Cover image for Plant cell culture : essential methods
Title:
Plant cell culture : essential methods
Personal Author:
Davey, M. R. (Michael Raymond), 1944-
Publication Information:
Hoboken, NJ : Wiley-Blackwell, 2010
Physical Description:
xvi, 341 p. : ill. ; 25 cm.
ISBN:
9780470686485
Subject Term:
Plant cell culture

Plant tissue culture
Added Author:
Anthony, Paul

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 30000010236386 QK725 D38 2010 Open Access Book Book
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Summary

Summary

The ability to culture cells is fundamental for mass propagation and as a baseline for the genetic manipulation of plant nuclei and organelles. The introduction to Plant Cell Culture: Essential Methods provides a general background to plant cell culture, including basic principles, technologies and laboratory practices that underpin the more detailed techniques described in subsequent chapters. Whilst each chapter provides a background to the topic area and methodology, a crucial aspect is the provision of detailed protocols with emphasis on trouble shooting, describing common problems and detailed advice for their avoidance. Plant Cell Culture: Essential Methods provides the reader with a concise overview of these techniques, including micropropagation, mutagenesis, cryopreservation, genetic and plastid transformation and somatic cell technologies. This book will be an essential addition to any plant science laboratory's bookshelf. Highlights the best and most up-to-date techniques for working on plant cell culture Explains clearly and precisely how to carry out selected techniques in addition to background information on the various approaches Chapters are written by leading international authorities in the field and cover both well-known and new, tried and tested, methods for working in plant cell culture

An essential laboratory manual for students and early-career researchers.


Author Notes

Michael R. Davey and Paul Anthony are the authors of Plant Cell Culture: Essential Methods, published by Wiley.


