Elaine A. Elion and Yunmei WangMaofu Fu and Chenguang Wang and Xueping Zhang and Richard G. PestellYukihito Kabuyama and Kirsi K. Polvinen and Katheryn A. Resing and Natalie G. AhnDaniel J. Noonan and Kenneth Henry and Michelle L. TwaroskiPengbo ZhouJan Brabek and Steven K. HanksFrancesca Zappacosta and Michael J. Huddleston and Roland S. AnnanPaul C. Brandt and Thomas C. VanamanHogune Im and Jeffrey A. Grass and Kirby D. Johnson and Meghan E. Boyer and Jing Wu and Emery H. BresnickLaurent KurasDouglas A. AndresNatasha Elizabeth Zachara and Win Den Cheung and Gerald Warren HartJaeho Lee and Donghang Cheng and Mark T. BedfordGil-Soo Han and George M. CarmanGregory S. Taylor and Jack E. DixonChristina C. Leslie and Michael H. GelbHiroko Hama and Javad Torabinejad and Glenn D. Prestwich and Daryll B. DeWaldSandrine Evellin and Marco Mongillo and Anna Terrin and Valentina Lissandron and Manuela ZaccoloChitra Subramanian and Yao Xu and Carl Hirschie Johnson and Albrecht G. von ArnimFrank van Drogen and Matthias PeterNigel J. Waterhouse and Rohan Steel and Ruth Kluck and Joseph A. Trapani

Preface	p. v
Contributors	p. ix
1 Making Protein Immunoprecipitates	p. 1
2 Signal Transduction Inhibitors in Cellular Function	p. 15
3 Two-Dimensional Gel Electrophoresis for the Identification of Signaling Targets	p. 37
4 A High-Throughput Mammalian Cell-Based Transient Transfection Assay	p. 51
5 Determining Protein Half-Lives	p. 67
6 Assaying Protein Kinase Activity	p. 79
7 Comparative Phosphorylation Site Mapping From Gel-Derived Proteins Using a Multidimensional ES/MS-Based Approach	p. 91
8 Studies of Calmodulin-Dependent Regulation	p. 111
9 Measurement of Protein-DNA Interactions In Vivo by Chromatin Immunoprecipitation	p. 129
10 Characterization of Protein-DNA Association In Vivo by Chromatin Immunoprecipitation	p. 147
11 Nonradioactive Methods for Detecting Activation of Ras-Related Small G Proteins	p. 163
12 Nucleocytoplasmic Glycosylation, O-GlcNAc: Identification and Site Mapping	p. 175
13 Techniques in Protein Methylation	p. 195
14 Assaying Lipid Phosphate Phosphatase Activities	p. 209
15 Assaying Phosphoinositide Phosphatases	p. 217
16 Assaying Phospholipase A[subscript 2] Activity	p. 229
17 Measurement and Immunofluorescence of Cellular Phosphoinositides	p. 243
18 Measuring Dynamic Changes in cAMP Using Fluorescence Resonance Energy Transfer	p. 259
19 In Vivo Detection of Protein-Protein Interaction in Plant Cells Using BRET	p. 271
20 Revealing Protein Dynamics by Photobleaching Techniques	p. 287
21 Assaying Cytochrome c Translocation During Apoptosis	p. 307
Index	p. 315