Cover image for Animal cell culture: a practical approach
Title:
Animal cell culture: a practical approach
Edition:
3rd.ed
Publication Information:
Oxford : Oxford University Press, 2000
ISBN:
9780199637973

9780199637966
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30000004880146 QH585.2 A55 2000 Open Access Book Book
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Summary

Summary

This new edition of Animal Cell Culture covers new or updated chapters on cell authentication, serum-free culture, apoptosis assays, FISH, genetic modification, scale-up, stem cell assays, 3-dimensional culture, tissue engineering and cytotoxicity assays. Detailed protocols for a wide variety of methods provide the core of each chapter, making new methodology easily accessible. Everyone working in biological and medical research, whether in academia or a commercial organization, practicing cell culture will benefit greatly from this book.


Table of Contents

List of protocols
Abbreviations
1 Introduction to basic principlesR. Ian Freshney
2 Scaling-up of animal cell culturesBryan Griffiths
3 Cell line preservation and authenticationR. J. Hay et al.
4 Development of serum-free mediaSoverin Karmiol
5 Three-dimensional cultureL. A. Kunz-Schughart and W. Mueller-Kleiser
6 Tissue engineeringRobert A. Brown and Rebecca A. Porter
7 Cytotoxicity and viability assaysAnne P. Wilson
8 Fluorescence in situ hybridizationW. Nicol Keith
9 Genetic modificationMajid Hafezparast
10 Epithelial stem cell identification, isolation, and cultureDavid Hudson
11 Senescence, apoptosis, and necrosisIan R. Kill and Richard G. A. Faragher
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Index