Available:*
Library | Item Barcode | Call Number | Material Type | Item Category 1 | Status |
|---|---|---|---|---|---|
Searching... | 30000003367483 | QH585.5.F56 F56 1995 | Open Access Book | Book | Searching... |
Searching... | 30000003367442 | QH585.5.F56 F56 1995 | Open Access Book | Book | Searching... |
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Summary
Summary
Flow Cytometry, Second Edition provides a complete and comprehensive two volume laboratory guide and reference for the use of the most current methods in flow cytometry sample preparation and analysis. These essential techniques are described in a step-by-step format, supplemented by explanatory sections and trouble-shooting tips. The methods are accessible to all researchers and students in biomedical science and biology who must use flow cytometry to separate and analyze cells.
Key Features
* Completely revised and greatly expanded since the publication of the First Edition in 1990
* Methods cover cell death and cell cycle analyses
Practical, handbook-style presentation works in lab or classroom
* Unique comprehensive methodological coverage
* Color plates illustrate techniques
* In-depth treatment of procedures, including a description of each procedure:
* Theoretical foundations
* Critical aspects
* Possible pitfalls
* Written by authors with extensive experience who:
* Developed or modified the techniques
* Describe their experience with different instruments and applications to different cell systems
* Are the "Who's Who" in Flow Cytometry
Table of Contents
| Dissociation of Intact Cells from Tumors and Normal TissuesD.W. Visscher and J.D. Crissman |
| Assays of Cell Viability: Discrimination of Cells Dying by ApoptosisZ. Darzynkiewicz and X. Li and J. Gong |
| Cell Preparation for the Identification of LeukocytesC.C. Stewart and S.J Stewart |
| Multiparameter Analysis of Leukocytes by Flow CytometryC.C. Stewart and S.J. Stewart |
| Immunophenotyping Using Fixed CellsG.F. Babcock and S.M. Dawes |
| Simultaneous DNA Content and Cell-Surface Immunofluorescence AnalysisP.P.T.Brons and P.E.J. Van Erp and A.H.M. Pennings |
| Flow Cytometry Crossmatching for Solid Organ TransplantationR.A. Bray |
| Cell Membrane Potential AnalysisH.M. Shapiro |
| Measurement of Intracellular pHM.J. Boyer and D.W. Hedley |
| Intracellular Ionized CalciumC.H. June and P.S. Rabinovitch |
| Cellular Protein Content MeasurementsH.A. Crissman and J.A. Steinkamp |
| Lysosomal Proton Pump Activity: Supravital Cell Staining with Acridine Orange Differentiates Leukocyte SubpopulationsF. Traganos and Z. Darzynkiewicz |
| Staining of DNA in Live and Fixed CellsH.A. Crissman and G.T. Hirons |
| High-Resolution Analysis of Nuclear DNA Employing the Fluorochrome DAPIF.J. Otto |
| Detergent and Proteolytic Enzyme-Based Techniques for Nuclear Isolation and DNA Content AnalysisL.L. Vindelov and I.J. Christensen |
| DNA Analysis from Paraffin-Embedded BlocksD.W. Hedley |
| Controls, Standards, and Histogram Interpretation in DNA Flow CytometryL.G. Dressler and L.C. Seamer |
| DNA Content Histogram and Cell-Cycle AnalysisP.S. Rabinovitch |
| Immunochemical Quantitation of Bromodeoxyuridine: Application to Cell-Cycle KineticsF. Dolbeare and J.R. Selden |
| Application and Detection of IdUrd and CldUrd as Two Independent Cell-Cycle MarkersJ.A. Aten and J. Stap and R. Hoebe and P.J.M. Bakker |
| Cell-CycleAnalysis Using Continuous Bromodeoxyuridine Labeling and Hoechst 33358(Ethidium Bromide Bivariate Flow CytometryM. Poot and H. Hoehn and M. Kubbies and A. Grossmann and Y. Chen and P.S. Rabinovitch and H. Kitano |
| Detection of BrdUrd-Labeled Cells by Differential Fluorescence Analysis of DNA Fluorochromes: Pulse-Chase and Continuous Labeling MethodsH.A. Crissman and N. Oishi and R. Habbersett |
| Analysis of Intracellular ProteinsK.D. Bauer and J.W. Jacobberger |
| Washless Procedures for Nuclear Antigen DetectionJ.K. Larsen |
| Light Scatter of Isolated Cell Nuclei as a Parameter Discriminating the Cell-Cycle SubcompartmentsW. Giaretti and M. Nusse |
| Simultaneous Analysis of Cellular RNA and DNA ContentZ. Darzynkiewicz |
| Analysis of DNA Content and Cyclin Protein Expression in Studies of DNA Ploidy, Growth Fraction, Lymphocyte Stimulation, and the Cell-CycleZ. Darzynkiewicz and J. Gong and F. Traganos |
| Oxidative Product Formation Analysis by Flow CytometryJ.P. Robinson and W.O. Carter and P.K. Narayanan |
| Flow Cytometric Determination of Cysteine and Serine Proteinase Activities in Living Cells with Rhodamine 110 SubstratesS. Klingel and G. Rothe and W. Kellermann and G. Valet |
| Leucine Aminopeptidase Activity by Flow CytometryJ.J. Turek and J.P. Robinson |
| On-Line Flow Cytometry: A Versatile Method for Kinetic MeasurementsJ.V. Watson and C. Dive and Enzyme Kinetics.K. Nooter and H. Herweijer and R.R. Jonker and G.J. van den Engh |
| Acid-Induced Denaturation of DNA in Situ as a Probe of Chromatin StructureZ. Darzynkiewicz |
| Detection of Intracellular Virus and Viral ProductsJ. Laffin and J.M. Lehman |
| Subject Index |
