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Library | Item Barcode | Call Number | Material Type | Item Category 1 | Status |
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Searching... | 30000010301134 | QP171 M385 2013 | Open Access Book | Book | Searching... |
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Summary
Summary
Metabolomics, the global characterisation of the small molecule complement involved in metabolism, has evolved into a powerful suite of approaches for understanding the global physiological and pathological processes occurring in biological organisms. The diversity of metabolites, the wide range of metabolic pathways and their divergent biological contexts require a range of methodological strategies and techniques. Methodologies for Metabolomics provides a comprehensive description of the newest methodological approaches in metabolomic research. The most important technologies used to identify and quantify metabolites, including nuclear magnetic resonance and mass spectrometry, are highlighted. The integration of these techniques with classical biological methods is also addressed. Furthermore, the book presents statistical and chemometric methods for evaluation of the resultant data. The broad spectrum of topics includes a vast variety of organisms, samples and diseases, ranging from in vivo metabolomics in humans and animals to in vitro analysis of tissue samples, cultured cells and biofluids.
Table of Contents
1 Exploring the human metabolome by NMR spectroscopy and mass spectroscopyDavid S. Wishart |
2 Methodological requirements for lipidomics researchKui Yang and Michael A. Kiebish and Richard W. Gross |
3 Biological methods for metabolic researchArancha Cebrian and Laura Menchen and Elsa Sanchez-Lopez and Juan Casado-Vela and Santiago Diaz-Moralli and Marta Casceante and Teresa Gomez del Pulgar and Juan Carlos Lacal |
4 Considerations in sample preparation, collection, and extraction approaches applied in microbial, plant, and mammalian metabolic profilingJ. William Allwood and Catherine L. Winder and Warwick B. Dunn and Royston Goodacre |
5 MS-based methodologies for single-cell metabolite detection and identificationAnn M. Knolhoff and Peter Nemes and Stanislav S. Rubakhin |
6 Direct metabolomics from tissues and cells: laser ablation electrospray ionization for small molecule and lipid characterizationAkos Vertes and Bindesh Shrestha and Peter Nemes |
7 Bioinformatic approaches to process and annotate high-resolution mass spectrometry dataRalf Weber and Mark Viant |
8 Approaches for natural product detection and structural elucidation using mass spectrometry with high mass accuracyIoanna Ntai and Neil L. Kelleher |
9 Metabolomics using ion mobility mass spectrometryKimberly A. Kaplan and Herbert Hill |
10 Metabolomics via biomedical mass spectrometry: from sampling to clinical applicationsBong Chul Chung and Man Ho Choi |
11 Analytical techniques in metabolomics integrating NMR and chromatography withMS U. Braumann and M. Godejohann |
12 NMR of CSF: the neurometabolomeFanny Mochel |
13 NMR analysis and genetic metabolic disease UdoF. H. Engelke and Angelina Goudswaard and Eva Morava and Ron Wevers |
14 NMR methods for metabolomic investigation of amniotic fluidAna M. Gil and Goncalo Graca |
15 Chemometric methods in NMR-based body fluid analysisRon Wehrens and Udo Engelke |
16 Lipid profiling in health and disease ChristinaE. Kostara and Eleni T. Bairaktari |
17 NMR-based saliva metabolomics HanneChristine Bertram and Morten Rahr Clausen |
18 Reproducible sample preparation and spectrum acquisition techniques for metabolic profiling of human tissues byH HR-MAS NMR Marital Pitto and F. M. Moussallieh and A. Imperiale and M. A. Benahmed and J. Detour and J. P. Bellocq and I. J. Namer and K. Elbayed |
19 MR spectroscopy in investigating the cancer metabolome in preclinical model systemsMarie-France Penet and Zaver M. Bhujwalla and Kristine Glunde |
20 Metabolomic magnetic resonance spectroscopy of human tissue: a comparison of in vivo and HR-MAS ex vivo techniquesGeoffery S. Payne and Yuen-Li Chung and Martin O. Leach |
21 Phospholipidomics by P NMR spectroscopy of tissue extractsNorbert W. Lutz and Patrick J. Cozzone |
22 C NMR for analysis of metabolic pathwaysCraig R. Malloy and Elizabeth Maher and Issac Marin-Valencia and Bruce Mickey and Ralph J. DeBerardinis and A. Dean Sherry |
23 Hyperpolarized NMR spectroscopy - a new method for metabolomic researchRalph E. Hurd and Yi-Fen Yen and Albert Chen |
24 Assignment strategies for NMR resonances in metabolomics researchTeresa W. M. Fan and rew N. Lane |