Fundamentals of enzymology : the cell and molecular biology of catalytic proteins

Title:

Personal Author:

Price, Nicholas C.

Edition:

3rd ed.

Publication Information:

Oxford : Oxford University Press, 1999

ISBN:

9780198502296

Subject Term:

Enzymes

Added Author:

Stevens, Lewis

Available:*

Library	Item Barcode	Call Number	Material Type	Item Category 1	Status
Searching... PSZ JB	30000010151238	QP601 P74 1999	Open Access Book	Book	Searching... Unknown

Since the publication of the successful and popular second edition of Fundamentals of Enzymology in 1989 there has been a large increase in the knowledge of several aspects of enzymology, not least the rapid acceleration of structural characterization of enzymes and the development of the field of bioinformatics. This new edition places appropriate emphasis on the new knowledge and consolidates the strengths of the previous editions. As before, Fundamentals of Enzymology 3rd ed gives an all-round view of the field including enzyme purification and characterization, enzyme structure (including information on the web), enzyme kinetics, the mechanisms and control of enzyme action, enzyme folding, how enzymes act in vivo, enzyme synthesis and degradation, and also clinical and industrial applications of enzymology. Throughout the book, the integration of these themes is stressed.

Author Notes

Nicholas Price is at University of Stirling. Lewis Stevens is at University of Stirling.

A note on units	p. xv
List of abbreviations	p. xvii
Chapter 1 Introduction	p. 1
1.1 Aims of this book	p. 1
1.2 Historical aspects	p. 1
1.3 Remarkable properties of enzymes as catalysts	p. 4
1.4 Cofactors	p. 7
1.5 Nomenclature and classification of enzymes	p. 8
1.6 The contents of this book	p. 13
References	p. 14
Chapter 2 The purification of enzymes	p. 15
2.1 Introduction	p. 15
2.2 Why isolate enzymes?	p. 15
2.3 Objectives and strategy in enzyme purification	p. 16
2.4 Choice of source	p. 17
2.5 Methods of homogenization	p. 20
2.6 Methods of separation	p. 21
2.7 How to judge the success of a purification procedure	p. 34
2.8 Examples of purification procedures	p. 36
2.9 Conclusions from the examples of enzyme purification	p. 43
References	p. 44
Chapter 3 The structure of enzymes	p. 47
3.1 Introduction	p. 47
3.2 The determination of M[subscript r]	p. 48
3.3 The determination of amino-acid composition and primary structure	p. 54
3.4 The determination of secondary and tertiary structure	p. 75
3.5 The determination of quaternary structure	p. 100
3.6 The unfolding and folding of enzymes	p. 106
3.7 Concluding remarks	p. 110
References	p. 111
Appendix 3.1	p. 116
References for Appendix 3.1	p. 117
Chapter 4 An introduction to enzyme kinetics	p. 118
4.1 Outline of the chapter	p. 118
4.2 How do we obtain kinetic data?	p. 119
4.3 How do we analyse kinetic data?	p. 121
4.4 Pre-steady-state kinetics	p. 144
4.5 Concluding remarks	p. 148
References	p. 148
Appendix 4.1	p. 150
Appendix 4.2	p. 151
Appendix 4.3	p. 152
References for Appendices	p. 153
Chapter 5 The mechanism of enzyme action	p. 154
5.1 Introduction	p. 154
5.2 Definition of the mechanism of an enzyme-catalysed reaction	p. 154
5.3 Background to catalysis	p. 155
5.4 Experimental approaches to the determination of enzyme mechanisms	p. 162
5.5 Examples of enzyme mechanisms	p. 177
5.6 Concluding comments on enzyme mechanisms	p. 212
References	p. 212
Chapter 6 The control of enzyme activity	p. 217
6.1 Introduction	p. 217
6.2 Control of the activities of single enzymes	p. 218
6.3 Control of metabolic pathways	p. 237
6.4 Examples of control of metabolic pathways	p. 249
6.5 Concluding remarks	p. 266
References	p. 266
Appendix 6.1	p. 270
Chapter 7 Enzymes in organized systems	p. 273
7.1 Introduction	p. 273
7.2 Organized enzyme systems	p. 273
7.3 RNA polymerase from E. coli	p. 274
7.4 The occurrence and isolation of multienzyme proteins	p. 281
7.5 Phylogenetic distribution of multienzyme proteins	p. 282
7.6 Properties of multienzyme proteins	p. 283
7.7 Pyruvate dehydrogenase multienzyme complex and related systems	p. 285
7.8 Glycine decarboxylase multienzyme complex	p. 294
7.9 The tryptophan synthase multienzyme complex from E. coli	p. 294
7.10 Carbamoyl phosphate synthase and the associated enzymes of the pyrimidine and arginine biosynthetic pathways in E. coli, fungi, and mammalian cells	p. 300
7.11 Multienzyme polypeptides: fatty-acid synthase and the arom complex (AROM enzyme)	p. 304
7.12 Enzymes involved in DNA synthesis	p. 315
7.13 The glycogen particle	p. 315
7.14 Conclusions	p. 317
References	p. 317
Chapter 8 Enzymes in the cell	p. 321
8.1 Introduction	p. 321
8.2 Intracellular compartmentation	p. 322
8.3 Compartmentation of metabolic pathways	p. 333
8.4 Vectorial organization of enzymes associated with membranes	p. 347
8.5 The concentrations of enzymes and substrates in vivo	p. 355
8.6 Conclusions	p. 366
References	p. 367
Chapter 9 Enzyme turnover	p. 370
9.1 Introduction	p. 370
9.2 Kinetics of enzyme turnover	p. 371
9.3 Methods for measurement of rates of enzyme turnover	p. 373
9.4 Results from measurements of rates of enzyme turnover	p. 375
9.5 Possible correlations between the rates of turnover and the structure and function of enzymes	p. 376
9.6 The mechanisms of protein degradation	p. 377
9.7 The significance of enzyme turnover	p. 389
9.8 Other processes in which intracellular proteolysis is important	p. 394
9.9 Conclusions	p. 396
References	p. 396
Appendix 9.1	p. 399
Chapter 10 Clinical aspects of enzymology	p. 400
10.1 Introduction	p. 400
10.2 Determination of enzyme activities for clinical diagnosis	p. 401
10.3 Clinical enzymology of liver disease	p. 404
10.4 Clinical enzymology of heart disease	p. 406
10.5 Other enzyme activities that become elevated in serum in disease	p. 408
10.6 The detection and significance of enzyme deficiencies	p. 411
10.7 Enzyme inhibitors and drug design	p. 415
10.8 The use of enzymes to determine the concentrations of metabolites of clinical importance	p. 416
10.9 Enzyme therapy	p. 424
10.10 Conclusions	p. 428
References	p. 428
Chapter 11 Enzyme technology	p. 430
11.1 Introduction	p. 430
11.2 Use of microorganisms in brewing and cheesemaking	p. 430
11.3 Use of microorganisms in the production of organic chemicals	p. 433
11.4 Use of isolated enzymes in industrial processes	p. 433
11.5 Immobilized enzymes	p. 440
References	p. 449
Appendix Enzymes referred to in Chapters 1-11	p. 450
Index	p. 471

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