Cover image for Receptor signal transduction protocols
Title:
Receptor signal transduction protocols
Series:
Methods in molecular biology ; 259
Publication Information:
Totowa, NJ : Humana Press, 2004
ISBN:
9781588293299

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30000010164136 QH603.C43 R42 2004 Open Access Book Book
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Summary

Summary

presents a diverse array of methodologies employed to interrogate ligand- includes five


Table of Contents

David B. Bylund and Jean D. Deupree and Myron L. ToewsSebastian LazarenoJohn J. MackrillLaura C. Mongan and Blair D. GrubbR. A. Jeffrey McIlhinneyLucy F. DonaldsonRafael LujanEmmanuel HermansAntonio Porcellini and Antonio De BlasiStephen R. IkedaMartin Hohenegger and Michael Freissmuth and Christian NanoffMark R. Dowling and Stefan R. Nahorski and R. A. John ChallissIan Mullaney and Graeme MilliganRichard J. Ward and Graeme MilliganJennifer A. KoenigAndrew B. Tobin and Mark WheatleyAndree BlaukatAdriano Marchese and Jeffrey L. BenovicMarion Blomenrohr and Henry F. Vischer and Jan BogerdKaren M. Kroeger and Karin A. EidneJurgen E. Bader and Annette G. Beck-SickingerMichael Tanowitz and Mark von ZastrowAshley E. Brady and Qin Wang and Lee E. LimbirdIkuo Matsuda and Atsu AibaPat Freeman and Domenico Spina
Prefacep. v
Preface to the First Editionp. vi
Contributorsp. xi
1 Radioligand-Binding Methods for Membrane Preparations and Intact Cellsp. 1
2 Determination of Allosteric Interactions Using Radioligand-Binding Techniquesp. 29
3 Generation, Use, and Validation of Receptor-Selective Antibodiesp. 47
4 Immunocytochemical Identification of G-Protein-Coupled Receptor Expression and Localizationp. 67
5 Generation and Use of Epitope-Tagged Receptorsp. 81
6 Identification of G-Protein-Coupled Receptor mRNA Expression by Northern Blotting and In Situ Hybridizationp. 99
7 Electron Microscopic Studies of Receptor Localizationp. 123
8 Generation of Model Cell Lines Expressing Recombinant G-Protein-Coupled Receptorsp. 137
9 Viral Infection Protocolsp. 155
10 Expression of G-Protein Signaling Components in Adult Mammalian Neurons by Microinjectionp. 167
11 Covalent Modification of G-Proteins by Affinity Labelingp. 183
12 Measurement of Agonist-Stimulated [superscript 35 S]GTP[gamma]S Binding to Assess Total G-Protein and G[alpha]-Subtype-Specific Activation by G-Protein-Coupled Receptorsp. 197
13 Identification and Quantitation of G-Protein [alpha]-Subunitsp. 207
14 Analysis of Function of Receptor--G-Protein and Receptor--RGS Fusion Proteinsp. 225
15 Assessment of Receptor Internalization and Recyclingp. 249
16 G-Protein-Coupled Receptor Phosphorylation and Palmitoylationp. 275
17 Identification of G-Protein-Coupled Receptor Phosphorylation Sites by 2D Phosphopeptide Mappingp. 283
18 Ubiquitination of G-Protein-Coupled Receptorsp. 299
19 Receptor Mutagenesis Strategies for Examination of Structure--Function Relationshipsp. 307
20 Study of G-Protein-Coupled Receptor--Protein Interactions by Bioluminescence Resonance Energy Transferp. 323
21 Fluorescence Resonance Energy Transfer to Study Receptor Dimerization in Living Cellsp. 335
22 Identification of Protein Interactions by Yeast Two-Hybrid Screening and Coimmunoprecipitationp. 353
23 Study of G-Protein-Coupled Receptor--Protein Interactions Using Gel Overlay Assays and Glutathione-S-Transferase--Fusion Protein Pull-Downsp. 371
24 Receptor Knock-Out and Knock-In Strategiesp. 379
25 Statistical Methods in G-Protein-Coupled Receptor Researchp. 391
Indexp. 415