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Library | Item Barcode | Call Number | Material Type | Item Category 1 | Status |
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Searching... | 30000010231228 | QL121 M47 2010 | Open Access Book | Book | Searching... |
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Summary
Summary
For years scientists viewed the deep sea as calm, quiet, and undisturbed, with marine species existing in an ecologically stable and uniform environment. Recent discoveries have completely transformed that understanding and the deep sea is recognized as a complicated and dynamic environment with a rich diversity of marine species. Carefully designed to provide practical information in an easily accessible format, Methods for the Study of Deep-Sea Sediments, Their Functioning, and Biodiversity covers how to investigate the biological components through analysis of their biodiversity. It also provides the protocols and methodological details needed to investigate some aspects of the functional biodiversity of variables commonly utilized to describe and understand the drivers of deep-sea ecosystem functioning.
This volume contains detailed protocols for analyzing all benthic components from benthic viruses, prokaryotes, protozoa, foraminifera, to meio-, macro-, and megafauna. It includes step-by-step procedures, with additional notes on the crucial steps or possible difficulties arising from the analysis. Each chapter provides a brief introduction, a description of the sampling procedures and/or the sample treatment, and then the laboratory protocols, providing information on instrument setting and/or the solutions utilized. Each chapter also contains a visual scheme of the protocol for use during laboratory activities and for tracking each laboratory step. Linking information on biodiversity with the functioning of the marine ecosystems, the book covers all living components of the benthos. It provides practical information for anyone studying deep-sea habitats, their characteristics, functioning, and biodiversity.
Author Notes
Director Department of Marine Sciences, Polytechnic University of Marche, Ancona, Italy
Table of Contents
Foreword | p. xi |
Premise | p. xiii |
Preface | p. xv |
Contributors | p. xvii |
Introduction | p. xix |
Section I Deep-Sea Environmental Variables | |
Chapter 1 Total Organic Matter and Water Contents, Grain Size, Bulk Density, Porosity, and Redox Potential of Sediments | p. 3 |
Chapter 2 Total Organic Carbon, Total Nitrogen, and Organic Phosphorus in Marine Sediments | p. 13 |
Chapter 3 Bioavailable Organic Matter: Total and Enzymatically Hydrolyzable Proteins, Carbohydrates, and Lipids | p. 23 |
Chapter 4 Photosynthetic Pigment Concentrations in Marine Sediments | p. 45 |
Section II Food Supply of Organic Matter to the Deep-Sea Floor | |
Chapter 5 Fluxes of Labile Organic Matter to the Sea Floor | p. 55 |
Chapter 6 Total Carbohydrate Flux from Sediment Trap Samples | p. 59 |
Chapter 7 Total Protein Flux from Sediment Trap Samples | p. 63 |
Chapter 8 Total Lipid Flux from Sediment Trap Samples | p. 69 |
Chapter 9 Total DNA from Sediment Trap Samples | p. 75 |
Chapter 10 Phytopigment Flux from Sediment Trap Samples | p. 81 |
Section III Deep-Sea Benthic Life | |
Chapter 11 Viral Abundance | p. 87 |
Chapter 12 Prokaryotic Abundance | p. 95 |
Chapter 13 Fluorescence In Situ Hybridization and Catalyzed Reporter Deposition for Benthic Prokaryote Assemblage Structure | p. 107 |
Chapter 14 Prokaryotic Abundance by Real-Time PCR | p. 121 |
Chapter 15 Abundance of Heterotrophic Benthic Protists | p. 131 |
Chapter 16 Abundance of Benthic Foraminitera | p. 141 |
Chapter 17 Abundance of Metazoan Meiofauna | p. 149 |
Chapter 18 Macrofaunal Abundance | p. 161 |
Chapter 19 Megafaunal Abundance | p. 167 |
Section IV Deep-Sea Benthic Diversity | |
Chapter 20 Extraction and Purification of DNA from Marine Sediments Suitable for the Analysis of Prokaryotic Diversity | p. 181 |
Chapter 21 Archaeal Diversity Analysis Using 16S rDNA T-RFLP (Terminal-Restriction Fragment Length Polymorphisms) | p. 197 |
Chapter 22 Benthic Bacterial Diversity Based on Cloning and Sequencing of 16S rRNA Genes | p. 209 |
Chapter 23 Benthic Bacterial Diversity Analysis Based on ARISA (Automated Ribosomal Intergenic Spacer Analysis) | p. 223 |
Chapter 24 Meiofaunal Diversity | p. 235 |
Chapter 25 Macrofaunal and Megafaunal Diversity | p. 253 |
Chapter 26 Parameters for the Measurement of Marine Benthic Structural and Functional Diversity | p. 259 |
Section V Deep-Sea Benthic Functioning | |
Chapter 27 Deep-Sea Experiments and Manipulations | p. 269 |
Chapter 28 Effect of Pressure on Enzymatic Activities, Viral Production, and Prokaryotic Heterotrophic Production | p. 285 |
Chapter 29 Organic Carbon Remineralization Rates in Marine Surface Sediments | p. 291 |
Chapter 30 Degradation and Turnover of Organic Matter in Marine Sediments | p. 297 |
Chapter 31 Extracellular DNA Extraction from Marine Sediments | p. 303 |
Chapter 32 Degradation of Extracellular DNA in Marine Sediments | p. 311 |
Chapter 33 Viral Production in Marine Sediments | p. 317 |
Chapter 34 Determination of Living/Dead and Active/Dormant Bacterial Fractions in Marine Sediments | p. 323 |
Chapter 35 Prokaryotic Biomass in Marine Sediments | p. 331 |
Chapter 36 Benthic Prokaryotic Heterotrophic Production Using the Leucine Incorporation Method | p. 337 |
Chapter 37 Prokaryotic Chemoautotrophic Production in Marine Sediments | p. 343 |
Chapter 38 Meiofaunal Biomass and Secondary Production | p. 351 |
Chapter 39 Macrofaunal and Megafaunal Biomass and Biochemical Composition | p. 369 |
Chapter 40 Protistan Grazing on Benthic Prokaryotes and Viruses | p. 377 |
Chapter 41 Meiobenthic Preoption on Prokaryotes | p. 385 |
Chapter 42 Analysis of Benthic Food Webs and Benthic Trophodynarmcs | p. 393 |
Section VI Suggested Readings for the Study of Deep-Sea Biodiversity and Ecosystem Functioning | |
Suggested Readings | p. 405 |
Index | p. 417 |