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Library | Item Barcode | Call Number | Material Type | Item Category 1 | Status |
|---|---|---|---|---|---|
Searching... | 30000010297960 | QP601 P35 2007 | Open Access Book | Book | Searching... |
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Summary
Summary
In recent years, there have been considerable developments in techniques for the investigation and utilisation of enzymes. With the assistance of a co-author, this popular student textbook has been updated to include techniques such as membrane chromatography, aqueous phase partitioning, engineering recombinant proteins for purification and due to the rapid advances in bioinformatics/proteomics, a discussion of the analysis of complex protein mixtures by 2D-electrophoresis and RPHPLC prior to sequencing by mass spectroscopy. Written with the student firmly in mind, no previous knowledge of biochemistry, and little of chemistry, is assumed. It is intended to provide an introduction to enzymology, and a balanced account of all the various theoretical and applied aspects of the subject which are likely to be included in a course.
Reviews 1
Choice Review
Enzymology is taught in most biochemistry graduate programs, and a new comprehensive work in this area would be welcome. In this second edition, (1st ed., 2001), Palmer and Bonner (both, Nottingham Trent Univ.) adequately cover what most biochemists might term the fundamentals of enzyme kinetics. The authors do a good job describing the history of the field, analyzing simple enzyme substrate interaction, and including some coverage of enzyme regulation. The book fails to provide information for someone working in the field today, where the systems are infinitely more complex and require new, sophisticated methods of analysis. Many of the enzymes studied today act on other proteins or enzymes, and many form large heterogeneous protein complexes. The chapters on protein structure determination are not comprehensive enough for today's students, and they do not show the correct ionization state for the amino acids they reference. The content of the book is somewhat odd in that some of the later chapters, which discuss enzyme isolation and analysis, are better suited for a laboratory manual rather than a course resource. In general, this book covers well what was, but poorly what is. It is marginally suitable for a lower-level enzymes/enzyme kinetics course. Summing Up: Optional. Lower-division undergraduates; technical program students. J. M. Tomich Kansas State University
