Protocols for neural cell culture

Since the conception of this acclaimed series of volumes examining neural tissue culture, the expansion of neuroscience has continued to produce vital discoveries that utilize tissue culture methodologies. The expert contributors to the fourth edition of Protocols for Neural Cell Culture refine existing protocols and present the emerging new techniques and culture media formulations linked with the many advances in neuroscience. While highlighting updated experimental procedures for many of the classical neural tissue culture preps, the volume addresses topics such as regenerative medicine and the methods to grow and expand embryonic and adult neural stem cells. Essential techniques for the isolation, expansion, and cryopreservation of neural tissue from mouse, rat, and human sources are covered, as well as methods to assess aspects of cell function (calcium imaging) and cell death. With its detailed, step-by-step laboratory protocols, this volume provides the kind of detailed description and implementation advice that is crucial for getting optimal results.

Authoritative and cutting-edge, Protocols for Neural Cell Culture, Fourth Edition continues the previous editions' tradition of exceptional quality and thorough coverage in tissue culture protocols for today's neuroscience in order to inspire researchers in the field to further explore this imperative area of study.

Sharon A. Louis and Brent A. ReynoldsMarcos R. Costa and Ravi Jagasia and Benedikt BerningerSoojung Shin and Mohan VemuriMirella Dottori and Alice Pébay and Martin F. PeraManon Blain and Veronique E. Miron and Caroline Lambert and Peter J. Darlington and Qiao-Ling Cui and Philippe Saikali and V. Wee Yong and Jack P. AntelBehnam A. Baghbaderani and Arindom Sen and Michael S. Kallos and Leo A. BehieNavjot Kaur and David V. Thompson and David Judd and David R. Piper and Richard G. Del MastroFrancine Nault and Paul De KoninckAndrea M. Vincent and Eva L. FeldmanFrancisco L.A.F. Gomes and Michel CayouetteJean de Vellis and Cristina A. Ghiani and Ina B. Wanner and Ruth ColeAkiko Nishiyama and Ryusuke Suzuki and Hao Zuo and Xiaoqin ZhuMakoto Sawada and Hiromi SuzukiSergey Fedoroff and Arleen RichardsonHaesun A. Kim and Patrice MaurelShelley Jacobs and Laurie C. DoeringJosef P. KapfhammerJesse E. Hanson and Adrienne L. Orr and Silvia Fernandez-Illescas and Ricardo A. Valenzuela and Daniel V. MadisonSaulius Satkauskas and Arnaud Muller and Morgane Roth and Dominique BagnardAlexandre BonninSean P. CreganSabine Bavamian and Eliana Scemes and Paolo MedaArleen Richardson and Sergey Fedoroff

Preface	p. v
Contributors	p. ix
1 Neurosphere and Neural Colony-Forming Cell Assays	p. 1
2 Directed Neuronal Differentiation of Embryonic and Adult-Derived Neurosphere Cells	p. 29
3 Culture and Differentiation of Human Neural Stem Cells	p. 51
4 Neural Differentiation of Human Embryonic Stem Cells	p. 75
5 Isolation and Culture of Primary Human CNS Neural Cells	p. 87
6 Bioengineering Protocols for Neural Precursor Cell Expansion	p. 105
7 Intracellular Calcium Assays in Dissociated Primary Cortical Neurons	p. 125
8 Dissociated Hippocampal Cultures	p. 137
9 Primary Sensory and Motor Neuron Cultures	p. 161
10 Retinal Cell and Tissue Culture	p. 175
11 Preparation of Normal and Reactive Astrocyte Cultures	p. 193
12 Oligodendrocyte Progenitor Cell Culture	p. 217
13 Isolation of Microglia Subpopulations	p. 231
14 Microglia from Progenitor Cells in Mouse Neopallium	p. 241
15 Primary Schwann Cell Cultures	p. 253
16 Primary Dissociated Astrocyte and Neuron Co-culture	p. 269
17 Cerebellar Slice Cultures	p. 285
18 Hippocampal Slice Cultures	p. 299
19 Molecular Substrates for Growing Neurons in Culture	p. 313
20 Guidance and Outgrowth Assays for Embryonic Thalamic Axons	p. 329
21 Detection of Cell Death in Neuronal Cultures	p. 343
22 Live Imaging of Neural Cell Functions	p. 353
23 Tissue Culture Procedures and Tips	p. 375
Index	p. 391

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