Table of Contents

Preface
Contributors
1 Plant MicropropagationIvan Iliev and Alena Gajdosova and Gabriela Libiakova and Shri Mohan Jain
1.1 Introduction
1.2 Methods and approaches
1.2.1 Explants and their surface disinfection
1.2.2 Culture media and their preparation
1.2.3 Stages of micropropagation
1.2.4 Techniques of micropropagation
1.3 Troubleshooting
References
2 Thin Cell Layers: The TechniqueJaime A. Teixeira da Silva and Michio Tanaka
2.1 Introduction
2.2 Methods and approaches
2.2.1 TCL
2.2.2 Choice of material: Cymbidium hybrid
2.3 Troubleshooting
2.3.1 General comments
References
3 Plant Regeneration - Somatic EmbryogenesisKim E. Nolan and Ray J.Rose
3.1 Introduction
3.2 Methods and approaches
3.2.1 Selection of the cultivar and type of explant
3.2.2 Culture media
3.2.3 Preparation of culture media
3.2.4 Sterilization of tissues and sterile technique
3.2.5 Culture and growth of tissue
3.2.6 Culture and induction of somatic embryos
3.2.7 Embryo development
3.2.8 Transfer to soil - the final stage of regeneration
3.3 Troubleshooting
References
4 Haploid PlantsSant S. Bhojwani and Prem K.Dantu
4.1 Introduction
4.2 Methods and approaches
4.2.1 Androgenesis
4.2.2 Diploidization
4.3 Troubleshooting
References
5 Embryo RescueTraud Winkelmann and Antje Doil and Sandra Reinhardt and Aloma Ewald
5.1 Introduction
5.2 Methods and approaches
5.2.1 Identification of the time and type of barrier in hybridization
5.2.2 Isolation of plant material after fertilization
5.2.3 Culture conditions and media
5.2.4 Confirmation of hybridity and ploidy
5.2.5 Conditions for regeneration of embryos to plants
5.3 Troubleshooting
References
6 In vitro Flowering and Seed Set: Acceleration of Generation CyclesSergio J. Ochatt and Rajbir S.Sangwan
6.1 Introduction
6.2 Methods and approaches
6.2.1 Protein legumes [7]
6.2.2 Arabidopsis thaliana [13]
6.3 Troubleshooting
References
7 Induced Mutagenesis in Plants Using Physical and Chemical AgentsChikelu Mba and Rownak Afza and Souleymane Bado and Shri Mohan Jain
7.1 Introduction
7.2 Methods and approaches
7.2.1 Determination of the optimal doses of mutagens for inducing mutations
7.3 Troubleshooting
7.3.1 Factors influencing the outcome of mutagenesis using chemical mutagens
7.3.2 Factors influencing the outcome of mutagenesis using physical mutagens
7.3.3 Facts about induced mutations
References
8 Cryopreservation of Plant GermplasmE.R. Joachim Keller and Angelika Senula
8.1 Introduction
8.2 Methods and approaches
8.2.1 Main principles
8.2.2 Slow (two-step) freezing
8.2.3 Vitrification
8.2.4 Encapsulation-dehydration
8.2.5 DMSO droplet freezing
8.2.6 Combined methods
8.2.7 Freezing of cold-hardened buds
8.2.8 Freezing of orthodox seeds
8.2.9 Freezing of pollen and spores
8.3 Troubleshooting
References
9 Plant Protoplasts: Isolation, Culture and Plant RegenerationMichael R. Davey and Paul Anthony and Deval Patel and J. Brian Power
9.1 Introduction
9.2 Methods and approaches
9.2.1 Protoplast isolation
9.2.2 Protoplast culture
9.3 Troubleshooting
References
10 Protoplast Fusion Technology - Somatic Hybridization and CybridizationJude W. Grosser and Milica Calovic and Eliezer S.Louzada
10.1 Introduction
10.2 General applications of somatic hybridization
10.3 Methods and approaches
10.4 Troubleshooting
References
11 Genetic Transformation - AgrobacteriumIan S. Curtis
11.1 Introduction
11.2 Methods and approaches
11.2.1 Agrobacterium as a natural genetic engineer
11.2.2 Vector systems for transformation
11.2.3 Inoculation procedures
11.3 Troubleshooting
References
12 Genetic Transformation - BiolisticsFredy Altpeter and Sukhpreet Sandhu
12.1 Introduction
12.2 Methods and approaches
12.2.1 Biolistic technology
12.2.2 Optimization of gene delivery parameters
12.2.3 Target tissues
12.2.4 Reporter gene assays
12.2.5 Selection and plant regeneration
12.3 Troubleshooting
References
13 Plastid TransformationBridget V. Hogg and Cilia L.C. Lelivelt and Aisling Dunne and Kim-Hong Nguyen and Jacqueline M. Nugent
13.1 Introduction
13.2 Methods and approaches
13.2.1 Principles of plastid transformation
13.2.2 Biolistic-mediated plastid transformation
13.2.3 PEG-mediated plastid transformation
13.2.4 Identification and characterization of transplastomic plants
13.3 Troubleshooting
13.3.1 Biolistic-mediated transformation
13.3.2 PEG-mediated transformation
References
14 Molecular Characterization of Genetically Manipulated PlantsCristiano Lacorte and Giovanni Vianna and Francisco J.L. Aragao and Eĺibio L. Rech
14.1 Introduction
14.2 Methods and approaches
14.2.1 Plant DNA extraction
14.2.2 Polymerase chain reaction
14.2.3 Southern blot technique
14.2.4 Analysis of the integration site: inverse PCR (iPCR) and thermal asymmetric interlaced PCR (Tail-PCR)
14.3 Troubleshooting
References
15 BioreactorsSpiridon Kintzios
15.1 Introduction
15.2 Methods and approaches
15.2.1 Medium scale disposable or semidisposable airlift reactors
15.2.2 The RITA temporary immersion reactor
15.2.3 The LifeReactor
15.2.4 Immobilized cell bioreactors
15.2.5 Mini-bioreactors
15.3 Troubleshooting
References
16 Secondary ProductsKexuan Tang and Lei Zhang and Junfeng Chen and Ying Xiao and Wansheng Chen and Xiaofen Sun
16.1 Introduction
16.2 Methods and approaches
16.2.1 Plant cell cultures
16.2.2 Scale-up and regulation of secondary metabolite production
16.2.3 Detection of secondary products
16.3 Troubleshooting
References
17 Plant Cell Culture - Present and FutureJim M. Dunwell
17.1 Introduction
17.2 Micropropagation
17.3 Embryogenesis
17.3.1 Background
17.3.2 Commercial exploitation of somatic embryos
17.3.3 Molecular aspects of somatic embryogenesis
17.3.4 Microspore derived embryos
17.4 Haploid methodology
17.4.1 Haploids and their exploitation
17.4.2 Induction of haploid plants
17.4.3 Molecular aspects of haploid induction from microspores
17.4.4 Ab initio zygotic-like embryogenesis from microspores
17.5 Somaclonal variation
17.6 Transgenic methods
17.6.1 Background
17.6.2 Regeneration and transformation techniques
17.6.3 Chloroplast transformation
17.6.4 Biopharming
17.7 Protoplasts and somatic hybridization
17.8 Bioreactors
17.8.1 Production of plant products
17.8.2 Production of pharmaceuticals
17.8.3 Production of food ingredients
17.8.4 Production of cosmetics
17.8.5 Analytical methodology
17.9 Cryopreservation
17.10 Intellectual property and commercialization
17.10.1 Background
17.10.2 Sources of patent and other relevant information
17.11 Conclusion
References
Index