Peptide characterization and application protocols

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Title:

Series:

Methods in molecular biology ; 386

Publication Information:

Totowa, NJ : Humana Press, 2007

ISBN:

9781588295507

Subject Term:

Peptides -- Analysis

Peptides -- Laboratory Manuals

Added Author:

Fields, Gregg B.

Available:*

Library	Item Barcode	Call Number	Material Type	Item Category 1	Status
Searching... PSZ JB	30000010161130	QP552.P4 P465 2007	Open Access Book	Book	Searching... Unknown

Peptide Characterization and Application Protocols is dedicated entirely to the characterization of peptides and their applications for the study of biochemical systems and the contributing authors are all leaders in the field of peptide research. Part I, Characterization, presents the most recent advances in select analytical techniques, including high-performance liquid chromatography (HPLC) for purification and evaluation of peptides and mass spectrometry (MS) for the analysis of standard and modified peptides. Innovative methods of synthesis and characterization of membrane peptides are also presented. Part II, Application, presents a variety of specific applications for synthetic peptides. The collected protocols demonstrate the systematic manner in which peptides can be constructed, analyzed, and applied to attack contemporary problems in biochemistry, and this book will be an indispensable aid in the pursuit of new directions in peptide research.

Colin T. Mant and Yuxin Chen and Zhe Yan and Traian V. Popa and James M. Kovacs and Janine B. Mills and Brian P. Tripet and Robert S. HodgesKathryn L. Stone and Myron Crawford and Walter McMurray and Nancy Williams and Kenneth R. WilliamsGary M. HathawayFred NaiderJanelle L. Lauer-Fields and Dmitriy Minond and Keith Brew and Gregg B. FieldsDiane Baronas-Lowell and Janelle L. Lauer-Fields and Mohammad Al-Ghoul and Gregg B. FieldsMarcus A. Etienne and Nadia J. Edwin and Jed P. Aucoin and Paul S. Russo and Robin L. McCarley and Robert P. HammerJason S. Lewis and Carolyn J. AndersonJosep Farrera-Sinfreu and Ernest Giralt and Miriam Royo and Fernando AlbericioEvonne M. Rezler and David R. Khan and Raymond Tu and Matthew Tirrell and Gregg B. FieldsSebastien Deshayes and Federica Simeoni and May C. Morris and Gilles Divita and Frederic HeitzLaszlo Otvos and Mare CudicMaciej Stawikowski and Predrag Cudic

Preface	p. v
Contributors	p. ix
Part I Characterization
1 HPLC Analysis and Purification of Peptides	p. 3
2 Identification of Proteins Based on MS/MS Spectra and Location of Posttranslational Modifications	p. 57
3 Determination of Phosphorylated and O-Glycosylated Sites by Chemical Targeting (CTID) at Ambient Temperature	p. 79
4 Synthesis, Biosynthesis, and Characterization of Transmembrane Domains of a G Protein-Coupled Receptor	p. 95
Part II Applications
5 Application of Topologically Constrained Mini-Proteins as Ligands, Substrates, and Inhibitors	p. 125
6 Proteolytic Profiling of the Extracellular Matrix Degradome	p. 167
7 [Beta]-Amyloid Protein Aggregation	p. 203
8 Radiometal-Labeled Somatostatin Analogs for Applications in Cancer Imaging and Therapy	p. 227
9 Cell-Penetrating Proline-Rich Peptidomimetics	p. 241
10 Peptide-Mediated Targeting of Liposomes to Tumor Cells	p. 269
11 Peptide-Mediated Delivery of Nucleic Acids into Mammalian Cells	p. 299
12 Broth Microdilution Antibacterial Assay of Peptides	p. 309
13 Depsipeptide Synthesis	p. 321
Index	p. 341

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Summary

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Table of Contents