Title:
Bone research protocols
Series Title:
Methods in molecular medicine ; 80
Series:
Methods in molecular medicine ; 80
Publication Information:
Totowa, N.J. : Humana Press, c2003
Physical Description:
xiv, 448 p. : ill. ; 23 cm.
ISBN:
9781588290441
Available:*
Library | Item Barcode | Call Number | Material Type | Item Category 1 | Status |
---|---|---|---|---|---|
Searching... | 30000010235238 | QP88.2 B66 2003 | Open Access Book | Book | Searching... |
On Order
Summary
Summary
Laboratory techniques presented here will help researchers investigating the mechanisms by which certain genes affect bone mass and strength. These reproducible methods cover such topics as the isolation and culture of bone cells, the preparation of bone tissue for histological testing, and the use
Table of Contents
Preface | p. v |
Contributors | p. ix |
Part I Culture of Osteoblasts and Osteocytes | |
1 Human Osteoblast Culture | p. 3 |
2 Osteoblast Isolation from Murine Calvariae and Long Bones | p. 19 |
3 Mineralizing Fibroblast-Colony-Forming Assays | p. 29 |
4 Osteocyte Isolation and Culture | p. 41 |
Part II Culture of Osteoclasts | |
5 Isolated Osteoclast Cultures | p. 53 |
6 Primary Isolation and Culture of Chicken Osteoclasts | p. 65 |
7 Isolation and Purification of Rabbit Osteoclasts | p. 89 |
8 Generating Human Osteoclasts from Peripheral Blood | p. 101 |
9 Generating Human Osteoclasts from Bone Marrow and Peripheral Blood | p. 113 |
10 Generating Murine Osteoclasts from Bone Marrow | p. 129 |
11 Osteoclast Formation in the Mouse Coculture Assay | p. 145 |
12 RANKL-Mediated Osteoclast Formation from Murine RAW 264.7 Cells | p. 153 |
Part III Bone Organ Culture | |
13 Analysis of Osteoclast Function in Mouse Calvarial Cultures | p. 169 |
14 Assessing Bone Formation Using Mouse Calvarial Organ Culture | p. 183 |
Part IV Histological and Ultrastructural Techniques | |
15 In Situ Hybridization and In Situ Reverse Transcription Polymerase Chain Reaction in Human Bone Sections | p. 201 |
16 Techniques for the Study of Apoptosis in Bone | p. 225 |
17 Protein Localization in Wax-Embedded and Frozen Sections of Bone Using Immunohistochemistry | p. 237 |
18 Detection of Noncollagenous Bone Proteins in Methylmethacrylate-Embedded Human Bone Sections | p. 249 |
19 Fluorescence Imaging of Bone-Resorbing Osteoclasts by Confocal Microscopy | p. 259 |
20 Bone Histomorphometry | p. 283 |
21 Transmission Electron Microscopy of Bone | p. 299 |
22 Scanning Electron Microscopy of Bone | p. 311 |
Part V Imaging Techniques | |
23 Bone Measurements by Peripheral Quantitative Computed Tomography in Rodents | p. 323 |
Part VI In Vivo Techniques | |
24 Studies of Local Bone Remodeling: The Calvarial Injection Assay | p. 345 |
25 Inflammation-Induced Osteoporosis: The IMO Model | p. 353 |
26 Ovariectomy and Estrogen Replacement in Rodents | p. 361 |
Part VII Mechanical Loading Techniques | |
27 Mechanical Testing of Bone Ex Vivo | p. 369 |
28 Bone Cell Responses to Fluid Flow | p. 381 |
29 Methods for Analyzing Bone Cell Responses to Mechanical Loading Using In Vitro Monolayer and Organ Culture Models | p. 399 |
Part VIII Molecular Analysis | |
30 Extraction of Nucleic Acids from Bone | p. 425 |
31 Analysis of Gene Expression in Bone by Quantitative RT-PCR | p. 433 |
Index | p. 441 |