Cover image for Clinical and forensic applications of capillary electrophoresis
Title:
Clinical and forensic applications of capillary electrophoresis
Series:
Pathology and laboratory medicine
Publication Information:
Totowa, NJ : Humana Press, 2001
Physical Description:
x, 453 p. : ill. ; 24 cm.
ISBN:
9780896036451

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30000010179601 RB43.8.C36 C54 2001 Open Access Book Book
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Summary

Summary

John R. Petersen and Amin A. Mohammad, along with a panel of leading basic and clinical investigators, review those CE methods that are now replacing many routine serum and blood tests in clinical and forensic laboratories. Major areas reviewed include the coating of columns; the analysis of serum, urine, and CSF proteins and paraproteins; abnormal hemoglobins and hemoglobin Alc; peptides, amino and organic acids; therapeutic drugs; drugs of abuse; viral load; and short tandem repeats (STR). The methods discussed include capillary zone, micellar, electrokinetic, capillary gel, and non-aqueous electrophoresis. Innovative and highly practical, Clinical and Forensic Applications of Capillary Electrophoresis demonstrates the power and versatility of CE-not only to develop new assays, but also to markedly simplify today's clinical and forensic laboratory methodology.


Table of Contents

Part I
Section I Introduction
Capillary Electrophoresis: General Overview and Applications in the Clinical LaboratoryManjiri Lele and Subodh M. Lele and John R. Petersen and Amin A. Mohammad
Basic Principles and Modes of Capillary ElectrophoresisHarry Whatley
Section II Protein Electrophoresis
Capillary Coatings for Protein AnalysisKannan Srinivasan and Chris Pohl and Nebojsa Avdalovic
Clinical Serum Protein Capillary Zone ElectrophoresisCarl R. Jolliff
Serum and Urine Paraprotein Capillary ElectrophoresisCarl R. Jolliff
Cerebrospinal Fluid Protein ElectrophoresisJohn R. Petersen and Amin A. Mohammad
Lipoprotein AnalysisRainer Lehmann
Clinical Analysis of Structural Hemoglobin Variants and Hb A1c by Capillary
Isoelectric FocusingJames M. Hempe and Alfonso Vargas and Randall D. Craver
Section III Metabolic Diseases
Amino Acid AnalysisMary Kathryn Linde
Organic AcidsKern L. Nuttall and Norberto A. Guzman
Steroids, Cecilla YouhAmin A. Mohammad and John R. Petersen
Section IV Immunoassay
Capillary Electrophoresis Based ImmunoassayBode Adesoji and Amin A. Mohammad and John R. Petersen
Section V Molecular Diagnostics
Quantitation of Viral LoadJill M. Kolesar
The Application of Capillary Electrophoresis in the Analysis of PCR Products used in Forensic DNA TypingBruce R. McCord and John M. Butler
Capillary Electrophoresis with Electrospray Ionization Mass SpectrometrySamir Cherkaoui
Section VI Mass Spectrometry, Therapeutic Drug Monitoring, and Toxicology
Capillary Electrophoresis-Mass Spectrometry of Biologically Active Peptides and ProteinsStephen Naylor and Andy J. Tomlinson
Serum Drug Monitoring by Capillary ElectrophoresisZack K. Shihabi
Applications of Capillary Zone Electrophoresis in the Analysis of Metal Ions of Clinical SignificanceLokinendi V. Rao and John R. Petersen and Amin A. Mohammad and Anthony O. Okorodudu
Clinical and Forensic Drug Toxicology: Analysis of Illicit and Abused Drugs in Urine by Capillary ElectrophoresisWolfgang Thormann and Jitka Caslavska
Screening Biological Specimens for Drugs of Forensic SignificanceJohn C. Hudson and Murray J. Malcolm and Mauro Golin
Index