Principles and techniques of biochemistry and molecular biology

This is a new and expanded edition of the bestselling Principles and Techniques of Practical Biochemistry. It includes discussion of the theoretical principles, practical details and applications of the key experimental techniques that are routinely used in modern biochemistry and molecular biology, including those that have led to the emergence of the new disciplines of genomics, proteomics and bioinformatics. All chapters have been updated and new sections added to cover the principles of experimental design, the statistical analysis of quantitative analytical data, and the principles and practice of clinical biochemistry. here are new chapters covering cell culture, microscopy and mass spectrometry. To enhance student understanding of each topic, in-text worked examples are included in each chapter. The textbook will be essential reading for all bioscience students and pre-clinical medical students.

Preface

List of contributors

List of abbreviations

1 Basic priniciples Keith Wilson and John Fyffe

2 Cell culture techniques Anwar R. Baydoun

3 Centrifugation Kay Ohlendieck

4 Microscopy Stephen W. Paddock

5 Molecular biology, bioinformatics and basic techniques Ralph Rapley

6 Recombinant DNA and genetic analysis Ralph Rapley

7 Immunochemical techniques Robin Thorpe and Susan Thorpe

8 Protein structure, purification, characterisation and functional analysis John Walker

9 Mass spectrometric techniques Alistair Aitken

10 Electrophoretic techniques John Walker

11 Chromatographic techniques Keith Wilson

12 Spectroscopic techniques I: atomic and molecular electronic spectroscopy Derek B. Gordon

13 Spectroscopic techniques II: vibrational spectroscopy and electron and nuclear spin orientation in magnetic fields

14 Radioisotope techniques Robert J. Slater

15 Enzymes Keith Wilson

16 Cell membrane receptors and cell signalling Keith Wilson

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Summary

Summary

Table of Contents