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Library | Item Barcode | Call Number | Material Type | Item Category 1 | Status |
|---|---|---|---|---|---|
Searching... | 30000010222738 | QL638.C94 E87 2009 | Open Access Book | Book | Searching... |
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Summary
Summary
Due to its prolific reproduction and the external development of the transparent embryo, the zebrafish is the prime model for genetic and developmental studies, as well as research in genomics. While genetically distant from humans, nonetheless the vertebrate zebrafish has comparable organs and tissues that make it the model organism for study of vertebrate development. This book, one of two new volumes in the Reliable Lab Solutions series dealing with zebrafish, brings together a robust and up-to-date collection of time-tested methods presented by the world's leading scientists. Culled from previously published chapters in Methods in Cell Biology and updated by the original authors where relevant, it provides a comprehensive collection of protocols describing the most widely used techniques relevant to the study of the cellular and developmental biology of zebrafish. The methods in this volume were hand-selected by the editors, whose goal was to a provide a handy and cost-effective collection of fail-safe methods, tips, and "tricks of the trade" to both experienced researchers and more junior members in the lab.
Author Notes
Professor, Department of Biology, Institute of Neuroscience, University of Oregon, Eugene, OR, USA
Professor of Biochemistry and Marine Biology at Northeastern University, promoted 1996. Joined Northeastern faculty in 1987. Previously a faculty member in Dept. of Biochemistry at the University of Mississippi Medical Center, 1983-1987.Principal Investigator in the U.S. Antarctic Program since 1984. Twelve field seasons "on the ice" since 1981. Research conducted at Palmer Station, Antarctica, and McMurdo Station, Antarctica.Research areas: Biochemical, cellular, and physiological adaptation to low and high temperatures. Structure and function of cytoplasmic microtubules and microtubule-dependent motors from cold-adapted Antarctic fishes. Regulation of tubulin and globin gene expression in zebrafish and Antarctic fishes. Role of microtubules in morphogenesis of the zebrafish embryo. Developmental hemapoiesis in zebrafish and Antarctic fishes. UV-induced DNA damage and repair in Antarctic marine organisms.
Table of Contents
| Overview of the Zebrafish SystemH. William |
| Detrich III Cell Cycles and Development in the Embryonic ZebrafishDonald A. Kane |
| Primary Fibroblast Cell CultureBarry H. Paw |
| Production of Haploid and Diploid Androgenetic Zebrafish (Including Methodology for Delayed in Vitro Fertilization)John H. Postlethwait |
| Analysis of Protein and Gene Expression Trevor Jowett Analysis of Zebrafish Development Using Explant Culture Assays Hazel Sive Confocal Microscopic Analysis of Morphogenetic MovementsMark S. Cooper |
| Cytoskeletal Dynamics of the Zebrafish Embryo Lilianna Solinica-Krezel Haploid Screens and Gamma-Ray Mutagenesis Charline Walker Positional Cloning of Mutated Zebrafish Genes Will Talbot The Zebrafish GenomeJohn Postlethwait |
| Analyzing Axon Guidance in the Zebrafish Retinotectal SystemChi-Bin Chien |
| Analysis of the Cell Cycle in Zebrafish EmbryosJames F. Amatruda |
| Cellular Dissection of Zebrafish HematopoiesisDavid Traver |
| Culture of Embryonic Stem Cell Lines from ZebrafishPaul Collodi |
| Neurogenesis Laure Bally-Cuif Time-Lapse Microscopy of Brain DevelopmentScott E. Fraser |
| Development of the Peripheral Sympathetic Nervous System in ZebrafishPaul D. Henion |
| Approaches to Study Neurogenesis in the ZebrafishRetina Jarema Malicki |
| Instrumentation for Measuring Oculomotor Performance and Plasticity in Larval OrganismsDavid W. Tank |
| Development of Cartilage and BoneThomas F. Schilling |
| Morphogenesis of the Jaw: Development Beyond the EmbryoPamela C. Yelick |
| Cardiac Development Didier Y. R. Stainier Zebrafish Kidney DevelopmentIain A. Drummond |
| Retroviral-Mediated Insertional Mutogenesis in ZebrafishAdam Amsterdam |
| Genetic Screens for Maternal-Effect MutationsFrancisco Pelegri |
| Behavior Screening Assays in ZebrafishHerwig Baier |
| A High-Throughput Method for Identifying N-Ethyl-N-Nitrosourea (ENU)-Induced Point Mutations in ZebrafishCecelia B. Moens |
| Transgenesis and Gene Trap Methods in Zebrafish by Using the Tol2 Transposable ElementKoichi Kawakami Molecular |
| Cytogenetic Methodologies and a Bacterial Artificial Chromosome (BAC)Probe Panel Resource for Genomic Analysis in ZebrafishCharles Lee |
| The Zon Laboratory Guide to Positional Cloning in ZebrafishLeonard I. Zon |
| Sleeping Beauty Transposon for Efficient Gene DeliveryStephen C. Ecker |
| Transgene Manipulation in Zebrafish by Using RecombinasesGary W. Stuart |
| Highly Efficient Zebrafish Transgenesis Mediated by the Meganuclease I-SceI Joachim Wittbrodt Cloning Zebrafish by Nuclear Transfer Shuo Lin Spatial and Temporal Expression of the Zebrafish Genome by Large-Scale |
| In Situ Hybridization Screening Christine Thisse Genetic Backgrounds, Standard Lines, and Husbandry of Zebrafish Bill Trevarrow Common Diseases of Laboratory ZebrafishJennifer L. Matthews |
| Zebrafish Sperm CryopreservationThomas Look |
