Borisova VV and Frank LA and Markova SV and Golz S and Vysotski ESEremeeva EV and Markova SV and Frank LA and Vysotski ESHirano T and Takahashi Y and Kondo H and Ikeda H and Maki S and Niwa HLomakina GY and Legotsky SA and Tishkov VI and Ugarova NNOba Y and Inouye SSagegami-Oba R and Oba Y and Ohira HSukovataya IE and Tyulkova NA and Kaykova EVSukovataya IE and Tyulkova NA and Kaykova EVWhitaker S and Herring PJ and Campbell AK and Hill PJ and Rees CEDBerthold F and Hafner K and Schreibers M and Tarkkanen VHatta-Ohashi Y and Hayasaka N and Takahasi T and Shigeyoshi Y and Suzuki HHickey PCSuzuki H and Dosaka S and Ohashi-Hatta Y and Sugiyama TAdamczyk M and Brashear RJ and Mattingly PGAdamczyk M and Brashear RJ and Mattingly PG and Tsatsos PHArakawa H and Munakata E and Obinata R and Maeda M and Suzuki S and Kamahori M and Kambara HBrovko LY and Hajdok C and Tapp C and Ramsay C and Driscoll M and Griffiths MWBrovko LY and Romanova NA and Allain B and Mandeville R and Griffiths MWDriscoll M and Ramsay C and Watkin JEckert CR and Esteves CH and Bastos EL and Romoff P and Baader WJFoote N and Green M and Gregory SFrundzhyan VG and Ugarova NN and Makarushin AA and Gavrilov GBGandelman O and Church VL and Moore CA and Carne C and Jalal H and Murray JAH and Tisi LCHawkins EM and Garvin D and Paguio A and Stecha P and Swanson B and Fan F and Wood KVIto K and Nishimura W and Maeda M and Gomi K and Inouye S and Arakawa HJathoul AP and Gandelman OA and Law GHE and Murray JAH and Tisi LCKumari A and Pasini P and Deo SK and Flomenhoft D and Shashidhar H and Daunert SMastroianni N and Flak T and Corazza SMichelini E and Southworth TL and Ablamski D and Branchini BR and Roda AMichelini E and Magliulo M and Baraldini M and Roda ARobinson GM and Jackson SK and Salisbury VC and Nelson SMRowe L and Rothert A and Deo SK and Ensor M and Daunert SSatienperakul S and Liawruangrath S and Cardwell TJShah D and Chang C-D and Cheng K and Jiang L and Salbilla V and Haller A and Schochetman GSun Y and Jacobson KB and Golovlev VSun X and Yang XTanaka M and Igarashi R and Wada NTeranishi KTricoire L and Tsuzuki K and Lambolez BVetrova EV and Prokhorova EVWu C and Kawasaki K and Ohgiya S and Ohmiya YZomer G and Smitsman C and Arts R and Hamzink M and Kooijman MAsai T and Berthold A and Ohmiya YChen N and Yu YA and Zhang Q and Raab V and Szalay AACutter KL and Nelson SM and Greenman J and Robinson GM and Salisbury VCDrobac E and Tricoire L and Lambolez BGaddipati SR and Perehinec TM and Qazi SNA and Rees CED and Hill PJRaab V and Horbaschek C and Chen N and Zhang Q and Yu YA and Seubert C and Geissinger U and Worschech A and Tietze CJ and Zellner E and Le TH|cGrummt F and Stritzker J and Szalay AARoda A and Guardigli M and Mirasoli M and Ambretti S and Venturoli S and Musiani M and Casanova S and Roda EStritzker J and Weibel S and Hill PJ and Szalay AAYu YA and Szalay AABastos EL and Ciscato LFML and Bartoloni FH and Baader WJCiscato LFML and Bartoloni FH and Baader WJCiscato LFML and Weiss D and Flammersheim HJ and Baader WJNozaki O and Munesue M and Kawamoto HOliveira MA and Baader WJRahimi Y and Shrestha S and Deo SKShibata R and Yoshida Y and Wada NWada N and Fujii H and Sakai HWeiss D and Trupp S and Ciscato LFML and Beckert R and Anders EYoshiki YKratasyuk VA and Gusev SM and Remmel NN and Osipenko OA and Esimbekova EN and Shoeman DM and Dreschel TW and Chetirkin PV and Correll MJ and Bucklin RA and Rygalov VEPiontkovski SA and Tokarev YN and Levin LAStevani CV and de Oliveira AG and Mendes LF and Domingues OTorres E and Gonzalez VL

Preface	p. v
Introduction to the 14th Symposium	p. ix
Part 1 Luciferases
Refolding of the recombinant luciferases of Metridia longa	p. 3
The main function of HIS175, TRP179, and TYR190 residues of the obelin binding site is to stabilize the hydroperoxycoelenterazine intermediate	p. 7
Mechanistic study on the Cypridina (Vargula) bioluminescence reaction	p. 11
Stabilization of Luciola mingrelica firefly luciferase by mutation of non-conservative cysteine residues	p. 15
Firefly luciferase arose from fatty acyl-CoA synthetase by gene duplication and gene recruitment	p. 19
Bioluminescence in click beetles (family Elateridae): Molecular phylogenetic analysis, inferred from 28S ribosomal DNA	p. 23
Effect of microenvironmental changes on kinetic parameters of steady-state enzyme-induced bacterial bioluminescent reaction	p. 27
Thermal stability and absorption spectra of bacterial luciferases from Photobacterium leiognathi in water-organic solvents	p. 31
Characterization of a fish symbiont of Photobacterium phosphoreum with altered spectral properties	p. 35
Part 2 Instrumentation
Luminometers for industrial applications of rapid microbiology	p. 41
Application of a luminescence microscope with novel optical system for detection of the gene expression pattern of individual cells	p. 45
Novel tools for quantification of luminescence	p. 49
Luminescence microscope for reporter assay of single live cells	p. 53
Part 3 Assays
