Regulation of gene expression by small RNAs

Endogenous small RNAs have been found in various organisms, including humans, mice, flies, worms, fungi, and bacteria. Furthermore, it's been shown that microRNAs acting as cellular rheostats have the ability to modulate gene expression. In higher eukaryotes, microRNAs may regulate as much as 50 percent of gene expression.

Regulation of Gene Expression by Small RNAs brings together the pioneering work of researchers who discuss their work involving a wide variety of small RNA regulatory pathways in organisms ranging from bacteria to humans. In addition to exploring the biogenesis and processing of these regulatory RNAs, they also consider the functional importance of these pathways in host organisms. Assisting current and future researchers, this unique groundbreaking work --

Provides a suite of cutting-edge resources for the study of microRNA ontology and function Includes a technology guide for those seeking to assay microRNA expression Explores the mechanisms by which microRNAs regulate gene expression in animal cells, including the regulation of gene expression by RNA-mediated transcriptional gene silencing Discusses a fast and low-cost approach for reversing genetic influences in mammals Looks at breakthroughs in the use of microRNA-based therapy for HIV and cancer

This volume captures the essence of the breadth and excitement surrounding the newly discovered regulatory roles of small RNAs. The powerful new approach in the study of gene function described in this text is leading to some remarkable findings that have the potential to revolutionize our understanding of genetic function and the treatment of diseases otherwise considered intractable.

Adam GrundhoffOla Snove and Pal SaetromPraveen Sethupathy and Molly Megraw and Artemis G. HatzigeorgiouHiroji AibaYang Yu and Timothy W. NilsenMona J. Nolde and Frank J. SlackChisato Ushida and Yusuke HokiiUtpal Bhadra and Sunit Kumar Singh and Pushpavalli and Praveensingh B. Hajeri and Manika Pal-BhadraAlex S. Flynt and Elizabeth J. Thatcher and James G. PattonZoltan HaveldaManu Agarwal and Julien Curaba and Xuemei ChenMirco Castoldi and Vladimir Benes and Martina U. MuckenthalerLori A. NeelyRajesh K. GaurShi-Lung Lin and Shao-Yao YingAnne B. Vojtek and Kwan-Ho Chung and Paresh D. Patel and David L. TurnerLinghua Qiu and Zuoshang XuYoichi R. FujiiSumedha D. JayasenaDaniel H. Kim and John J. RossiKevin V. Morris

Preface	p. ix
Acknowledgments	p. xi
About the Editors	p. xiii
Contributors	p. xv
Chapter 1 MicroMining: Computational Approaches to microRNA Discovery	p. 1
Chapter 2 Animal microRNA Gene Prediction	p. 21
Chapter 3 A Suite of Resources for the Study of microRNA Ontology and Function	p. 45
Chapter 4 Regulation of Translation and mRNA Stability by Hfq-Binding Small RNAs in Escherichia coli	p. 57
Chapter 5 Mechanisms by which microRNAs Regulate Gene Expression in Animal Cells	p. 77
Chapter 6 The microRNAs of Caenorhabditis elegans	p. 89
Chapter 7 Isolation and Characterization of Small RNAs in Caenorhabditis elegans	p. 101
Chapter 8 MicroRNA Tales in Fly Development	p. 123
Chapter 9 RNA Interference and microRNAs in Zebra Fish	p. 149
Chapter 10 Biogenesis and Function of Plant microRNAs	p. 173
Chapter 11 Endogenous Small RNA Pathways in Arabidopsis	p. 197
Chapter 12 How to Assay microRNA Expression: A Technology Guide	p. 215
Chapter 13 Methods to Quantify microRNA Gene Expression	p. 241
Chapter 14 Regulation of Alternative Splicing by microRNAs	p. 259
Chapter 15 Recent Progress in Polymerase II-Mediated Intronic microRNA Expression Systems	p. 275
Chapter 16 MicroRNA-Based RNA Polymerase II Expression Vectors for RNA Interference in Mammalian Cells	p. 301
Chapter 17 Transgenic RNAi: A Fast and Low-Cost Approach to Reverse Genetics in Mammals	p. 317
Chapter 18 Symphony of AIDS: A microRNA-Based Therapy	p. 333
Chapter 19 MicroRNAs and Cancer: Connecting the Dots	p. 351
Chapter 20 Mammalian Transcriptional Gene Silencing by Small RNAs	p. 393
Chapter 21 Regulation of Gene Expression by RNA-Mediated Transcriptional Gene Silencing	p. 405
Index	p. 419

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