Mesenchymal stem cells : methods and protocols

For over forty years, mesenchymal stem cells (MSCs) have been scrutinized and studied, garnering much attention due to their broad therapeutic efficacy. In Mesenchymal Stem Cells: Methods and Protocols, leaders in the field were assembled to contribute detailed methodologies for the isolation and characterization of human and rodent MSCs. Recently, these vital cells have shown therapeutic benefits in the treatment of myocardial infarction, stroke, lung diseases, spinal cord injury and other neurological disorders, thus promising a boundless future in their study.

Cutting edge and easy to use, Mesenchymal Stem Cells: Methods and Protocols is the perfect resource for scientists attempting to pursue this important and ever-developing field of research.

Margaret Wolfe and Radhika Pochampally and William Swaney and Roxanne L. RegerMark F. PittengerStan Gronthos and Andrew C. W. ZannettinoJohn K. Fraser and Min Zhu and Isabella Wulur and Zeni AlfonsoSeverine G. Dubois and Elizabeth Z. Floyd and Sanjin Zvonic and Gail Kilroy and Xiying Xu and Stacy Carling and Yuan Di C. Halvorsen and Eric Ravussin and Jeffrey M. GimbleRadhika PochampallyRoxanne L. Reger and Alan H. Tucker and Margaret R. WolfeRoxanne L. Reger and Margaret R. WolfeBeerelli SeshiJoni Ylostalo and Radhika Pochampally and Darwin J. ProckopReza Izadpanah and Bruce A. BunnellDonald G. Phinney

Preface	p. v
Contributors	p. xi
Color Plates	p. xv
Part I Isolation of Human Multipotential Stromal Cells from Bone Marrow and Adipose Tissue
Chapter 1 Isolation and Culture of Bone Marrow-Derived Human Multipotent Stromal Cells (hMSCs)	p. 3
Chapter 2 Mesenchymal Stem Cells from Adult Bone Marrow	p. 27
Chapter 3 A Method to Isolate and Purify Human Bone Marrow Stromal Stem Cells	p. 45
Chapter 4 Adipose-Derived Stem Cells	p. 59
Chapter 5 Isolation of Human Adipose-derived Stem Cells from Biopsies and Liposuction Specimens	p. 69
Part II Manipulation of Human Multipotential Stromal Cells
Chapter 6 Colony Forming Unit Assays for MSCs	p. 83
Chapter 7 Differentiation and Characterization of Human MSCs	p. 93
Chapter 8 Freezing Harvested hMSCs and Recovery of hMSCs from Frozen Vials for Subsequent Expansion, Analysis, and Experimentation	p. 109
Chapter 9 Gene Expression Analysis at the Single Cell Level Using the Human Bone Marrow Stromal Cell as a Model: Sample Preparation Methods	p. 117
Chapter 10 Assays of MSCs with Microarrays	p. 133
Chapter 11 Gene Delivery to Mesenchymal Stem Cells	p. 153
Part III Isolation and Characterization of Murine Multipotential Stromal Cells from Bone Marrow
Chapter 12 Isolation of Mesenchymal Stem Cells from Murine Bone Marrow by Immunodepletion	p. 171
Index	p. 187

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