Guide to yeast genetics and molecular biology

This guide presents a compilation of the protocols and procedures that have made saccharomyce cerevisiae such a facile system for all researchers in yeast molecular and cell biology. It is designed to meet the needs of the newcomer, experienced researcher, and graduate student. Alternative procedures in genetics, cloning, cell biology, microscopy, and organelle separation (ie nuclei) are compared and their relative merits and disadvantages clearly described.

Basic Methods of Yeast GeneticsF. Sherman

Getting Started with YeastF. Sherman and J. Hicks

Micromanipulation and Dissection of AsciF. Sherman and P. Wakem

Mapping Yeast GenesS.L. Gerring and C. Connelly and P. Hieter

Positional Mapping of Genes by Chromosome Blotting and Chromosome FragmentationG.F. Sprague

Assay of Yeast Mating ReactionY. Kassir and G. Simchen

Monitoring Meiosis and Sporulation in Saccharomyces cerevisiaeR.E. Esposito and M. Dresser and M. Breitenbach

Identifying Sporulation Genes

Visualizing Synaptonemal Complexes

and Large-Scale Spore and Spore Wall PurificationI. Herskowitz and R.E. Jensen

Putting the HO Gene to Work: Practical Uses for Mating-Type SwitchingB. Rockmill and E.J. Lambie and G.S. Roeder

Spore EnrichmentT.D. Fox and L.S. Folley and J.J. Mulero and T.W. McMullin and P.E. Thorsness and L.O. Hedin and M.C. Costanzo

Analysis and Manipulation of Yeast Mitochondrial Genes

Cloning and Recombinant DNAP. Philippsen and A. Stotz and C. Scherf

DNA of Saccharomyces cerevisiaeD.M. Becker and L. Guarente

High-Efficiency Transformation of Yeast by ElectroporationJ.A. Heinemann and G.F. Sprague

Transmission of Plasmid DNA to Yeast by Conjugation with BacteriaM.D. Rose and J.R. Broach

Cloning Genes by Complementation in YeastR.A. Young and R.W. Davis

Gene Isolation with

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Summary

Summary

Table of Contents