Title:
Receptor signal transduction protocols
Series:
Methods in molecular biology ; 259
Publication Information:
Totowa, NJ : Humana Press, 2004
ISBN:
9781588293299
Available:*
Library | Item Barcode | Call Number | Material Type | Item Category 1 | Status |
---|---|---|---|---|---|
Searching... | 30000010164136 | QH603.C43 R42 2004 | Open Access Book | Book | Searching... |
On Order
Summary
Summary
presents a diverse array of methodologies employed to interrogate ligand- includes five
Table of Contents
Preface | p. v |
Preface to the First Edition | p. vi |
Contributors | p. xi |
1 Radioligand-Binding Methods for Membrane Preparations and Intact Cells | p. 1 |
2 Determination of Allosteric Interactions Using Radioligand-Binding Techniques | p. 29 |
3 Generation, Use, and Validation of Receptor-Selective Antibodies | p. 47 |
4 Immunocytochemical Identification of G-Protein-Coupled Receptor Expression and Localization | p. 67 |
5 Generation and Use of Epitope-Tagged Receptors | p. 81 |
6 Identification of G-Protein-Coupled Receptor mRNA Expression by Northern Blotting and In Situ Hybridization | p. 99 |
7 Electron Microscopic Studies of Receptor Localization | p. 123 |
8 Generation of Model Cell Lines Expressing Recombinant G-Protein-Coupled Receptors | p. 137 |
9 Viral Infection Protocols | p. 155 |
10 Expression of G-Protein Signaling Components in Adult Mammalian Neurons by Microinjection | p. 167 |
11 Covalent Modification of G-Proteins by Affinity Labeling | p. 183 |
12 Measurement of Agonist-Stimulated [superscript 35 S]GTP[gamma]S Binding to Assess Total G-Protein and G[alpha]-Subtype-Specific Activation by G-Protein-Coupled Receptors | p. 197 |
13 Identification and Quantitation of G-Protein [alpha]-Subunits | p. 207 |
14 Analysis of Function of Receptor--G-Protein and Receptor--RGS Fusion Proteins | p. 225 |
15 Assessment of Receptor Internalization and Recycling | p. 249 |
16 G-Protein-Coupled Receptor Phosphorylation and Palmitoylation | p. 275 |
17 Identification of G-Protein-Coupled Receptor Phosphorylation Sites by 2D Phosphopeptide Mapping | p. 283 |
18 Ubiquitination of G-Protein-Coupled Receptors | p. 299 |
19 Receptor Mutagenesis Strategies for Examination of Structure--Function Relationships | p. 307 |
20 Study of G-Protein-Coupled Receptor--Protein Interactions by Bioluminescence Resonance Energy Transfer | p. 323 |
21 Fluorescence Resonance Energy Transfer to Study Receptor Dimerization in Living Cells | p. 335 |
22 Identification of Protein Interactions by Yeast Two-Hybrid Screening and Coimmunoprecipitation | p. 353 |
23 Study of G-Protein-Coupled Receptor--Protein Interactions Using Gel Overlay Assays and Glutathione-S-Transferase--Fusion Protein Pull-Downs | p. 371 |
24 Receptor Knock-Out and Knock-In Strategies | p. 379 |
25 Statistical Methods in G-Protein-Coupled Receptor Research | p. 391 |
Index | p. 415 |