Ei-Ichiro OchiaiThomas L. Poulos and Roger E. FennaCurtis W. Hoganson and Gerald T. BabcockLars Thelander and Astrid GraslundGeorg Auling and Hartmut FollmannWilliam L. Smith and Lawrence J. MarnettMaris G. N. HartmanisTetsuo TorayaEi-Ichiro OchiaiKenny K. Wong and John W. KozarichJames W. WhittakerPeter F. Knowles and David M. DooleyNigel L. Brown and Barry T. O. Lee and Simon Silver

Preface to the Series	p. iii
Preface to Volume 30	p. v
Contributors	p. xiii
Contents of Previous Volumes	p. xv
Handbook on Toxicity of Inorganic Compounds	p. xxxii
Handbook on Metals in Clinical and Analytical Chemistry	p. xxxiii
Chapter 1 Free Radicals and Metalloenzymes: General Considerations	p. 1
1. Introduction: A Survey of Biologically Relevant Free Radicals	p. 2
2. Necessity of Free Radicals and Metal Ions	p. 4
3. Reactivities of Free Radicals	p. 7
4. Summary of Free Radical-Dependent Metalloenzymes	p. 11
Abbreviations	p. 21
References	p. 22
Chapter 2 Peroxidases: Structure, Function, and Engineering	p. 25
1. Introduction	p. 26
2. Peroxidase Crystal Structures	p. 30
3. Peroxidase Catalytic Mechanisms	p. 48
4. Peroxidase Engineering	p. 56
5. Conclusions	p. 65
Abbreviations	p. 66
References	p. 67
Chapter 3 Photosystem II	p. 77
1. Introduction	p. 78
2. Identification and Spectroscopic Studies of the Tyrosine Radicals	p. 83
3. Electron Transfer Reactions of the Tyrosine Radicals--Both O[subscript 2]-Evolving and Inhibited Photosystem II	p. 89
4. Other Radicals in Photosystem II	p. 95
5. Concluding Remarks	p. 99
Abbreviations	p. 100
References	p. 100
Chapter 4 Ribonucleotide Reductase in Mammalian Systems	p. 109
1. Introduction	p. 110
2. Mammalian Ribonucleotide Reductase	p. 110
3. Herpes Simplex Virus Ribonucleotide Reductase	p. 115
4. Comparison of Amino Acid Sequences and Spectroscopic Properties for Different Species	p. 116
5. Formation, Properties, and Stability of the Fe-Tyrosyl Radical Center	p. 120
6. Inhibitors; Radical Scavengers; Iron Chelators; Specific Peptides	p. 121
7. Subunit Interaction and Electron Transfer	p. 124
Abbreviations	p. 125
References	p. 125
Chapter 5 Manganese-Dependent Ribonucleotide Reduction and Overproduction of Nucleotides in Coryneform Bacteria	p. 131
1. Introduction: The Diversity of Ribonucleotide Reductase Systems	p. 132
2. Effects of Manganese Depletion in Coryneform Bacteria	p. 136
3. The Manganese-Containing Ribonucleotide Reductase of Brevibacterium ammoniagenes	p. 142
4. Distribution of Manganese Ribonucleotide Reductases	p. 153
5. Concluding Remarks--Ribonucleotide Reduction: Universal but Not Uniform	p. 156
Abbreviations	p. 158
References	p. 158
Chapter 6 Prostaglandin Endoperoxide Synthases	p. 163
1. Introduction	p. 164
2. PGH Synthase Isozymes	p. 167
3. Cyclooxygenase Catalysis	p. 171
4. Peroxidase Catalysis	p. 179
5. Cyclooxygenase-Peroxidase Interrelationships	p. 184
6. PGH Synthase Structure	p. 186
Abbreviations	p. 192
References	p. 192
Chapter 7 Diol Dehydrase from Clostridium Glycolicum: The Non-B[subscript 12]-Dependent Enzyme	p. 201
1. Introduction	p. 202
2. Diol Metabolism in C. glycolicum	p. 203
3. Properties of Membrane bound Diol Dehydrase from C. glycolicum	p. 206
4. Characterization of Diol Dehydrase	p. 209
5. Conclusions	p. 213
Abbreviations	p. 214
References	p. 214
Chapter 8 Diol Dehydrase and Glycerol Dehydrase, Coenzyme B[subscript 12]-Dependent Isozymes	p. 217
1. Introduction	p. 218
2. Purification, Properties, and Structures	p. 219
3. Catalytic Properties and Mechanism of Action	p. 223
4. Formation of Free Radicals	p. 232
5. Inactivation and Reactivation	p. 240
6. Physiological Roles	p. 244
7. Concluding Remarks	p. 247
Abbreviations	p. 248
References	p. 249
Chapter 9 Adenosylcobalamin (Vitamin B[subscript 12] Coenzyme)-Dependent Enzymes	p. 255
1. Survey of Adenosylcobalamin-Dependent Enzymes	p. 256
2. Mechanisms of the Co-C Bond Cleavage	p. 259
3. Mechanisms of the Subsequent Reactions	p. 269
4. Conclusion	p. 275
Abbreviations	p. 275
References	p. 276
Chapter 10 S-Adenosylmethionine-Dependent Radical Formation in Anaerobic Systems	p. 279
1. Introduction	p. 280
2. Pyruvate Formate Lyase-Activating Enzyme	p. 280
3. Lysine 2,3-Aminomutase	p. 300
4. Anaerobic Ribonucleotide Reductase	p. 306
5. Concluding Remarks	p. 308
Abbreviations	p. 309
References	p. 309
Chapter 11 The Free Radical-Coupled Copper Active Site of Galactose Oxidase	p. 315
1. Introduction	p. 316
2. Historical Survey	p. 318
3. Recent Developments	p. 322
4. Model Reactions and the Role of the Radical in Catalysis	p. 352
Abbreviations and Definitions	p. 356
References	p. 356
Chapter 12 Amine Oxidases	p. 361
1. Introduction	p. 362
2. Structural Properties of Amine Oxidases	p. 365
3. Catalytic Mechanism of Amine Oxidases	p. 380
4. Biological Roles of Amine Oxidases	p. 392
5. Conclusions and Prospects	p. 395
Abbreviations	p. 396
References	p. 397
Chapter 13 Bacterial Transport of and Resistance to Copper	p. 405
1. Introduction	p. 406
2. Copper in Bacterial Nutrition	p. 407
3. Copper Transport and Uptake	p. 409
4. Bacterial Systems Conferring Resistance to Excess Copper	p. 415
5. Concluding Remarks	p. 428
Definitions and Abbreviations	p. 429
References	p. 430
Author Index	p. 435
Subject Index	p. 469