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Library | Item Barcode | Call Number | Material Type | Item Category 1 | Status |
|---|---|---|---|---|---|
Searching... | 30000010150803 | QR82.R45 A37 2006 v.1 | Open Access Book | Great Book | Searching... |
Searching... | 30000010150804 | QR82.R45 A37 2006 v.2 | Open Access Book | Great Book | Searching... |
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Summary
Summary
Agrobacterium tumefaciens is a soil bacterium that for more than a century has been known as a pathogen causing the plant crown gall disease. Unlike many other pathogens, Agrobacterium has the ability to deliver DNA to plant cells and permanently alter the plant genome. The discovery of this unique feature 30 years ago has provided plant scientists with a powerful tool to genetically transform plants for both basic research purposes and for agricultural development. Compared to physical transformation methods such as particle bomba- ment or electroporation, Agrobacterium-mediated DNA delivery has a number of advantages. One of the features is its propensity to generate a single or a low copy number of integrated transgenes with defined ends. Integration of a single transgene copy into the plant genome is less likely to trigger "gene silencing" often associated with multiple gene insertions. When the first edition of Agrobacterium Protocols was published in 1995, only a handful of plants could be routinely transformed using Agrobacterium. Agrobacterium-mediated transformation is now commonly used to introduce DNA into many plant species, including monocotyledon crop species that were previously considered non-hosts for Agrobacterium. Most remarkable are recent developments indicating that Agrobacterium can also be used to deliver DNA to non-plant species including bacteria, fungi, and even mammalian cells.
Table of Contents
| Preface | p. vii |
| Contributors | p. xiii |
| Contents for Volume 1 | p. xxi |
| Part I Root Plants | |
| 1 Carrot (Daucus carota L.) | p. 3 |
| 2 Cassava (Manihot esculenta Crantz) | p. 13 |
| 3 Potato (Solanum tuberosum L.) | p. 25 |
| 4 Sweet Potato [Ipomoea batatas (L.) Lam.] | p. 37 |
| Part II Turf Grasses | |
| 5 Bermudagrass (Cynodon spp.) | p. 47 |
| 6 Perennial Ryegrass (Lolium perenne L.) | p. 55 |
| 7 Switchgrass (Panicum virgatum L.) | p. 65 |
| 8 Tall Fescue (Festuca arundinacea Schreb.) | p. 75 |
| 9 Turf Grasses | p. 83 |
| Part III Woody Species | |
| 10 American Elm (Ulmus americana) | p. 99 |
| 11 Cork Oak Trees (Quercus suber L.) | p. 113 |
| 12 Eucalyptus | p. 125 |
| 13 Pine (Pinus radiata) | p. 135 |
| 14 Poplar (Populus spp.) | p. 143 |
| 15 Rubber Tree (Hevea brasiliensis Muell. Arg) | p. 153 |
| Part IV Tropic Plants | |
| 16 Banana (Musa sp.) | p. 167 |
| 17 Citrus | p. 177 |
| 18 Coffee (Coffea sp.) | p. 191 |
| 19 Papaya (Carica papaya L.) | p. 209 |
| 20 Pineapple [Ananas comosus (L.) Merr.] | p. 219 |
| 21 Sugarcane (Saccharum spp.) | p. 227 |
| Part V Nuts and Fruits | |
| 22 American Chestnut [Castanea dentata (Marsh.) Borkh.] | p. 239 |
| 23 Apple (Malus x domestica) | p. 253 |
| 24 Blueberry (Vaccinium corymbosum L.) | p. 263 |
| 25 Grapevine (Vitis vinifera L.) | p. 273 |
| 26 Strawberry (Fragaria x ananassa) | p. 287 |
| 27 Walnut (Juglans) | p. 297 |
| Part VI Ornamental Plants | |
| 28 Carnation (Dianthus caryophylus L.) | p. 311 |
| 29 Chrysanthemum (Dendranthema x grandiflora) | p. 321 |
| 30 Orchids (Cymbidium spp., Oncidium, and Phalaenopsis) | p. 331 |
| 31 Petunia (Petunia hybrida) | p. 339 |
| 32 Rose (Rosa hybrida L.) | p. 351 |
| Part VII Medicinal Plants | |
| 33 Ginseng (Panax ginseng) | p. 361 |
| 34 Hemp (Cannabis sativa L.) | p. 373 |
| 35 Opium Poppy (Papaver somniferum) | p. 383 |
| Part VIII Non-Plants | |
| 36 Actinomycetes (Streptomyces lividans) | p. 395 |
| 37 Filamentous Fungi (Magnaporthe grisea and Fusarium oxysporum) | p. 403 |
| 38 Green Alga (Chlamydomonas reinhardtii) | p. 421 |
| 39 Mammalian Cells | p. 435 |
| 40 Mushroom (Agaricus bisporus) | p. 453 |
| 41 Yeast (Saccharomyces cerevisiae) | p. 465 |
| Index | p. 475 |
