Cover image for Priniciples and practice of bioanalysis
Title:
Priniciples and practice of bioanalysis
Edition:
2nd ed.
Publication Information:
London : CRC Press, 2008
Physical Description:
xiii, 326 p. : ill. ; 25 cm.
ISBN:
9780849338571
Added Author:

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30000010196703 RS189 P74 2008 Open Access Book Book
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30000010207651 RS189 P74 2008 Open Access Book Book
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Summary

Summary

In the seven years since the publication of Principles and Practice of Bioanalysis bioanalytical methods have remained the same, but their usage patterns have changed. This second edition of a bestseller provides an updated guide to the techniques used in developing and running ultra-trace analyses for drugs, metabolites, and other substances.

Extensively revised, this volume reflects the dynamic growth in methodologies that has occurred over the past five years. Each chapter has been overhauled or re-written to reflect modern practice. Experts in the field offer a logical path through the problems of small molecule bioanalysis, enabling you to choose the appropriate methods of analysis across a number of fields, including the agrochemical and pharmaceutical industries. They cover a wide range of analytical methods, including mass spectrometry, liquid chromatography, immunoassay, NMR, and gas chromatography.

Presenting the information in an informal and highly accessible style, the book covers the theory and practice of bioanalysis, enabling you to gain maximum benefit and to optimize analytical methods. With practical advice and guidance, it delineates how to develop robust analytical methods for a range of compounds, both natural and synthetic. Using these insights, you can more easily recognize problems and quickly solve them.


Table of Contents

Hugh Wiltshire and Angus NeddermanChristopher A. JamesAndy Gray and Peter LawrenceDavid BakesKenneth C. Saunders and Richard F. VennPeter Andrew and Mira V. DoigHugh Wiltshire and Angus NeddermanRichard F. VennKenneth C. Saunders and Christopher A. JamesMira V. Doig and David M. HigtonDavid M. Higton and Mira V. DoigGordon DearPhil GilbertGordon Dear and Angus Nedderman and Hugh WiltshireDavid Bakes
Preface to the Second Editionp. vii
Preface to the First Editionp. ix
About the Editorp. xi
Contributorsp. xiii
Chapter 1 Physicochemical Properties of Drugs and Metabolites and Their Impact on Bioanalysisp. 1
Chapter 2 Sample Preparationp. 19
Chapter 3 Basic HPLC Theory and Practicep. 41
Chapter 4 HPLC Optimisationp. 69
Chapter 5 HPLC Detectorsp. 99
Chapter 6 Gas Chromatography: What It Is and How We Use Itp. 123
Chapter 7 Thin-Layer Chromatographyp. 141
Chapter 8 Immunoassay Techniquesp. 151
Chapter 9 Automation of Sample Preparationp. 177
Chapter 10 Fundamental Aspects of Mass Spectrometryp. 193
Chapter 11 Quantitative LC-MSp. 223
Chapter 12 Mass Spectrometric Identification of Metabolitesp. 237
Chapter 13 Nuclear Magnetic Resonance in Drug Metabolismp. 261
Chapter 14 Metabolite Isolationp. 285
Chapter 15 Strategy for the Development of Quantitative Bioanalytical Proceduresp. 303
Indexp. 317