From genes to genomes : concepts and applications of DNA technology

Title:

Personal Author:

Dale, Jeremy W.

Publication Information:

Chichester, West Sussex : John Wiley & Sons, 2002

ISBN:

9780471497837

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Library	Item Barcode	Call Number	Material Type	Item Category 1	Status
Searching... PSZ JB	30000010061275	QH442 D34 2002	Open Access Book	Book	Searching... Unknown

Rapid advances in our understanding of genetics have required that new books contain topics such as the concept and theory of gene cloning, transgenics, genomics, and various other coverage of traditional and contemporary subjects.

Although there is an abundance of textbooks that cover introductory genetics and advanced courses in genetics, there is a noticeable gap at the intermediate (second year) level. In the past gene structure, function and expression were taught at final year /postgraduate level, but the rapid advances in our understanding of genetics has encouraged courses to change considerably. Over recent years these topics have filtered down the curriculum and are currently taught as core topics at second year, with a corresponding change in textbook requirements. Where once second year students were restricted to learning about the concept and theory of gene cloning, now they routinely clone genes for themselves as part of their practical assignments.

Genes to Genomics will fill the gap, cover much of the same ground as previous titles, but go further on contemporary topics like transgenics, sequence comparison and analysis of variation.
* A concise, up to date textbook that provides a balanced coverage of traditional and contemporary topics taught within intermediate courses in molecular genetics
* Jeremy Dale has a proven track record as the successful author of Molecular Genetics of Bacteria
* Genes to Genomics will include a series of feature box-outs that will examine some of the topical issues related to the scientific concepts and examples explored within the text
* A range of questions and exercises including worked examples and web-based practicals
* An accompanying web site will allow the authors to keep their audience up to date in the areas that are prone to date most rapidly between successive editions of the textbook. It will also include the illustrations and images from the textbook, in addition to worked examples, answers to questions within the book, and links to related websites of key interest.

Reviews 1

Choice Review

Dale and von Schantz (both, Univ. of Surrey, UK) offer a well-written and fairly comprehensive guide to molecular genetics techniques aimed at students who have completed an introductory course in genetics or molecular biology. The coverage ranges from established techniques like DNA cloning to recent advances including microarray analysis. The book is not a laboratory manual, but rather a description of current molecular genetics techniques. It would be a useful supplement for students taking laboratory courses in molecular genetics who need background information on the basic procedures. The content is reasonably up-to-date, although it lacks coverage of RNA interference, a technique that has become widespread over the past two years. This book would be a useful addition to libraries that have copies of Principles of Gene Manipulation, by Sandy Primrose, Richard Twyman, and Bob Old (6th ed., 2001). ^BSumming Up: Optional. Upper-division undergraduates. P. Guilfoile Bemidji State University

Preface	p. ix
1 Introduction	p. 1
2 Basic molecular biology	p. 7
2.1 Nucleic acid structure	p. 7
2.2 What is a gene?	p. 15
2.3 Information flow: gene expression	p. 16
2.4 Gene structure and organization	p. 21
3 How to clone a gene	p. 25
3.1 What is cloning?	p. 25
3.2 Overview of the procedures	p. 26
3.3 Gene libraries	p. 29
3.4 Hybridization	p. 30
3.5 Polymerase chain reaction	p. 32
3.6 Extraction and purification of nucleic acids	p. 33
3.7 Detection and quantitation of nucleic acids	p. 36
3.8 Gel electrophoresis	p. 37
4 Cutting and joining DNA	p. 41
4.1 Restriction endonucleases	p. 41
4.2 Ligation	p. 47
4.3 Modification of restriction fragment ends	p. 53
4.4 Other ways of joining DNA molecules	p. 57
5 Vectors	p. 61
5.1 Plasmid vectors	p. 61
5.2 Vectors based on the lambda bacteriophage	p. 69
5.3 Cosmids	p. 78
5.4 M13 vectors	p. 79
5.5 Expression vectors	p. 81
5.6 Vectors for cloning and expression in eukaryotic cells	p. 84
5.7 Supervectors: YACs and BACs	p. 92
5.8 Summary	p. 94
6 Genomic and cDNA libraries	p. 95
6.1 Genomic libraries	p. 96
6.2 Growing and storing libraries	p. 105
6.3 cDNA libraries	p. 106
6.4 Random, arrayed and ordered libraries	p. 113
7 Finding the right clone	p. 117
7.1 Screening libraries with gene probes	p. 117
7.2 Screening expression libraries with antibodies	p. 128
7.3 Subcloning	p. 130
7.4 Characterization of plasmid clones	p. 131
8 Polymerase chain reaction	p. 135
8.1 The PCR reaction	p. 136
8.2 PCR in practice	p. 140
8.3 Cloning PCR products	p. 144
8.4 Long-range PCR	p. 146
8.5 Reverse-transcription PCR	p. 146
8.6 Rapid amplification of cDNA ends	p. 147
8.7 Quantitative PCR	p. 149
8.8 Applications of PCR	p. 154
9 Characterization of a cloned gene	p. 159
9.1 DNA sequencing	p. 159
9.2 Databank entries and annotation	p. 167
9.3 Sequence analysis	p. 173
9.4 Sequence comparisons	p. 178
9.5 Protein structure	p. 190
9.6 Confirming gene function	p. 196
10 Analysis of gene expression	p. 201
10.1 Analysing transcription	p. 201
10.2 Methods for studying the promoter	p. 209
10.3 Regulatory elements and DNA-binding proteins	p. 213
10.4 Translational analysis	p. 217
11 Products from native and manipulated cloned genes	p. 221
11.1 Factors affecting expression of cloned genes	p. 222
11.2 Expression of cloned genes in bacteria	p. 227
11.3 Expression in eukaryotic host cells	p. 235
11.4 Adding tags and signals	p. 239
11.5 In vitro mutagenesis	p. 242
11.6 Vaccines	p. 247
12 Genomic analysis	p. 251
12.1 Genome sequencing	p. 251
12.2 Analysis and annotation	p. 261
12.3 Comparing genomes	p. 269
12.4 Genome browsers	p. 271
12.5 Relating genes and functions: genetic and physical maps	p. 273
12.6 Transposon mutagenesis and other screening techniques	p. 276
12.7 Conclusion	p. 285
13 Analysis of genetic variation	p. 287
13.1 Single nucleotide polymorphisms	p. 288
13.2 Larger-scale variations	p. 291
13.3 Other methods for studying variation	p. 293
13.4 Human genetic diseases	p. 300
13.5 Molecular phylogeny	p. 305
14 Post-genomic analysis	p. 313
14.1 Analysing transcription; transcriptomes	p. 313
14.2 Array-based methods	p. 319
14.3 Translational analysis; proteomics	p. 326
14.4 Post-translational analysis: protein interactions	p. 329
14.5 Integrative studies; systems biology	p. 332
15 Modifying organisms; transgenics	p. 333
15.1 Modification of bacteria and viruses: live vaccines	p. 333
15.2 Transgenesis and cloning	p. 337
15.3 Animal transgenesis	p. 338
15.4 Applications of transgenic animals	p. 347
15.5 Transgenic plants and their applications	p. 351
Glossary	p. 355
Bibliography	p. 373
Index	p. 375

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Table of Contents