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Title:
PCR
Personal Author:
Edition:
2nd ed.
Publication Information:
New York, NY : Taylor & Francis, 2006
ISBN:
9780415355476
Added Author:

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Library
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Call Number
Material Type
Item Category 1
Status
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30000010129897 QP606.D46 M63 2006 Open Access Book Book
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Summary

Summary

A thoroughly updated version of the successful first edition with a new chapter on Real-Time PCR, more prokaryotic applications, and more detail in the complex mutagenesis sections. Information on PCR applications in genomics and proteomics have been expanded and integrated throughout the text. There is also advice on available products and specific pointers to the most appropriate methods. As with the first edition, this will be an ideal practical introduction and invaluable guide to PCR and its applications.


Author Notes

Dr. Simon Moller, Department of Biology, University of Leicester, UK

Professor Michael McPherson, School of Biochemistry and Microbiology, University of Leeds, UK


Table of Contents

Ch 1 An Introduction to PCR
Ch 2 Understanding PCR
Ch 3 Reagents and Instrumentation
Ch 4 Optimization of PCR
Ch 5 Analysis, Sequencing and in Vitro Expression Of PCR Products
Ch 6 Purification and Cloning Of PCR Products
Ch 7 PCR Mutagenesis
Ch 8 Analysis of Gene Expression
Ch 9 Real-Time PCR
Ch 10 Cloning Genes by PCR
Ch 11 Genome Analysis
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