DNA science : a first course

The increased military employment of remotely operated aerial vehicles, also known as drones, has raised a wide variety of important ethical questions, concerns, and challenges. Many of these have not yet received the serious scholarly examination such worries rightly demand. This volume

attempts to fill that gap through sustained analysis of a wide range of specific moral issues that arise from this new form of killing by remote control. Many, for example, are troubled by the impact thatkilling through the mediated mechanisms of a drone half a world away has on the pilots who fly

them. What happens to concepts such as bravery and courage when a war-fighter controlling a drone is never exposed to any physical danger?

This dramatic shift in risk also creates conditions of extreme asymmetry between those who wage war and those they fight. What are the moral implications of such asymmetry on the military that employs such drones and the broader questions for war and a hope for peace in the world going forward? How

does this technology impact the likely successes of counter-insurgency operations or humanitarian interventions? Does not such weaponry run the risk of making war too easy to wage and tempt policy makers into killing when other more difficult means should be undertaken?

Killing By Remote Control directly engages all of these issues. Some essays discuss the just war tradition and explore whether the rise of drones necessitates a shift in the ways we think about the ethics of war in the broadest sense. Others scrutinize more specific uses of drones, such as their

present use in what are known as "targeted killing" by the United States. The book similarly tackles the looming prospect of autonomous drones and the many serious moral misgivings such a future portends.

Author Notes

David A. Crotty: Cold Spring Harbor Laboratory
David Micklos is Executive Director of the Dolan DNA Learning Center at Cold Spring Harbor Laboratory
Greg Freyer is Associate Professor of Clinical Environmental Health Sciences and Anatomy and Cell Biology at Columbia University College of Physicians & Surgeons

Dedication	p. v
Preface	p. ix
Acknowledgments	p. xi
Chapter 1 How We Learned That DNA Is the Genetic Material	p. 3
Chapter 2 How We Learned the Function of DNA	p. 37
Chapter 3 How We Learned How Genes Are Regulated	p. 69
Chapter 4 Basic Tools and Techniques of DNA Science	p. 107
Chapter 5 Methods for Finding and Expressing Important Genes	p. 141
Chapter 6 Modern Methods for Analyzing Whole Genomes	p. 183
Chapter 7 The DNA Science of Cancer	p. 221
Chapter 8 Applying DNA Science to Human Genetics and Evolution	p. 259
Laboratories: Lab Safety and Adherence to National Institutes of Health Guidelines	p. 315
1 Measurements, Micropipetting, and Sterile Techniques	p. 321
2 Bacterial Culture Techniques	p. 331
A. Isolation of Individuals Colonies	p. 333
B. Overnight Suspension Culture	p. 340
C. Mid-log Suspension Culture	p. 345
3 DNA Restriction Analysis	p. 351
4 Effects of DNA Methylation on Restriction	p. 375
5 Rapid Colony Transformation of E. coli with Plasmid DNA	p. 385
6 Assay for an Antibiotic Resistance Enzyme	p. 399
7 Purification and Identification of Recombinant GFP	p. 411
A. Purification of GFP by HIC	p. 412
B. PAGE Analysis of Purified GFP	p. 417
8 Purification and Identification of Plasmid DNA	p. 423
A. Plasmid Minipreparation of pAMP	p. 424
B. Restriction Analysis of Purified pAMP	p. 431
9 Recombination of Antibiotic Resistance Genes	p. 443
A. Restriction Digest of Plasmids pAMP and pKAN	p. 445
B. Ligation of pAMP and pKAN Restriction Fragments	p. 452
10 Transformation of E. coli with Recombinant DNA	p. 457
A. Classic Procedure for Preparing Competent Cells	p. 458
B. Transformation of E. coli with Recombinant DNA	p. 463
11 Replica Plating to Identify Mixed E. coli Populations	p. 473
12 Purification and Identification of Recombinant DNA	p. 481
A. Plasmid Minipreparation of pAMP/pKAN Recombinants	p. 482
B. Restriction Analysis of Purified Recombinant DNA	p. 487
Answers to Discussion Questions	p. 501
Appendices
1 Equipment, Supplies, and Reagents	p. 521
2 Recipes for Media, Reagents, and Stock Solutions	p. 529
3 Restriction Map Data for pAMP, pKAN, pBLU, pGREEN, and Bacteriophage [lambda]	p. 547
4 Cautions	p. 563
Name Index	p. 559
Subject Index	p. 561

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Author Notes

Table of Contents