cancer Cells in Live Mice	p. 121
10 Noninvasive Imaging of Molecular Events with Bioluminescent Reporter Genes in Living Subjects	p. 131
11 Green Fluorescent Protein as a Tracer in Chimeric Tissues: The Power of Vapor Fixation	p. 145
Index	p. 155

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Summary

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Table of Contents