Plant genomics : methods and protocols

Plant genomics is a growing and constantly evolving field of study, one which has gained much ground in past years through the development of advanced research and data management tools. In Plant Genomics: Methods and Protocols, expert researchers explore the current issues and methodologies of this expanding field, specifically addressing areas of gene discovery and the functional analysis of genes with a focus on the primary tools and sub-disciplines of genetic mapping, mRNA, protein and metabolite profiling. Chapters employ exciting new methods to investigate molecular plant breeding technology and gene functional analysis via transformation, mutation, protein function, and gene expression. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Comprehensive and innovative, Plant Genomics: Methods and Protocols is an essential guide for all plant scientists who are interested in further studies in the area of genomics research.

Richard FlavellNikki Appleby and David Edwards and Jacqueline BatleyChris Duran and David Edwards and Jacqueline BatleyBu-Jun Shi and J. Perry Gustafson and Peter LangridgeElena Potokina and Arnis Druka and Michael J. KearseyChristof Rampitsch and Natalia V. BykovaHuw D. Jones and Caroline A. SparksHuw D. Jones and Angela Doherty and Caroline A. SparksStephen J. Robinson and Isobel A. P. ParkinNaser Farrokhi and Maria Hrmova and Rachel A. Burton and Geoffrey B. FincherMaria Hrmova and Geoffrey B. FincherSinéad Drea and Paul Derbyshire and Rachil Koumproglou and Liam Dolan and John H. Doonan and Peter ShawDavid E. Matthews and Gerard R. Lazo and Olin D. AndersonShu Ouyang and Françe;oise Thibaud-Nissen and Kevin L. Childs and Wei Zhu and C. Robin BuellRajeev K. Varshney and Dave A. Hoisington and Spurthi N. Nayak and Andreas GranerDavid A. Fischhoff and Molly N. ClineEviatar NevoMichael Imelfort and Jacqueline Batley and Sean Grimmond and David Edwards

Preface	p. v
Contributors	p. ix
1 Role of Model Plant Species	p. 1
2 New Technologies for Ultra-High Throughput Genotyping in Plants	p. 19
3 Genetic Maps and the Use of Synteny	p. 41
4 A Simple TAE-Based Method to Generate Large Insert BAC Libraries from Plant Species	p. 57
5 Transcript Profiling and Expression Level Mapping	p. 81
6 Methods for Functional Proteomic Analyses	p. 93
7 Stable Transformation of Plants	p. 111
8 Transient Transformation of Plants	p. 131
9 Bridging the Gene-to-Function Knowledge Gap Through Functional Genomics	p. 153
10 Heterologous and Cell-Free Protein Expression Systems	p. 175
11 Functional Genomics and Structural Biology in the Definition of Gene Function	p. 199
12 In situ Analysis of Gene Expression in Plants	p. 229
13 Plant and Crop Databases	p. 243
14 Plant Genome Annotation Methods	p. 263
15 Molecular Plant Breeding: Methodology and Achievements	p. 283
16 Practical Delivery of Genes to the Marketplace	p. 305
17 Ecological Genomics of Natural Plant Populations: The Israeli Perspective	p. 321
18 Genome Sequencing Approaches and Successes	p. 345
Index	p. 359

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Table of Contents