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Summary
Summary
Biosensors combine biological recognition elements and signal conversion elements into a biodetection system. They have been developed for a wide variety of biodetection applications, offering the advantages of increased speed and ease of use compared to traditional detection methods. In Biosensors and Biodetection: Methods and Protocols, leading experts describe the major technologies in the field in extensive technical detail, allowing readers both to understand the technology and to construct similar devices. Volume 1: Optical-Based Detectors delves into direct and indirect optical detectors, including methods involving surface plasmon resonance, interferometric sensors, CCD based detectors, and spectrometers, among many other cutting-edge technologies. Written in the highly successful Methods in Molecular Biology(tm) series format, chapters include brief introductions to the subjects, lists of the necessary materials, step-by-step, readily reproducible protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls.
Comprehensive and up-to-date, Biosensors and Biodetection: Methods and Protocols is an ideal, user-friendly guide to this vital, versatile technology and a perfect tool for those who wish to further the field.
Table of Contents
Preface | p. v |
Contributors | p. xiii |
Contents of Volume 504 | p. xvii |
Part I Optical-based Detectors | |
1 Surface Plasmon Resonance and Surface Plasmon Field-Enhanced Fluorescence Spectroscopy for Sensitive Detection of Tumor Markers | p. 3 |
2 Surface Plasmon Resonance Biosensor for Biomolecular Interaction Analysis Based on Spatial Modulation Phase Detection | p. 21 |
3 Array-Based Spectral SPR Biosensor: Analysis of Mumps Virus Infection | p. 37 |
4 Optical Biosensors Based on Photonic Crystal Surface Waves | p. 49 |
5 Surface Plasmon Resonance Biosensing | p. 65 |
6 Label-Free Detection with the Resonant Mirror Biosensor | p. 89 |
7 Label-Free Detection with the Liquid Core Optical Ring Resonator Sensing Platform | p. 139 |
8 Reflectometric Interference Spectroscopy | p. 167 |
9 Phase Sensitive Interferometry for Biosensing Applications | p. 179 |
10 Label-Free Serodiagnosis on a Grating Coupler | p. 189 |
Part II Indirect Detectors | |
11 CCD Camera Detection of HIV Infection | p. 203 |
12 Simple Luminescence Detector for Capillary Electrophoresis | p. 221 |
13 Optical System Design for Biosensors Based on CCD Detection | p. 239 |
14 A Simple Portable Electroluminescence Illumination-Based CCD Detector | p. 259 |
15 Fluoroimmunoassays Using the NRL Array Biosensor | p. 273 |
16 Biosensors Technologies: Acousto-Optic Tunable Filter-Based Hyperspectral and Polarization Imagers for Fluorescence and Spectroscopic Imaging | p. 293 |
17 Photodiode-Based Detection System for Biosensors | p. 307 |
18 Photodiode Array On-chip Biosensor for the Detection of E. coli O157:H7 Pathogenic Bacteria | p. 325 |
19 DNA Analysis with a Photo-Diode Array Sensor | p. 337 |
20 Miniaturized and Integrated Fluorescence Detectors for Microfluidic Capillary Electrophoresis Devices | p. 361 |
21 Photomultiplier Tubes in Biosensors | p. 375 |
22 Integrating Waveguide Biosensor | p. 389 |
23 Detection of Fluorescence Generated in Microfluidic Channel Using In-Fiber Grooves and In-Fiber Microchannel Sensors | p. 403 |
24 Multiplex Integrating Waveguide Sensor: Signalyte-II | p. 423 |
25 CCD Based Fiber-Optic Spectrometer Detection | p. 435 |
Index | p. 447 |