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Cover image for Microbial processes and products
Title:
Microbial processes and products
Series:
Methods in cell biology ; 18
Publication Information:
Totowa, NJ : Humana Press, 2005
ISBN:
9781588295484
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Item Category 1
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30000010082783 TP248.27.M53 M524 2005 Open Access Book Book
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Summary

Summary

The development of biotechnology over the last 20 years, and particularly the use of recombinant DNA techniques, has rapidly expanded the opportu- ties for human benefits from living resources. Efforts to reduce pollution, p- vent environmental damage, combat microbial infection, improve food production, and so on can each involve fermentation or the environmental - lease of microorganisms. Many products of fermentation technology, such as alcoholic beverages, bread, antibiotics, amino acids, vitamins, enzymes, and others, have been influenced by the progress of recombinant DNA techniques. The development of new products or the more efficient manufacturing of those already being produced often involve the use of microorganisms as cell fac- ries for many productions and biotransformations. Microbial Processes and Products is intended to provide practical expe- mental laboratory procedures for a wide range of processes and products me- ated by microorganisms. Although not an exhaustive treatise, it provides a detailed "step-by-step" description of the most recent developments in such applied biotechnological processes. The detailed protocols we provide are cross-referenced in the Notes section, contain critical details, lists of problems and their troubleshooting, as well as safety recommendations that may not n- mally appear in journal articles and can be particularly useful for those un- miliar with specific techniques.


Table of Contents

Sarad R. ParekhMichelle SergentMarta Rodríguez-Sáiz and Juan-Luis de la Fuente and José-Luis BarredoWolfgang MinasJosé M. Luengo and Belén García and Ángel Sandoval and Elsa Arias-Barrau and Sagrario Arias and Francisco Bermejo and Elías R. OliveraCarmen Méndez and José A. SalasPaloma Liras and Juan F. MartínMaria Margarida Diogo and João António Queiroz and Duarte Miguel F. PrazeresMutant Masato Ikeda and Junko Ohnishi and Satoshi MitsuhashiChristoph Wittmann and Elmar HeinzleHeiko Barg and Marco Malten and Martina Jahn and Dieter JahnHeidi Sisniega and José-Luis del Río and María-José Amaya and Ignacio FausEnrique A. Iturriaga and Tamás Papp and Jesper Breum and José Arnau and Arturo P. EslavaHans Visser and Gerhard Sandmann and Jan C. VerdoesPaul D. Fraser and Peter M. BramleyMaría A. Santos and Laura Mateos and Karl-Peter Stahmann and José-Luis RevueltaThomas P. WestStefania Cresti and Cesira L. Galeotti and Serena Schippa and Gian Maria Rossolini and Maria C. ThallerBart Weimer and Yi Xie and Lan-szu Chou and Adele CutlerTammi L. Olineka and Apostolos Spiropoulos and Paula A. Mara and Linda F. BissonFrederic Leroy and Luc De VuystEduardo J. Schiffrin and Nabila Ibnou-Zekri and Jean M. Ovigne and Thierry von der Weid and Stephanie BlumBart Weimer and Ben DiasAxel Schippers and Klaus BoseckerMarion Paterson-Beedle and Lynne E. MacaskieVera Weihs and Christine RohdeVera WeihsUwe Fitzner
Prefacep. v
Contributorsp. xi
1 Development of Improved Strains and Optimization of Fermentation Processesp. Lei Han
2 Experimental Design in Microbiologyp. Guillaume E.
3 Metabolic Engineering of Acremonium chrysogenum to Produce Deacetoxycephalosporin C and Bioconversion to 7-Aminodeacetoxycephalosporanic Acidp. 41
4 Production of Erythromycin With Saccharopolyspora erythraeap. 65
5 The Phenylacetyl-CoA Catabolon: Genetic, Biochemical, and Biotechnological Approachesp. 91
6 Recombinant Microorganisms for the Biosynthesis of Glycosylated Antitumor Compoundsp. 131
7 Assay Methods for Detection and Quantification of Antimicrobial Metabolites Produced by Streptomyces clavuligerusp. 149
8 Purification of Plasmid DNA Vectors Produced in Escherichia coli for Gene Therapy and DNA Vaccination Applicationsp. 165
9 Genome Breeding of an Amino Acid-Producing Corynebacterium glutamicump. 179
10 Metabolic Activity Profiling by 13C Tracer Experiments and Mass Spectrometry in Corynebacterium glutamicump. 191
11 Protein and Vitamin Production in Bacillus megateriump. 205
12 Strategies for Large-Scale Production of Recombinant Proteins in Filamentous Fungip. 225
13 Strain and Culture Conditions Improvement for ß-Carotene Production With Mucorp. 239
14 Xanthophylls in Fungi: Metabolic Engineering of the Astaxanthin Biosynthetic Pathway in Xanthophyllomyces dendrorhousp. 257
15 Methodologies for the Analysis of Fungal Carotenoidsp. 273
16 Insertional Mutagenesis in the Vitamin B2 Producer Fungus Ashbya gossypiip. 283
17 Improved Polysaccharide Production Using Strain Improvementp. 301
18 Use of the Morganella morganii phoC Gene as Reporter in Bacterial and Yeast Hostsp. 313
19 Gene Expression Arrays in Foodp. 333
20 Optimization of Proteome Analysis for Wine Yeast Strainsp. 345
21 Bacteriocin-Producing Strains in a Meat Environmentp. 369
22 In Vitro and In Vivo Interactions of Nonpathogenic Bacteria With Immunocompetent Cellsp. 381
23 Volatile Sulfur Detection in Fermented Foodsp. 397
24 Bioleaching: Analysis of Microbial Communities Dissolving Metal Sulfidesp. 405
25 Use of PhoN Phosphatase to Remediate Heavy Metalsp. 413
26 Safe Dispatch and Transport of Biological Materialp. 437
27 How to Deposit Biological Material for Patent Purposesp. 451
28 Laws and Regulations for the Protection of Biotechnological Inventionsp. 465
Indexp. 495
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