Available:*
Library | Item Barcode | Call Number | Material Type | Item Category 1 | Status |
---|---|---|---|---|---|
Searching... | 30000010041299 | QH442 M57 2003 | Open Access Book | Book | Searching... |
Searching... | 30000004858837 | QH442 M47 2004 | Open Access Book | Book | Searching... |
On Order
Summary
Summary
Many scientists find themselves working in the laboratory without sufficient background in current biotechnology methods. Others want to keep up with the revolution in biotechnology and the flood of new methodologies. This book provides a solution for both: a multidisciplinary approach to the methods essential to biotechnical development.
Covering state-of-the-art technologies and a broad range of practical applications, the Second Edition of Gene Biotechnology presents information that researchers and students need to understand and apply today's biotechnology techniques. It discusses a wide variety of approaches, from very basic methods to the latest, most sophisticated technologies, including gene manipulation methods. The book contains clearly detailed, step-by-step protocols with helpful troubleshooting tips. It addresses the needs of researchers in academic and commercial environments, and serves as a guide for graduate students designing, implementing, and evaluating experimental projects.
Written by a team of internationally recognized scientists, this edition offers several notable features typically lacking in other works on the subject. The book presents protocols, as well as clear and simple explanations of the key principles and concepts behind the methods. It includes useful troubleshooting guides to help solve experimental problems, presenting a single, logically organized source for all the most important new methodologies. This unique resource provides the tools for success in molecular and cellular biology research today.
Table of Contents
Chapter 1 Strategies for Novel Research Projects | p. 1 |
Chapter 2 Rapid Isolation of Specific cDNAs or Genes by PCR | p. 15 |
Chapter 3 Construction and Screening of Subtracted and Complete Expression cDNA Libraries | p. 31 |
Chapter 4 Subcloning of Genes or DNA Fragments | p. 73 |
Chapter 5 Nonisotopic and Isotopic DNA or RNA Sequencing | p. 99 |
Chapter 6 Information Superhighway and Computer Databases of Nucleic Acids and Proteins | p. 139 |
Chapter 7 Characterization of DNA or Genes by Southern Blot Hybridization | p. 165 |
Chapter 8 Gene Overexpression by Sense RNA in Mammalian Systems | p. 193 |
Chapter 9 Gene Underexpression in Cultured Cells and Animals by Antisense DNA and RNA Strategies | p. 223 |
Chapter 10 Analysis of Gene Expression at the Functional Genomic Level | p. 247 |
Chapter 11 Analysis of Gene Expression at the Proteomic Level | p. 265 |
Chapter 12 Analysis of Cellular DNA or Abundance of mRNA by Radioactivity in Situ Hybridization (RISH) | p. 287 |
Chapter 13 Localization of DNA or Abundance of mRNA by Fluorescence in Situ Hybridization (FISH) | p. 311 |
Chapter 14 In Situ PCR Hybridization of Low Copy Genes and in Situ RT-PCR Detection of Low Abundance mRNAs | p. 323 |
Chapter 15 Isolation and Characterization of Genes from Genomic DNA Libraries | p. 341 |
Chapter 16 Culture of Mouse Embryonic Stem Cells as a Model Mammalian Cell Line for Gene Expression | p. 365 |
Chapter 17 New Strategies for Gene Knockout | p. 375 |
Chapter 18 Large-Scale Expression and Purification of Recombinant Proteins in Cultured Cells | p. 409 |
Chapter 19 Quantitative Analysis of Functional Genome by Current Real-Time RT-PCR | p. 445 |
Chapter 20 High-Throughput Analysis of Gene Expression by Cutting-Edge Technology--DNA Microarrays (Gene Chips) | p. 461 |
Chapter 21 Construction and Screening of Human Antibody Libraries: Using State-of-the-Art Technology--Phage Display | p. 471 |
Chapter 22 Down-Regulation of Gene Expression in Mammalian Systems via Current siRNA Technology | p. 493 |
Index | p. 509 |