Elevation of choline concentration in cardiac troponin-I positive human plasma	p. 59
High throughput detection of hydrogen peroxide: Validation of homogeneous chemiluminescent assays for choline in human plasma and whole blood	p. 63
Development of bioluminescent pyrophosphate assay using pyruvate phosphate dikinase and its application to SNPs analysis	p. 67
Bioluminescent method for detection of proteinase activity in UHT milk	p. 71
Detection and control of Salmonella typhimurium growth using specific bacteriophage	p. 75
Improved sensitivity method for rapid hygiene monitoring using ATP bioluminescence	p. 79
Determination of antioxidant capacity and reactivity using hemincatalyzed luminol chemiluminescence	p. 83
Applications of adenylate kinase detection using bioluminescence	p. 87
Standardization of bioluminescent raw milk quality assay using Lumtek test-systems	p. 91
BART - Bioluminescent alternative to real-time PCR	p. 95
Advancing the development of dual-luciferase assays	p. 99
Simultaneous bioluminescent immunoassay using aequorin labeled Fab fragment and biotinylated firefly luciferase	p. 103
Designing enhanced thermostable luciferase for proteolytic assay	p. 107
Biosensors for the non-invasive evaluation of bacterial quorum sensing in GI disorders	p. 111
Improved Ca[superscript 2+]-activated photoproteins for HTS applications	p. 115
Development of a multiplexed bioluminescent cell-based assay with the luc gene from Luciola italica for high throughput screening of cholesterol-lowering drugs	p. 119
Novel BRET-based biosensors with a new bioluminescent donor, Gaussia luciferase, for estrogen receptor ligands	p. 123
The use of lux genes for monitoring uptake of Pseudomonas aeruginosa by respiratory epithelial cells	p. 127
Bioluminescence characteristics of an Obelin mutant in varying solvent conditions	p. 131
A simple chemiluminescence flow injection analysis for the determination of sulphide in aqueous samples	p. 135
Using recombinant antigens for the detection of antibodies to Trypanosomas cruzi on a fully automated chemiluminescence analyzer	p. 139
Quantitative bioluminescent RNA assay	p. 143
A novel multi-channel chemiluminescence-immunoassay to detect HIV antibodies as a more specific supplemental test	p. 147
Time-resolved measurement of firefly bioluminescence using photolysis of caged-ATP	p. 151
Red-chemiluminescent probes for detecting superoxide anions	p. 155
Aequorin bioluminescence dissected by random mutagenesis	p. 159
Influence of inorganic sulfur compounds on bioluminescent system NAD(P)H: FMN-oxidoreductase-luciferase	p. 163
Development of the Cypridina bioluminescent system for high throughput screening assays	p. 167
Quantitative Western blotting using a GZ-11 based chemiluminogenic signal reagent	p. 171
Part 4 Optical Imaging and Multicolor Assays
Demonstration of tri-colored reporter assay using a filter luminometer "Sirius C"	p. 177
Real-time monitoring of vaccinia virus infection in cultured cells and in living mice using light-emitting proteins	p. 181
Use of bioluminescent reporter bacteria to study invasion and survival within mammalian cell lines	p. 185
Network activities of the cerebral cortex monitored by bioluminescent aequorin imaging	p. 189
Bivalent fluorescent reporters for gene expression studies in Gram-positive bacteria	p. 193
Four-color labeling of cell culture and tumors of live mice upon infection with: GFP-Ruc and RFP-CBG99 expressing Vaccinia virus strains	p. 197
Quantitative immunohistochemistry and in situ hybridization with luminescence detection: a new potential diagnostic tool	p. 201
Evaluation of an in vivo gene induction system in infected tumor-bearing mice	p. 205
Examinations of bacterium-mediated detection of tumors in mice models	p. 209
Part 5 Biochemistry/Luminescence
Direct observation of a radical-ion intermediate in the chemiexcitation step of peroxyoxalate chemiluminescence	p. 215
Kinetic observation of the chemiexcitation step in peroxyoxalate chemiluminescence	p. 219
Synthesis and studies of fenchyl-derived 1,2-dioxetanes	p. 223
Enzyme embedded organic monolith sensor for flow injection -chemiluminescence	p. 227
Efficiency of electron-transfer induced chemiexcitation: A comparison of inter- and intramolecular processes	p. 231
Study of metal binding to monomeric red fluorescent protein, DsRed-monomer	p. 235
Time-dependent spectral change of chemiluminescence from firefly luciferin in deoxygenated dimethyl sulfoxide	p. 239
A quantum-chemical approach to the amino analogs of firefly luciferin	p. 243
Firefly-luciferin and its analogs: a source of new luminescence dyes and ligands	p. 247
Chemiluminescence enhancement effect of trace metal elements	p. 251
Part 6 BLCL Oceans, Land & Space
Bioluminescence in the spaceflight and life science training program at the Kennedy Space Center	p. 257
A comparison of bioluminescence and chlorophyll fields of the world ocean	p. 261
Brazilian species of bioluminescence fungi	p. 265
Molecular phylogeny of cypridinid ostracodes and the evolution of bioluminescence	p. 269
Index	p. 273