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Library | Item Barcode | Call Number | Material Type | Item Category 1 | Status |
|---|---|---|---|---|---|
Searching... | 30000010106733 | SB106.B56 M64 2004 | Open Access Book | Book | Searching... |
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Summary
Summary
Here, authors from academia and industry provide an exciting overview of current production technologies and the fascinating possibilities for future applications. Topics include chloroplast-derived antibodies, biopharmaceuticals and edible vaccines, production of antibodies in plants and plant cell suspension cultures, production of spider silk proteins in plants, and glycosylation of plant produced proteins. The whole is rounded off by chapters on the demands and expectations made on molecular farming by pharmaceutical corporations and the choice of crop species in improving recombinant protein levels. Of interest to biotechnologists, gene technologists, molecular biologists and protein biochemists in university as well as the biotechnological and pharmaceutical industries.
Author Notes
Prof. Dr. Rainer Fischer, 45, currently Department Head of the Institute for Molecular Biotechnology (RWTH Aachen, Germany, 80 employees) and Senior Executive Director of the Fraunhofer Institute for Molecular Biology and Applied Ecology (170 employees). His research covers many areas of Molecular Biotechnology including Genomics, Proteomics, Cellomics, Protein Engineering, Molecular Medicine, Immunology, Virology, Plant Biotechnology, as well as the Production and Purification of Recombinant Proteins.
Within the last 15 years Prof. Dr. Rainer Fischer published over 90 research articles in leading international journals, 35 book chapters and established a strong global academic and industrial network. He is also well known within the scientific arenas for his international scientific talks, is on the editorial board of two highly ranked scientific journals and is reviewer for more than 20 journals. He has filed over 25 different patent applications and successfully secured more than 100 million Euros in financial support. Prof. Fischer serves as reviewer for multiple national and international grant agencies (EC, NATO and NSF), he is an adviser for four international Biotech companies and an active board member for two start-up companies.
Table of Contents
| Preface |
| 1 Efficient and Reliable Production of Pharmaceuticals in AlfalfaMarc-Andre D'Aoust and Patrice Lerouge and Ursula Busse and Pierre Bilodeau and Sonia Trepanier and Veronique Gomord and Loic Faye and Louis-Philippe Vezina |
| 1.1 Introduction |
| 1.2 Alfalfa-specific Expression Cassettes |
| 1.3 Alfalfa Transformation Methods |
| 1.4 Characteristics of Alfalfa-derived Pharmaceuticals |
| 1.5 Industrial Production of Recombinant Proteins in Alfalfa |
| 1.5.1 Ramping Up Alfalfa Biomas |
| 1.5.2 Alfalfa Harvest, and Recovery of Recombinant Molecules |
| 1.6 Conclusions |
| References |
| 2 Foreign Protein Expression Using Plant Cell Suspension and Hairy Root CulturesFiona S. Shadwick and Pauline M. Doran |
| 2.1 Foreign Protein Production Systems |
| 2.2 Production of Foreign Proteins Using Plant Tissue Culture |
| 2.2.1 Suspended Cell Cultures |
| 2.2.2 Hairy Root Cultures |
| 2.2.3 Shooty Teratoma Cultures |
| 2.2.4 Scale-up Considerations for Different Forms of Plant Tissue Culture |
| 2.3 Strategies for Improving Foreign Protein Accumulation and Product Recovery in Plant Tissue Culture |
| 2.3.1 Expression Systems |
| 2.3.1.1 Modifications to Existing Expression Constructs |
| 2.3.1.2 Transient Expression Using Viral Vectors |
| 2.3.2 Secretion of Foreign Proteins |
| 2.3.3 Foreign Protein Stability |
| 2.3.3.1 Stability Inside the Cells |
| 2.3.3.2 Stability Outside the Cells |
| 2.3.3.3 Medium Additives |
| 2.3.3.4 Medium Properties |
| 2.3.4 Bioprocess Developments |
| 2.3.4.1 Product Recovery from the Medium |
| 2.3.4.2 Oxygen Transfer and Dissolved Oxygen Concentration |
| 2.4 Conclusions |
| References |
| 3 Novel Sprouting Technology for Recombinant Protein ProductionKimmo Koivu |
| 3.1 Introduction |
| 3.2 Biology of Sprouting |
| 3.2.1 Structure and Content of Dicotyledonous and Monocotyledonous Seeds |
| 3.2.2 Germination |
| 3.2.3 The Sprout |
| 3.2.4 Rubisco Synthesis |
| 3.2.5 Rubisco Promoters |
| 3.2.6 Inhibition of Endogenous Gene Expression |
| 3.3 Expression Cassette Design |
| 3.4 Sprouting Equipment |
| 3.5 Sprouting Conditions |
| 3.5.1 Sterilization |
| 3.5.2 Sprouting Time and Temperature |
| 3.5.3 Light |
| 3.5.4 Inhibition of Endogenous Gene Expression |
| 3.5.5 Growth Regulators |
| 3.5.6 Nitrogen Fertilizer |
| 3.5.7 Seed Production |
| 3.6 Yield Estimates and Benefits of Sprouting Technology in Protein Production |
| 3.6.1 Yield Estimates |
| 3.6.2 Quality and Environmental Aspects |
| References |
| 4 Monocot Expression Systems for Molecular FarmingPaul Christou and Eva Stoger and Richard M. Twyman |
| 4.1 Introduction |
| 4.2 Cereal Production Crops |
| 4.3 Technical Aspects of Molecular Farming in Cereals |
| 4.3.1 Cereal Transformation |
| 4.3.2 Expression Construct Design |
| 4.3.3 Production Considerations for Cereals |
| 4.4 Examples of Recombinant Proteins Produced in Cereals |
| 4.4.1 ProdiGene and Maize |
| 4.4.2 Recombinant Proteins Expressed in Rice |
| 4.4.3 Recombinant Proteins Produced in Wheat |
| 4.4.4 Recombinant Proteins Produced in Barley |
| 4.5 Conclusions |
| References |
| 5 The Field Evaluation of Transgenic Crops Engineered to Produce Recombinant ProteinsJim Brandle |
| 5.1 Introduction |
| 5.2 Regulation of Field-testing |
| 5.3 Design of Field Trials |
| 5.4 Results of Field Trials |
| References |
| 6 Plant Viral Expression Vectors: History and New DevelopmentsVidadi Yusibov and Shailaja Rabindran |
| 6.1 Introduction |
| 6.2 Plant RNA Viruses as Expression Vectors |
| 6.2.1 Tobacco mosaic virus (TMV) |
| 6.2.2 Potato virus X (PVX) |
| 6.2.3 Cowpea mosaic virus (CPMV) |
| 6.2.4 Alfalfa mosaic virus (AlMV) |
| 6.3 Biological Activity of Target Molecules |
| 6.4 Efficacy of Plant Virus-produced Antigens |
| 6.4.1 Vaccine Antigens |
| 6.4.2 Particle-based Vaccine Antigen Delivery |
| 6.4.3 Other Uses of Plant Virus Particles |
| 6.5 Plant Viruses as Gene Function Discovery |
