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Library | Item Barcode | Call Number | Material Type | Item Category 1 | Status |
|---|---|---|---|---|---|
Searching... | 30000005201623 | QD79.C454 H63 1997 | Open Access Book | Book | Searching... |
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Summary
Summary
Providing a source of information on the powerful separation techniques high performance liquid chromatography and capillary electrophoresis, this text presents the fundamental theory, instrumentation, modes of operation, and optimization of these separations. Also provided is coverage of troubleshooting techniques, improvement of resolution, data manipulation, selectivity, and sensitivity. Information is also included on special procedures such as chiral separations, affinity, chromatography, and sample preparation and current trends such as miniaturization.
Reviews 1
Choice Review
This monograph outlines the basic concepts, the chemistry, and the instrumentation of high performance liquid chromatography (HPLC) and capillary electrophoresis (CE). In addition, these two methods are compared in order to assist the practitioner in solving actual chemical separation problems. The first three chapters provide a clear, though rather brief, overview of the theory and practice of HPLC. Included in this section are such topics as normal and reverse phase separations, as well as ion exchange, size exclusion and affinity chromatography, and chiral separations. The chapter on HPLC instrumentation is particularly useful because of the many clear figures and the valuable troubleshooting guide. Chapters 4 through 6 present the theory and instrumentation of a variety of CE techniques such as capillary zone electrophoresis (CZE), micellar electrokinetic capillary chromatography (MECC), capillary gel electrophoresis (CGE), capillary electrochromatography (CEC), capillary isoelectric focusing (CIEF), and chiral capillary electrophoresis. The remaining two chapters discuss the topics of data manipulation and miniaturization. Extensive chapter references; useful index. A well written "must" for all users of HPLC and/or CE and for nonpractitioners who wish a basic understanding of these important separation techniques. Upper-division undergraduates through professionals. H. T. McKone Saint Joseph College
Table of Contents
| High Performance Liquid Chromatography, Introduction |
| Classification of Liquid Chromatographic Methods |
| Basic Concepts |
| Summary of Major Concepts |
| References |
| Separations in High Performance Liquid Chromatography, Introduction |
| Normal Phase Chromatography |
| Reversed Phase Chromatography |
| Hydrophobic Interaction |
| Ion Exchange Chromatography |
| Size Exclusion Chromatography |
| Affinity Chromatography |
| Chiral Separations |
| Summary of Major Concepts |
| References |
| Instrumentation for High Performance Liquid Chromatography, Introduction |
| Solvent Delivery Systems |
| Solvent Degassing |
| Isocratic Versus Gradient Pumping Systems |
| Sample Introduction |
| Columns |
| Detectors |
| Sample Preparation |
| Troubleshooting |
| Summary of Major Concepts |
| References |
| Capillary Electrophoresis, Introduction |
| Classification of Electrophoretic Modes |
| Basic Concepts |
| Summary of Major Concepts |
| Separations in Capillary Electrophoresis, Introduction |
| Capillary Zone Electrophoresis |
| Micellar Electrokinetic Capillary Chromatography |
| Capillary Gel Electrophoresis |
| Capillary Electromatography |
| Chiral Capillary Electrophoresis |
| Capillary Isoelectric Focusing |
| Capillary Isotachophoresis |
| Summary of Major Concepts |
| References |
| Instrumentation for Capillary Electrophoresis, Introduction |
| High Voltage Power Supply |
| Sample Injection |
| Capillaries |
| Detection |
| Fraction Collection |
| Sample Preparation |
| Troubleshooting |
| Summary of Major Concepts |
| References |
| Data Manipulation, Introduction |
| Identification of Peaks |
| Quantification |
| Summary of Major Concepts |
| References |
| Miniaturization, Introduction |
| Classification |
| Applications of Miniaturized LC |
| Separations on a Chip |
| Summary of Major Concepts |
| References |
