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Cover image for Nonisotopic probing, blotting, and sequencing
Title:
Nonisotopic probing, blotting, and sequencing
Publication Information:
San Diego, CA : Academic Press, 1995
ISBN:
9780124262928

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30000010151884 QP519.9.M64 N66 1995 Open Access Book Book
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Summary

Summary

Since the publication of Nonistopic DNA Probe Techniques in 1992, the move away from radioactive materials for research and diagnostics has continued. This is due in part to public awareness of the hazards of radioactive waste and laws making radioactive disposal more difficult and costly and to improvement in both the sensitivity and convenience of nonisotopic techniques. Several new nonisotopic techniques have been developed and substantial improvements made to existing nonisotopic methods since 1992, and these are now included in Nonisotopic Probing, Blotting, and Sequencing .

Nonisotopic Probing, Blotting, and Sequencing is an updated, expanded edition of the bestseller, Nonisotopic DNA Probe Techniques . It has been thoroughly revised to include the latest improvements in nonisotopic tagging techniques for macromolecules. Like its predecessor, it enables researchers to select the best nonisotopic method for their needs and maximize success by following its straightforward protocols.


Table of Contents

Labels, Labelling, Analytical Strategies and Applications
Nonradioactive Labelling Methods for Nucleic Acids
Detection of Alkaline Phosphatase Labels By Time-Resolved Fluorescence
Detection of Alkaline Phosphatase By Bioluminescence
Detection of DNA on Membranes with Alkaline Phosphatase Labelled Probes and Chemiluminescence Cspdr Substrate
Detection of Alkaline Phosphatase By Colorimetry
Detection of Alkaline Phosphatase By Chemiluminescence Using NADP, Ascorbate and Indoxyl Phosphates
Detection of Horseradish Peroxidase By Enhanced Chemiluminescence
Dtection of Horseradish Peroxidase By Colorimetry
Detection of Glucose 6-Phosphate Dehydrogenase By Bioluminescence
Detection of Xanthine Oxidase By Enhanced Chemiluminescence
Electrochemiluminescent Detection of PCR-Derived Nucleic Acids
Detection of Phosphors By Phosphorescence
Detection of Europium Cryptates By Fluorescence
Detection of Lanthanid Chelates By Time-Resolved Fluorescence
Detection of Lanthanide Chelates and Multiple Labelling Strategies Based on Time-Resolved Fluorescence
Detection of Acridinium Esters By Chemiluminescence
Detection of Energy Transfer and Fluorescence Quenching
DNA Sequencing By Nonisotopic Methods
Chemiluminescent DNA Sequencing with 1, 2-Dioxetanes
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