Forensic and clinical applications of solid phase extraction

This complete laboratory reference manual explains the principles behind solid phase extraction (SPE) and provides readily reproducible protocols for solving extraction problems in forensic and clinical chemistry. Numerous actual chromatograms, based on original research and diverse applications, demonstrate the technique and the results that can be achieved. Extensive appendices allow fast access to frequently needed information on reagents, the preparation of solutions and buffers, milliequivalent and millimode calculations, buffers and pKa for SPE, and a complete RapidTrace® technical manual. Each proven protocol is described in step-by-step detail and contains an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and on avoiding known pitfalls.

Preface	p. v
Dedication	p. vii
Acknowledgments	p. xi
Chapter 1 Introduction to Solid Phase Extraction	p. 1
Chapter 2 Silica-Based Solid Phase Extraction	p. 41
Chapter 3 The Chemistry of Bonded Silicas	p. 55
Chapter 4 Bonded Phase Sorbent Synthesis	p. 63
Chapter 5 Strategies for the Use of Solid Phase Extraction	p. 91
Chapter 6 Optimizing Solid Phase Extraction Methods	p. 109
Chapter 7 Separation of Analytes from Their Matrices	p. 129
Chapter 8 Solid Phase Extraction Troubleshooting	p. 147
Chapter 9 Drug Methods for the Toxicology Lab	p. 169
Chapter 10 Selected Solid Phase Extraction Applications in Drug Analysis	p. 245
Chapter 11 General Methods for Drug Isolation	p. 281
Chapter 12 Chemical Derivatization	p. 297
Appendix A Glossary	p. 311
Appendix B Reagents	p. 315
Appendix C Preparation of Solutions and Buffers	p. 317
Appendix D Milliequivalent and Millimole Calculations	p. 321
Appendix E Selected Solvent Polarity and Property Scale	p. 323
Appendix F Buffers for Solid Phase Extraction	p. 325
Appendix G pK[subscript a] Reference for Solid Phase Extraction	p. 327
Appendix H RapidTrace Operating Guide	p. 335
Appendix I Further Reading	p. 343
Index	p. 351

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