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Cover image for Gene biotechnology
Title:
Gene biotechnology
Edition:
2nd ed.
Publication Information:
Boca Raton, FL : CRC Pr., 2004
ISBN:
9780849312885
General Note:
First ed. published under title : Methods in gene biotechnology (1997)

Available:*

Library
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Material Type
Item Category 1
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30000010041299 QH442 M57 2003 Open Access Book Book
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30000004858837 QH442 M47 2004 Open Access Book Book
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Summary

Summary

Many scientists find themselves working in the laboratory without sufficient background in current biotechnology methods. Others want to keep up with the revolution in biotechnology and the flood of new methodologies. This book provides a solution for both: a multidisciplinary approach to the methods essential to biotechnical development.

Covering state-of-the-art technologies and a broad range of practical applications, the Second Edition of Gene Biotechnology presents information that researchers and students need to understand and apply today's biotechnology techniques. It discusses a wide variety of approaches, from very basic methods to the latest, most sophisticated technologies, including gene manipulation methods. The book contains clearly detailed, step-by-step protocols with helpful troubleshooting tips. It addresses the needs of researchers in academic and commercial environments, and serves as a guide for graduate students designing, implementing, and evaluating experimental projects.

Written by a team of internationally recognized scientists, this edition offers several notable features typically lacking in other works on the subject. The book presents protocols, as well as clear and simple explanations of the key principles and concepts behind the methods. It includes useful troubleshooting guides to help solve experimental problems, presenting a single, logically organized source for all the most important new methodologies. This unique resource provides the tools for success in molecular and cellular biology research today.


Table of Contents

Chapter 1 Strategies for Novel Research Projectsp. 1
Chapter 2 Rapid Isolation of Specific cDNAs or Genes by PCRp. 15
Chapter 3 Construction and Screening of Subtracted and Complete Expression cDNA Librariesp. 31
Chapter 4 Subcloning of Genes or DNA Fragmentsp. 73
Chapter 5 Nonisotopic and Isotopic DNA or RNA Sequencingp. 99
Chapter 6 Information Superhighway and Computer Databases of Nucleic Acids and Proteinsp. 139
Chapter 7 Characterization of DNA or Genes by Southern Blot Hybridizationp. 165
Chapter 8 Gene Overexpression by Sense RNA in Mammalian Systemsp. 193
Chapter 9 Gene Underexpression in Cultured Cells and Animals by Antisense DNA and RNA Strategiesp. 223
Chapter 10 Analysis of Gene Expression at the Functional Genomic Levelp. 247
Chapter 11 Analysis of Gene Expression at the Proteomic Levelp. 265
Chapter 12 Analysis of Cellular DNA or Abundance of mRNA by Radioactivity in Situ Hybridization (RISH)p. 287
Chapter 13 Localization of DNA or Abundance of mRNA by Fluorescence in Situ Hybridization (FISH)p. 311
Chapter 14 In Situ PCR Hybridization of Low Copy Genes and in Situ RT-PCR Detection of Low Abundance mRNAsp. 323
Chapter 15 Isolation and Characterization of Genes from Genomic DNA Librariesp. 341
Chapter 16 Culture of Mouse Embryonic Stem Cells as a Model Mammalian Cell Line for Gene Expressionp. 365
Chapter 17 New Strategies for Gene Knockoutp. 375
Chapter 18 Large-Scale Expression and Purification of Recombinant Proteins in Cultured Cellsp. 409
Chapter 19 Quantitative Analysis of Functional Genome by Current Real-Time RT-PCRp. 445
Chapter 20 High-Throughput Analysis of Gene Expression by Cutting-Edge Technology--DNA Microarrays (Gene Chips)p. 461
Chapter 21 Construction and Screening of Human Antibody Libraries: Using State-of-the-Art Technology--Phage Displayp. 471
Chapter 22 Down-Regulation of Gene Expression in Mammalian Systems via Current siRNA Technologyp. 493
Indexp. 509
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