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Library | Item Barcode | Call Number | Material Type | Item Category 1 | Status |
|---|---|---|---|---|---|
Searching... | 30000010102714 | TP248.27.Y43 Y426 2006 | Open Access Book | Book | Searching... |
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Summary
Summary
In this second edition of a widely used classic laboratory manual, leading experts utilize the tremendous progress and technological advances that have occurred to create a completely new collection of not only the major basic techniques, but also advanced protocols for yeast research and for using yeast as a host to study genes from other organisms. The authors provide detailed methods for the isolation of subcellular components-including organelles and macromolecules, for the basic cellular and molecular analysis specific for yeast cells, and for the creation of conditional mutant phenotypes that lend themselves to powerful genome manipulation. Additional protocols offer advanced approaches to study genetic interactions, DNA and chromatin metabolism, gene expression, as well as the foreign genes and gene products in yeast cells.
Table of Contents
| Basic Investigations in Saccharomyces cerevisiaeBrendan P. G. Curran and Virginia Bugeja |
| Isolation of Nucleic AcidsMichelle Hanna and Wei Xiao |
| Purification of Yeast PeroxisomesBen Distel and Astrid Kragt |
| Isolation of Yeast Plasma MembranesBarry Panaretou and Peter Piper |
| Isolation of Yeast MitochondriaChris Meisinger and Nikolaus Pfanner and Kaye N. Truscott |
| Extraction of Yeast LipidsRoger Schneiter and Gnther Daum |
| Two-Dimensional Gel Electrophoresis of Total Yeast ProteinsHlian Boucherie and Christelle Monribot-Espagne |
| Pulsed-Field Gel Electrophoresis of Budding Yeast ChromosomesLaura Maringele and David Lydall |
| Analysis of Yeast LipidsRoger Schneiter and Gnther Daum |
| Yeast Fluorescence MicroscopyJir Hasek |
| Intracellular Expression of Recombinant Antibody Fluorescent Protein Fusions for Localization of Target Antigens in Schizosaccharomyces pombeMichelle A. Alting-Mees and Eddy P. Risseeuw and Enwu Liu and Michel Desautels and William A. Crosby and Sean M. Hemmingsen |
| Yeast Transformation by the LiAc/SS Carrier DNA/PEG MethodR. Daniel Gietz and Robin A. Woods |
| MutagenesisLeslie Barbour and Michelle Hanna and Wei Xiao |
| Gene Disruption in the Budding Yeast Saccharomyces cerevisiaeJohannes H. Hegemann and Ulrich Gldener and Gabriele J. Khler |
| Inducible Degron and Its Application to Creating Conditional MutantsR. Jrgen Dohmen |
| Synthetic Lethal ScreenLeslie Barbour and Wei Xiao |
| Synthetic Genetic Array Analysis in Saccharomyces cerevisiaeAmy Hin Yan Tong and Charles Boone |
| Two-Dimensional Agarose Gel Analysis of DNA Replication IntermediatesAlain T. Dandjinou and Michel Larrive and Ralf E. Wellinger and Raymund J. Wellinger |
| Chromatin Assembly in a Crude Fraction From Yeast CellsKaren M. Robinson and Michael C. Schultz |
| Chromatin Immunoprecipitation to Study Protein-DNA Interactions in Budding YeastElena Ezhkova and William P. Tansey |
| Isolation of Yeast Nuclei and Micrococcal Nuclease Mapping of Nucleosome PositioningZhengjian Zhang and Joseph C. Reese |
| Study of Transcriptional Regulation Using a Reporter Gene AssayYu Fu and Wei Xiao |
| Assessing Telomeric PhenotypesCatherine LeBel and Michel Larrive and Amadou Bah and Nancy Laterreur and Nancy Lvesque and Raymund J. Wellinger |
| Controlled Expression of Recombinant Genes and Preparation of Cell-Free Extracts in YeastZhigang Wang |
| Production of Heterologous Proteins in Yeast With the Aid of the Hsp150D CarrierMarja Makarow and Anna-Liisa H'nnninen and Taina Suntio and Ricardo Nunes Bastos |
| Yeast Two-Hybrid System ScreeningR. Daniel Gietz |
| Import of Precursor Proteins Into Isolated Yeast MitochondriaNils Wiedemann and Nikolaus Pfanner and Peter Rehling |
| Index |
