Lluis M. MirMarie-Pierre RolsDietmar RabussayLeda Raptis and Kevin L. FirthKhursheed AnwerSek-Wen HuiRaji SundararajanHenrike Siemen and Lars Nolden and Stefanie Terstegge and Philipp Koch and Oliver BrustleLinda N. Liu and Rama Shivakumar and Cornell Allen and Joseph C. FratantoniAnnelies Michiels and Sandra Tuyaerts and Aude Bonehill and Carlo Heirman and Jurgen Corthals and Kris ThielemansKathrin Schoenberg and Hans-Ingo Trompeter and Markus UhrbergLeda Raptis and Adina Vultur and Heather L. Brownell and Evangelia Tomai and Aikaterini Anagnostopoulou and Rozanne Arulanandam and Jun Cao and Kevin L. FirthJames G. GrannemanCapucine Trollet and Daniel Scherman and Pascal BigeyPatricia A. Brown and Amir S. Khan and Ruxandra Draghia-AkliBabu M. Medi and Jagdish SinghRui Zhou and James E. Norton and David A. DeanMasaki Yoshida and Hitoshi Iwashita and Masayuki Otani and Koichi Masunaga and Akito InadomeTakahiko Matsuda and Constance L. CepkoMuriel Golzio and Justin TeissieN. Madhusudhana Rao and K. Murali Rambabu and S. Harinarayana RaoAbie Craiu and David ScaddenShulin LiJeffry Cutrera and Marina Torrero and Keijiro Shiomitsu and Neal Mauldin and Shulin LiShiguo Zhu and Shulin LiMarina Torrero and Shulin LiJulie GehlHolly M. Horton and Peggy A. Lalor and Alain P. RollandJason G. FewellGuy P. Marti and Parsa Mohebi and Lixin Liu and Jiaai Wang and Tomoharu Miyashita and John W. HarmonKazuo Komamura and Jun-ichi Miyazaki and Enyu Imai and Kunio Matsumoto and Toshikazu and Nakamura and Masatsugu HoriRobert M.I. Kapsa and Sharon H.A. Wong and Anita F. QuigleyKim E. Wells and Jill McMahon and Helen Foster and Aurora Ferrer and Dominic J. WellsVadim V. Fedorov and Vladimir P. Nikolski and Igor R. EfimovRuxandra Draghia-Akli and Amir S. KhanAnna-Karin Roos and Alan King and Pavel PisaArianna Smorlesi and Francesca Papalini and Sara Pierpaoli and Mauro ProvincialiYong-Gang Zhao and Yuhong XuTorunn Elisabeth Tjelle and Dietmar Rabussay and Christian Ottensmeier and Iacob Mathiesen and Rune KjekenKaw Yan Chua and John D.A. Ramos and Nge Cheong

Preface	p. v
Contributors	p. xiii
Part I Basic Concepts of DNA Transfer via Electroporation
Chapter 1 Application of Electroporation Gene Therapy: Past, Current, and Future	p. 3
Chapter 2 Mechanism by Which Electroporation Mediates DNA Migration and Entry into Cells and Targeted Tissues	p. 19
Chapter 3 Applicator and Electrode Design for In Vivo DNA Delivery by Electroporation	p. 35
Chapter 4 Electrode Assemblies Used for Electroporation of Cultured Cells	p. 61
Chapter 5 Formulations for DNA Delivery via Electroporation In Vivo	p. 77
Chapter 6 Overview of Drug Delivery and Alternative Methods to Electroporation	p. 91
Chapter 7 Nanoelectroporation: A First Look	p. 109
Part II In Vitro Targeted Gene Delivery via Electroporation
Chapter 8 Nucleofection of Human Embryonic Stem Cells	p. 131
Chapter 9 Delivery of Whole Tumor Lysate into Dendritic Cells for Cancer Vaccination	p. 139
Chapter 10 Delivery of Tumor-Antigen-Encoding mRNA into Dendritic Cells for Vaccination	p. 155
Chapter 11 Delivery of DNA into Natural Killer Cells for Immunotherapy	p. 165
Chapter 12 Electroporation of Adherent Cells In Situ for the Study of Signal Transduction and Gap Junctional Communication	p. 173
Chapter 13 Delivery of DNA into Adipocytes within Adipose Tissue	p. 191
Part III In Vivo Targeted Gene Delivery via Electroporation
Chapter 14 Delivery of DNA into Muscle for Treating Systemic Diseases: Advantages and Challenges	p. 199
Chapter 15 Delivery of DNA into Skeletal Muscle in Large Animals	p. 215
Chapter 16 Delivery of DNA into Skin via Electroporation	p. 225
Chapter 17 Electroporation-Mediated Gene Delivery to the Lungs	p. 233
Chapter 18 Delivery of DNA into Bladder via Electroporation	p. 249
Chapter 19 Analysis of Gene Function in the Retina	p. 259
Chapter 20 Optical In Vivo Imaging of Electrically Mediated Delivery of siRNA into Muscle for Gene Function Analysis	p. 279
Chapter 21 Electroporation of Adult Zebrafish	p. 289
Part IV Treatment of Cancer via Electroporation Gene Therapy
Chapter 22 Flow Electroporation with Pulsed Electric Fields for Purging Tumor Cells	p. 301
Chapter 23 Delivery of DNA into Tumors	p. 311
Chapter 24 Intratumoral Bleomycin and IL-12 Electrochemogenetherapy for Treating Head and Neck Tumors in Dogs	p. 319
Chapter 25 Systemic IL-12 Gene Therapy for Treating Malignancy via Intramuscular Electroporation	p. 327
Chapter 26 Treatment of SCCVII Tumors with Systemic Chemotherapy and Interleukin-12 Gene Therapy Combination	p. 339
Chapter 27 Electroporation for Drug and Gene Delivery in the Clinic: Doctors Go Electric	p. 351
Chapter 28 IL-2 Plasmid Electroporation: From Preclinical Studies to Phase I Clinical Trial	p. 361
Part V Treatment of Other Diseases via Electroporation Gene Therapy
Chapter 29 Factor IX Gene Therapy for Hemophilia	p. 375
Chapter 30 KGF-1 for Wound Healing in Animal Models	p. 383
Chapter 31 Hepatocyte Growth Factor Gene Therapy for Hypertension	p. 393
Chapter 32 Electroporation of Corrective Nucleic Acids (CNA) In Vivo to Promote Gene Correction in Dystrophic Muscle	p. 405
Chapter 33 Gene Delivery to Dystrophic Muscle	p. 421
Chapter 34 Effect of Electroporation on Cardiac Electrophysiology	p. 433
Chapter 35 Muscle and Fat Mass Modulation in Different Clinical Models	p. 449
Part VI Applications of Electroporation for DNA Vaccination
Chapter 36 DNA Vaccination for Prostate Cancer	p. 463
Chapter 37 HER2/neu DNA Vaccination for Breast Tumors	p. 473
Chapter 38 Electroporation-Mediated HBV DNA Vaccination in Primate Models	p. 487
Chapter 39 Taking Electroporation-Based Delivery of DNA Vaccination into Humans: A Generic Clinical Protocol	p. 497
Chapter 40 Production of Monoclonal Antibody by DNA Immunization with Electroporation	p. 509
Index	p. 521