Coherent Raman scattering microscopy

Compared to conventional Raman microscopy, coherent Raman scattering (CRS) allows label-free imaging of living cells and tissues at video rate by enhancing the weak Raman signal through nonlinear excitation. Edited by pioneers in the field and with contributions from a distinguished team of experts, Coherent Raman Scattering Microscopy explains how CRS can be used to obtain a point-by-point chemical map of live cells and tissues.

In color throughout, the book starts by establishing the foundation of CRS microscopy. It discusses the principles of nonlinear optical spectroscopy, particularly coherent Raman spectroscopy, and presents the theories of contrast mechanisms pertinent to CRS microscopy. The text then provides important technical aspects of CRS microscopy, including microscope construction, detection schemes, and data analyses. It concludes with a survey of applications that demonstrate how CRS microscopy has become a valuable tool in biomedicine.

Due to its label-free, noninvasive examinations of living cells and organisms, CRS microscopy has opened up exciting prospects in biology and medicine--from the mapping of 3D distributions of small drug molecules to identifying tumors in tissues. An in-depth exploration of the theories, technology, and applications, this book shows how CRS microscopy has impacted human health and will deepen our understanding of life processes in the future.

Author Notes

Ji-Xin Cheng is a professor of biomedical engineering at Purdue University. He earned a PhD from the University of Science and Technology of China and completed postdoctoral research at the Hong Kong University of Science and Technology and Harvard University. He is a pioneer in the development and biomedical application of molecular vibration-based imaging tools.

Xiaoliang Sunney Xie is a Mallinckrodt Professor of Chemistry at Harvard University. He earned a PhD from the University of California-San Diego and completed postdoctoral research at the University of Chicago. He is well-known for his innovations in nonlinear Raman microscopy and his pioneering work in single-molecule biophysical chemistry, including enzyme dynamics and live cell gene expression.

Eric Olaf Potma and Shaul MukamelEric Olaf Potma and Xiaoliang Sunney Xie and Andreas Volkmer and Ji-Xin ChengBrian G. Saar and Xiaoliang Sunney XieChristian Freudiger and Xiaoliang Sunney XieMikhail N. Slipchenko and Delong Zhang and Ji-Xin ChengGangjun Liu and Mihaela Balu and Zhongping Chen and Eric Olaf PotmaAlexander Jesacher and GregorThalhammer and Stefan Bernet and Monika Ritsch-MarteMikhail N. Slipchenko and Ji-Xin ChengJonathan M. Levitt and Ori Katz and Yaron SilberbergJennifer P. OgilvieVarun Raghunathan and Hyunmin Kim and Stephan Stranick and Eric Olaf PotmaMartin Jurna and Cees Otto and Herman L. OfferhausJames P.R. Day and Katrin F. Domke and Gianluca Rago and Erik M. Vartiainen and Mischa BonnSang-Hyun LimMarcus T. Cicerone and Young Jong Lee and Sapun H. Parekh and Khaled A. AamerDan Fu and Xiaoliang Sunney XieVan Fu. Yunzhou (Sophia) Shi. and Ji-Xin ChengHelen Fink and Christian Brackmann and Annika EnejderThuc T. LeErik Bélanger and F.P. Henry and R. Vallée and M.A. Randolph and I.E. Kochevar and J.M. Winograd and Charles P. Lin and Daniel CôtéHeung-Shik Park and Oleg D. LavrentovichHideaki KanoLing Tong and Ji-Xin ChengChristian Freudiger and Daniel A. Orringer and Xiaoliang Sunney XieHan-Wei Wang and Michael Sturek and Ji-Xin ChengAnnika Enejder and Christian BrackmannKimberly K. Buhman

Series Preface	p. ix
Preface	p. xi
Editors	p. xv
Contributors	p. xvii
Part I Theory
1 Theory of Coherent Raman Scattering	p. 3
2 Coherent Raman Scattering under Tightly Focused Conditions	p. 43
Part II Platforms
3 Construction of a Coherent Raman Microscope	p. 79
4 Stimulated Raman Scattering Microscopy	p. 99
5 Femtosecond versus Picosecond Pulses for Coherent Raman Microscopy	p. 121
6 Miniature Coherent Raman Probes for In Vivo Biomedical Imaging	p. 137
7 Wide-Field CARS-Microscopy	p. 161
8 Vibrational Spectromicroscopy by Coupling Coherent Raman Imaging with Spontaneous Raman Spectral Analysis	p. 189
9 Coherent Control in CARS	p. 207
10 Fourier Transform CARS Microscopy	p. 237
11 CRS with Alternative Beam Profiles	p. 253
12 Vibrational Phase Microscopy	p. 277
13 Multiplex CARS Microscopy	p. 291
14 Interferometric Multiplex CARS	p. 309
15 Photonic Crystal Fiber-Based Broadband CARS Microscopy	p. 329
16 Multiplex Stimulated Raman Scattering Microscopy	p. 353
Part III Applications
17 Imaging Myelin Sheath Ex Vivo and In Vivo by CARS Microscopy	p. 389
18 Imaging Lipid Metabolism in Caenorhabditis elegans and Other Model Organisms	p. 405
19 Lipid-Droplet Biology and Obesity-Related Health Risks	p. 421
20 White Matter Injury: Cellular-Level Myelin Damage Quantification in Live Animals	p. 439
21 CARS Microscopy Study of Liquid Crystals	p. 457
22 Live Cell Imaging by Multiplex CARS Microspectroscopy	p. 479
23 Coherent Raman Scattering Imaging of Drug Delivery Systems	p. 505
24 Applications of Stimulated Raman Scattering Microscopy	p. 519
25 Applications of Coherent Anti-Stokes Raman Spectroscopy Imaging to Cardiovascular Diseases	p. 537
26 Applications of CARS Microscopy to Tissue Engineering	p. 547
27 Dietary Fat Absorption Visualized by CARS Microscopy	p. 569
Index	p. 581

Available:*

On Order

Summary

Summary

Author Notes

Table of Contents