Hans von Dohren and Wibke Kallow and Mary Anne Tavanlar and Torsten Schwecke and Ralf Dieckmann and Volker UhlmannJoe E. Dotzlaf and Steven W. Queener and Wu-Kuang YehArnold L. Demain and Jose L. Adrio and Jacqueline M. PiretAkira Arisawa and Hiroshi TsunekawaClaudio D. Denoya and Kim J. Stutzman-Engwall and Hamish A. I. McArthurRamesh N. PatelJon D. Stewart and Sonia Rodriguez and Margaret M. KayserMichael D. GrimAndrew R. Cockshott and Adam J. Kreuzman and Wu-Kuang YehSiddhartha RoychoudhuryGenshi Zhao and Timothy I. Meier and Wu-Kuang YehMichele C. Smith and James A. Cook and Gary M. Birch and Stephen A. Hitchcock and Robert B. Peery and Joann Hoskins and Paul L. Skatrud and Raymond C. Yao and Karen L. CoxQ. May Wang and Robert B. JohnsonTimothy G. GearyHiroshi Tomoda and Satoshi OmuraHsiu-Chiung Yang and Marian Mosior and Edward A. DennisRobert McDaniel and Chaitan KhoslaKira J. Weissman and James StauntonMirko Glinski and Till Hornbogen and Rainer ZocherJoaquim Trias and Zhengyu YuanMolly B. SchmidRidong ChenRidong ChenSrinivasan Krishnan and John E. Hale and Gerald W. Becker

Preface	p. iii
Contributors	p. xi
I. Biosynthesis
1. [delta]-(L-[alpha]-Aminoadipyl)-L-Cysteinyl-D-Valine Synthetase as a Model Tripeptide Synthetase	p. 1
2. Metabolic Engineering for Cephalosporin C Yield Improvement and Production of Intermediates	p. 39
3. Bioconversion of Penicillins to Cephalosporins	p. 61
4. Direct Fermentative Production of Acyltylosins by Genetically Engineered Strains of Streptomyces fradiae	p. 89
5. Engineering Streptomyces avermitilis for the Production of Novel Avermectins: Mutant Design and Titer Improvement	p. 113
II. Biocatalysis
6. Biocatalytic Syntheses of Chiral Intermediates for Antihypertensive Drugs	p. 137
7. Cloning, Structure, and Activity of Ketone Reductases from Baker's Yeast	p. 175
8. Cross-Linked Enzyme Crystals: Biocatalysts for the Organic Chemist	p. 209
9. Enzymatic Deacylation of Echinocandins and Related Antifungal Agents	p. 227
III. Screening/Optimization
10. Roles of Enzymes in Antibacterial Drug Discovery	p. 245
11. Penicillin-Binding Proteins as Antimicrobial Targets: Expression, Purification, and Assay Technologies	p. 263
12. Development of a High-Throughput Screen for Streptococcus pneumoniae UDP-N-Acetylmuramoyl-Alanine: D-Glutamate Ligase (MurD) for the Identification of MurD Inhibitors	p. 289
13. Purification and Assay Development for Human Rhinovirus Proteases	p. 307
14. Screening for Parasiticides Using Recombinant Microorganisms	p. 323
15. Screening for Inhibitors of Lipid Metabolism	p. 343
16. Design and Development of a Selective Assay System for the Phospholipase A[subscript 2] Superfamily	p. 379
IV. Emerging Technologies
17. Understanding and Exploiting Bacterial Polyketide Synthases	p. 397
18. Polyketide Synthases: Analysis and Use in Synthesis	p. 427
19. Enzymatic Synthesis of Fungal N-Methylated Cyclopeptides and Depsipeptides	p. 471
20. New Strategies for Target Identification, Validation, and Use of Enzymes in High-Throughput Screening	p. 499
21. Use of Genomics for Enzyme-Based Drug Discovery	p. 515
22. Assigning Precise Function to Genes	p. 537
23. Redesigning Binding and Catalytic Specificities of Enzymes	p. 555
24. Proteomics: Chromatographic Fractionation Prior to Two-Dimensional Polyacrylamide Gel Electrophoresis for Enrichment of Low-Abundance Proteins to Facilitate Identification by Mass Spectrometric Methods	p. 575
Index	p. 597