Available:*
Library | Item Barcode | Call Number | Material Type | Item Category 1 | Status |
---|---|---|---|---|---|
Searching... | 30000010274378 | QH440.5 G46 2001 | Open Access Book | Book | Searching... |
On Order
Summary
Summary
We must unashamedly admit that a large part of the motivation for editing Genomics Protocols was selfish. The possibility of assembling in a single volume a unique and comprehensive collection of complete protocols, relevant to our work and the work of our colleagues, was too good an opportunity to miss. We are pleased to report, however, that the outcome is something of use not only to those who are experienced practitioners in the genomics field, but is also valuable to the larger community of researchers who have recognized the potential of genomics research and may themselves be beginning to explore the technologies involved. Some of the techniques described in Genomics Protocols are clearly not restricted to the genomics field; indeed, a prerequisite for many procedures in this discipline is that they require an extremely high throughput, beyond the scope of the average investigator. However, what we have endeavored here to achieve is both to compile a collection of procedures concerned with geno- scale investigations and to incorporate the key components of "bottom-up" and "top-down" approaches to gene finding. The technologies described extend from those traditionally recognized as coming under the genomics umbrella, touch on proteomics (the study of the expressed protein complement of the genome), through to early therapeutic approaches utilizing the potential of genome programs via gene therapy (Chapters 27-30).
Table of Contents
Preface | p. v |
Contributors | p. xi |
1 Construction of Microsatellite-Based, High-Resolution Genetic Maps in the Mouse | p. 1 |
2 Genetic Analysis of Complex Traits | p. 11 |
3 Sequence-Based Detection of Single Nucleotide Polymorphisms | p. 29 |
4 Genomic Mismatch Scanning for the Mapping of Genetic Traits | p. 37 |
5 Detection of Chromosomal Abnormalities by Comparative Genomic Hybridization | p. 47 |
6 Construction of a Bacterial Artificial Chromosome Library | p. 57 |
7 Contiguation of Bacterial Clones | p. 69 |
8 Mapping of Genomic Clones by Fluorescence In Situ Hybridization | p. 109 |
9 Map Integration: From a Genetic Map to a Physical Gene Map and Ultimately to the Sequence Map | p. 129 |
10 Construction of Full-Length-Enriched cDNA Libraries: The Oligo-Capping Method | p. 143 |
11 Construction of Transcript Maps by Somatic Cell/Radiation Hybrid Mapping: The Human Gene Map | p. 155 |
12 Preparation and Screening of High-Density cDNA Arrays with Genomic Clones | p. 169 |
13 Direct Selection of cDNAs by Genomic Clones | p. 189 |
14 Exon Trapping: Application of a Large-Insert Multiple-Exon-Trapping System | p. 201 |
15 Sequencing Bacterial Artificial Chromosomes | p. 217 |
16 Finding Genes in Genomic Nucleotide Sequences by Using Bioinformatics | p. 235 |
17 Gene Identification Using the Pufferfish, Fugu rubripes, by Sequence Scanning | p. 249 |
18 Isolation of Differentially Expressed Genes Through Subtractive Suppression Hybridization | p. 263 |
19 Isolation of Differentially Expressed Genes by Representational Difference Analysis | p. 279 |
20 Expression Profiling and the Isolation of Differentially Expressed Genes by Indexing-Based Differential Display | p. 295 |
21 Expression Profiling by Systematic High-Throughput In Situ Hybridization to Whole-Mount Embryos | p. 309 |
22 Expression Monitoring Using cDNA Microarrays: A General Protocol | p. 323 |
23 Prediction of Protein Structure and Function by Using Bioinformatics | p. 341 |
24 Identification of Novel Genes by Gene Trap Mutagenesis | p. 377 |
25 Determination of Gene Function by Homologous Recombination Using Embryonic Stem Cells and Knockout Mice | p. 397 |
26 Genomic Analysis Utilizing the Yeast Two-Hybrid System | p. 415 |
27 Methods for Adeno-Associated Virus-Mediated Gene Transfer into Muscle | p. 455 |
28 Retroviral-Mediated Gene Transduction | p. 471 |
29 Gene Therapy Approaches to Sensitization of Human Prostate Carcinoma to Cisplatin by Adenoviral Expression of p53 and by Antisense Jun Kinase Oligonucleotide Methods | p. 495 |
30 Ribozyme Gene Therapy | p. 521 |
Index | p. 531 |