Available:*
Library | Item Barcode | Call Number | Material Type | Item Category 1 | Status |
|---|---|---|---|---|---|
Searching... | 30000010303798 | R895 C45 2012 | Open Access Book | Book | Searching... |
On Order
Summary
Summary
The imaging of small cellular components requires powerful instruments, and an entire family of equipment and techniques based on the confocal principle has been developed over the past 30 years. Such methods are commonly used by neuroscience researchers, but the majority of these users do not have a microscopy or a cell biology backgrounds and do can encounter difficulties in obtaining and interpreting results. This volume brings experts in high-resolution optical microscopy applications in neuroscience and cell biology together to document the state of the art. Outlining what is currently possible, the volume also discusses promising developments for the future and aids readers in selecting the most scientifically meaningful approach to solve their questions. Each chapter discusses instrumentation and technology in relationship to application in research. All of the common and cutting edge trends are covered - fluorescence / laser electron / nonlinear microscopy, infrared fluorescence, multiphoton imaging, tomography, FRAP, live imaging, STED, PALM/STORM, etc.
Table of Contents
| 1 Confocal laser scanning: Of instrument, computer processing, and menJeroen A.M. Beliën and Floris G. Wouterlood |
| 2 Beyond Abbe's resolution barrier: STED microscopyU. Valentin Nägerl |
| 3 Enhancement of optical resolution by 4pi single and multiphoton confocal fluorescence microscopyW.A. van Cappellen and A. Nigg and A.B. Houtsmuller |
| 4 Nano resolution optical imaging through localization microscopyHelge Ewers |
| 5 Optical investigation of brain networks using structured illuminationMarco Dal Maschio and Francesco Difato and Riccardo Beltramo and Angela Michela De Stasi and Axel Blau and Tommaso Fellin |
| 6 Multiphoton microscopy advances toward super resolutionPaolo Bianchini and Partha P. Mondal and Shilpa Dilipkumar and Francesca Cella Zanacchi and Emiliano Ronzitti and Alberto Diaspro |
| 7 The cell at molecular resolution: Principles and applications of cryo-electron tomographyRubén Fernández-Busnadiego and Vladan Lucic |
| 8 Cellular-level optical biopsy using full-field optical coherence microscopyArnaud Dubois |
| 9 Retroviral labeling and imaging of newborn neurons in the adult brainKurt A. Sailor and Hongjun Song and Guo-Li Ming |
| 10 Study of myelin sheaths by CARS microscopyChun-Rui Hu and Bing Hu and Ji-Xen Cheng |
| 11 High-resolution approaches to studying presynaptic vesicle dynamics using variants of FRAP and electron microscopyKevin Staras and Tiago Branco |
