Cover image for Flow cytometry principles for clinical laboratory practice : quality assurance for quantitative immunophenotyping
Title:
Flow cytometry principles for clinical laboratory practice : quality assurance for quantitative immunophenotyping
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Publication Information:
New York, NY : Wiley-Liss, 1995
ISBN:
9780471021766
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30000010129485 QR187.I486 O93 1995 Open Access Book Book
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Summary

Summary

FLOW CYTOMETRY PRINCIPLES FOR CLINICAL LABORATORY PRACTICE

Quality Assurance for Quantitative Immunophenotyping

Marilyn A. Owens and Michael R. Loken

Flow Cytometry Principles for Clinical Laboratory Practice is aninvaluable benchtop reference and introduction for first-time usersof flow cytometry in clinical diagnostic laboratories. This manualprovides authoritative coverage of accepted methods, qualitycontrols, and standards for the cytometric analysis of clinicalsamples with particular relevance to CD4+ lymphocytes. CD4+lymphocyte determination by immunophenotyping is the standardmethod for evaluating the status of HIV infection, and the singlemost frequent application of flow cytometry in a diagnosticsetting.

Flow Cytometry Principles for Clinical Laboratory Practice willappeal to clinical laboratory technologists, medical practitioners,and cytometry lab directors in their role as instructors of newpersonnel. In addition, it is a valuable resource for clinical andbiomedical researchers, establishing quantitative cytometricsystems for studies in immunology and hematology.


Author Notes

Marilyn A. Owens and Michael R. Loken are the authors of Flow Cytometry Principles for Clinical Laboratory Practice: Quality Assurance for Quantitative Immunophenotyping, published by Wiley.


Table of Contents

Assumptions in Flow Cytometry
Components of Flow Cytometric Immunophenotyping
Quality Control of Flow Cytometer and Reagents
Quantitative Immunophenotyping in HIV Infection
Peripheral Blood Stem Cell Quantitation
Variability in Immunophenotyping
Safety and Specimen Acquisition, Handling, and Processing
Analysis of FCI Results and Troubleshooting
New Methods for Cell Enumeration
Index