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Summary
Summary
Driven in part by the development of genomics, proteomics, and bioinformatics as new disciplines, there has been a tremendous resurgence of interest in physical methods to investigate macromolecular structure and function in the context of living cells. This volume in Methods in Cell Biology is devoted to biophysical techniques in vivo and their applications to cellular biology. Biophysical Tools for Biologists covers methods-oriented chapters on fundamental as well as cutting-edge techniques in molecular and cellular biophysics. This book is directed toward the broad audience of cell biologists, biophysicists, pharmacologists, and molecular biologists who employ classical and modern biophysical technologies or wish to expand their expertise to include such approaches. It will also interest the biomedical and biotechnology communities for biophysical characterization of drug formulations prior to FDA approval.
Author Notes
Professor of Biochemistry and Marine Biology at Northeastern University, promoted 1996. Joined Northeastern faculty in 1987. Previously a faculty member in Dept. of Biochemistry at the University of Mississippi Medical Center, 1983-1987.Principal Investigator in the U.S. Antarctic Program since 1984. Twelve field seasons "on the ice" since 1981. Research conducted at Palmer Station, Antarctica, and McMurdo Station, Antarctica.Research areas: Biochemical, cellular, and physiological adaptation to low and high temperatures. Structure and function of cytoplasmic microtubules and microtubule-dependent motors from cold-adapted Antarctic fishes. Regulation of tubulin and globin gene expression in zebrafish and Antarctic fishes. Role of microtubules in morphogenesis of the zebrafish embryo. Developmental hemapoiesis in zebrafish and Antarctic fishes. UV-induced DNA damage and repair in Antarctic marine organisms.
Table of Contents
| Section 1 Fluorescence Methods |
| 1 Photoactivation and Photobleaching Techniques for Analysis of Organelle Biogenesis in vivo |
| 2 Analysis of the Dynamics of Living Cells by Fluorescence Correlation Spectroscopy |
| 3 Molecular Sensors Based on Fluorescence Resonance Energy Transfer to Visualize Cellular Dynamics |
| 4 Real-Time Fluorescence of Protein Folding in vivo |
| 5 Microfluidic Glucose Stimulation of Ca+2 Oscillations in Pancreatic Islets |
| Section 2 Microscopic Methods |
| 6 Introduction to Optical Sectioning: Confocal, Deconvolution, and Two-Photon |
| 7 Use of Electron Tomography to Elucidate Sub-Cellular Structure and Function |
| 8 Proteomics of Macromolecular Complexes by Cellular Cryo-Electron Tomography |
| 9 Total Internal Reflectance Microscopy (TIRF) |
| 10 Atomic Force Microscopy of Living Cells |
| 11 Real-Time Kinetics of Gene Activity in Individual Bacteria |
| 12 Measurement of Cytoskeletal Proteins Globally and Locally in vivo |
| 13 Infrared and Raman Microscopy in Cell Biology |
| 14 Imaging Fluorescent Mice in vivo by Confocal Microscopy |
| 15 Nanoscale Imaging of Intracellular Fluorescent Proteins: Breaking the Diffraction Barrier |
| Section 3 Methods at the In Vitro/In Vivo Interface |
| 16 Analysis of Protein Posttranslational Modification by Mass Spectrometry |
| 17 Imaging Mass Spectrometry |
| 18 Wet EM Using Quantum Dots |
| 19 Single Cell Capillary Electrophoresis |
| Section 4 Methods for Diffusion, Viscosity, Force and Displacement |
| 20 Single-Molecule Force Spectroscopy in Living Cells |
| 21 Magnetic Bead Force Applications |
| 22 Measurement of Membrane-Cytoskeleton Adhesion Using Laser Optical Tweezers |
| 23 Cellular Rheological Measurements in vivo |
| 24 Physical Behavior of Cytoskeletal Networks in vitro and in vivo |
| 25 Force Regulation of Microtubule Dynamics in Fission Yeast |
| Section 5 Techniques for Protein Activity, Protein-Protein and Protein-RNA Interactions |
| 26 Quantifying Protein Activity Using FRET and FLIM Microscopy |
| 27 Measurement of Protein-Protein Interactions in vivo Using FRET and FLIM |
| 28 Measurement of RNA Interactions in vivo Using Molecular Beacons |
| Section 6 Computational Modeling |
| 29 Stochastic Modeling in Cell Biology |
| 30 Computational Methods for Analyzing Patterns in Dynamic Biological Phenomena: An Application to Microtubule Dynamics |
| 31 Computational Modeling of Self-Organized Spindle Formation |
